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Epstein-Barr virus replicative gene transcription during de novo infection of human thymocytes: simultaneous early expression of BZLF-1 and its repressor RAZ.
Virology. 1995 Apr 20; 208(2):685-95.V

Abstract

Epstein-Barr virus (EBV) is known to infect B cells and epithelial cells. We and others have shown that EBV can also infect a subset of thymocytes. Infection of thymocytes was accompanied by the appearance of linear EBV genome within 8 hr of infection. Circularization of the EBV genome was not detected. This is in contrast to the infection in B cells where the genome can circularize within 24 hr of infection. The appearance of the BamHI ZLF-1 gene product, ZEBRA, by RT-PCR, was observed within 8 hr of infection. The appearance of a novel fusion transcript (RAZ), which comprised regions of the BZLF-1 locus and the adjacent BRLF-1 locus, was detected by RT-PCR. ZEBRA protein was also identified in infected thymocytes by immunoprecipitation. In addition, we demonstrated that the EBNA-1 gene in infected thymocytes was transcribed from the Fp promoter, rather than from the Cp/Wp promoter which is used in latently infected B cells. Transcripts encoding gp350/220, the major coat protein of EBV, were identified, but we did not find any evidence of transcription from the LMP-2A or EBER-1 loci in infected thymocytes. These observations suggest that de novo EBV infection of thymocytes differs from infection of B cells. The main difference is that with thymocytes, no evidence could be found that the virus ever circularizes. Rather, EBV remains in a linear configuration from which replicative genes are transcribed.

Authors+Show Affiliations

Department of Pediatrics, National Jewish Center for Immunology, Denver, Colorado 80206, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7747440

Citation

Kelleher, C A., et al. "Epstein-Barr Virus Replicative Gene Transcription During De Novo Infection of Human Thymocytes: Simultaneous Early Expression of BZLF-1 and Its Repressor RAZ." Virology, vol. 208, no. 2, 1995, pp. 685-95.
Kelleher CA, Paterson RK, Dreyfus DH, et al. Epstein-Barr virus replicative gene transcription during de novo infection of human thymocytes: simultaneous early expression of BZLF-1 and its repressor RAZ. Virology. 1995;208(2):685-95.
Kelleher, C. A., Paterson, R. K., Dreyfus, D. H., Streib, J. E., Xu, J. W., Takase, K., Jones, J. F., & Gelfand, E. W. (1995). Epstein-Barr virus replicative gene transcription during de novo infection of human thymocytes: simultaneous early expression of BZLF-1 and its repressor RAZ. Virology, 208(2), 685-95.
Kelleher CA, et al. Epstein-Barr Virus Replicative Gene Transcription During De Novo Infection of Human Thymocytes: Simultaneous Early Expression of BZLF-1 and Its Repressor RAZ. Virology. 1995 Apr 20;208(2):685-95. PubMed PMID: 7747440.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Epstein-Barr virus replicative gene transcription during de novo infection of human thymocytes: simultaneous early expression of BZLF-1 and its repressor RAZ. AU - Kelleher,C A, AU - Paterson,R K, AU - Dreyfus,D H, AU - Streib,J E, AU - Xu,J W, AU - Takase,K, AU - Jones,J F, AU - Gelfand,E W, PY - 1995/4/20/pubmed PY - 1995/4/20/medline PY - 1995/4/20/entrez SP - 685 EP - 95 JF - Virology JO - Virology VL - 208 IS - 2 N2 - Epstein-Barr virus (EBV) is known to infect B cells and epithelial cells. We and others have shown that EBV can also infect a subset of thymocytes. Infection of thymocytes was accompanied by the appearance of linear EBV genome within 8 hr of infection. Circularization of the EBV genome was not detected. This is in contrast to the infection in B cells where the genome can circularize within 24 hr of infection. The appearance of the BamHI ZLF-1 gene product, ZEBRA, by RT-PCR, was observed within 8 hr of infection. The appearance of a novel fusion transcript (RAZ), which comprised regions of the BZLF-1 locus and the adjacent BRLF-1 locus, was detected by RT-PCR. ZEBRA protein was also identified in infected thymocytes by immunoprecipitation. In addition, we demonstrated that the EBNA-1 gene in infected thymocytes was transcribed from the Fp promoter, rather than from the Cp/Wp promoter which is used in latently infected B cells. Transcripts encoding gp350/220, the major coat protein of EBV, were identified, but we did not find any evidence of transcription from the LMP-2A or EBER-1 loci in infected thymocytes. These observations suggest that de novo EBV infection of thymocytes differs from infection of B cells. The main difference is that with thymocytes, no evidence could be found that the virus ever circularizes. Rather, EBV remains in a linear configuration from which replicative genes are transcribed. SN - 0042-6822 UR - https://www.unboundmedicine.com/medline/citation/7747440/Epstein_Barr_virus_replicative_gene_transcription_during_de_novo_infection_of_human_thymocytes:_simultaneous_early_expression_of_BZLF_1_and_its_repressor_RAZ_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0042-6822(85)71200-7 DB - PRIME DP - Unbound Medicine ER -