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Cellular proteins specifically bind to the 5'-noncoding region of hepatitis C virus RNA.
Virology. 1995 Apr 20; 208(2):723-32.V

Abstract

Hepatitis C virus (HCV) RNA contains a highly conserved 5'-noncoding region (5'NCR) which may be important in viral multiplication. To study the possible mechanisms of the cellular proteins involved in HCV replication and pathogenesis, a gel mobility shift assay and competition analysis were performed with the HCV 5'NCR. Two specific complexes were formed between the 341-nucleotide RNA of the HCV 5'NCR and proteins of mammalian cells. The specific RNA-protein complexes were maintained in the region of the 5'NCR from nucleotides 131 to 253. Nevertheless, the slower migrating RNA-protein complex failed to form when a polypyrimidine tract sequence (191-UCCUUUCUU-199) in the stem-loop III structure of HCV 5'NCR was changed to 191-UCCUUUggU-199. A uv cross-linking assay further identified two cellular proteins, p87 and p120, that specifically bound to the stem-loop III structure. Mutations at the polypyrimidine tract sequence inhibited the binding of p87, but maintained the ability of the mutant HCV RNA to interact with p120. Translation competition assay demonstrated that the 5'NCR from nt 131 to 253 within the stem-loop III structure is important for the translation of HCV core protein. In addition, p120 and unidentified cellular proteins are likely to be involved in the translation of HCV polyprotein, whereas p87 may play important roles in HCV multiplication other than translation.

Authors+Show Affiliations

Institute of Biochemistry, College of Medicine, National Taiwan University, Taipei, Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7747444

Citation

Yen, J H., et al. "Cellular Proteins Specifically Bind to the 5'-noncoding Region of Hepatitis C Virus RNA." Virology, vol. 208, no. 2, 1995, pp. 723-32.
Yen JH, Chang SC, Hu CR, et al. Cellular proteins specifically bind to the 5'-noncoding region of hepatitis C virus RNA. Virology. 1995;208(2):723-32.
Yen, J. H., Chang, S. C., Hu, C. R., Chu, S. C., Lin, S. S., Hsieh, Y. S., & Chang, M. F. (1995). Cellular proteins specifically bind to the 5'-noncoding region of hepatitis C virus RNA. Virology, 208(2), 723-32.
Yen JH, et al. Cellular Proteins Specifically Bind to the 5'-noncoding Region of Hepatitis C Virus RNA. Virology. 1995 Apr 20;208(2):723-32. PubMed PMID: 7747444.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cellular proteins specifically bind to the 5'-noncoding region of hepatitis C virus RNA. AU - Yen,J H, AU - Chang,S C, AU - Hu,C R, AU - Chu,S C, AU - Lin,S S, AU - Hsieh,Y S, AU - Chang,M F, PY - 1995/4/20/pubmed PY - 1995/4/20/medline PY - 1995/4/20/entrez SP - 723 EP - 32 JF - Virology JO - Virology VL - 208 IS - 2 N2 - Hepatitis C virus (HCV) RNA contains a highly conserved 5'-noncoding region (5'NCR) which may be important in viral multiplication. To study the possible mechanisms of the cellular proteins involved in HCV replication and pathogenesis, a gel mobility shift assay and competition analysis were performed with the HCV 5'NCR. Two specific complexes were formed between the 341-nucleotide RNA of the HCV 5'NCR and proteins of mammalian cells. The specific RNA-protein complexes were maintained in the region of the 5'NCR from nucleotides 131 to 253. Nevertheless, the slower migrating RNA-protein complex failed to form when a polypyrimidine tract sequence (191-UCCUUUCUU-199) in the stem-loop III structure of HCV 5'NCR was changed to 191-UCCUUUggU-199. A uv cross-linking assay further identified two cellular proteins, p87 and p120, that specifically bound to the stem-loop III structure. Mutations at the polypyrimidine tract sequence inhibited the binding of p87, but maintained the ability of the mutant HCV RNA to interact with p120. Translation competition assay demonstrated that the 5'NCR from nt 131 to 253 within the stem-loop III structure is important for the translation of HCV core protein. In addition, p120 and unidentified cellular proteins are likely to be involved in the translation of HCV polyprotein, whereas p87 may play important roles in HCV multiplication other than translation. SN - 0042-6822 UR - https://www.unboundmedicine.com/medline/citation/7747444/Cellular_proteins_specifically_bind_to_the_5'_noncoding_region_of_hepatitis_C_virus_RNA_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0042-6822(85)71204-4 DB - PRIME DP - Unbound Medicine ER -