Tags

Type your tag names separated by a space and hit enter

Characterization of Dasypyrum villosum (L.) candargy chromosomal chromatin by means of in situ restriction endonucleases, fluorochromes, silver staining and C-banding.
Chromosome Res. 1995 Mar; 3(2):109-14.CR

Abstract

The distribution of C-banded heterochromatin was determined in an inbred line of Dasypyrum villosum. Practically no difference in chromosome morphology or band distribution could be observed within the chromosomes of the same pair. Heterochromatin bands, revealed by Giemsa banding, were characterized by means of their differential reaction to fluorochromes, silver staining and in situ digestion with different restriction endonucleases. The results clearly indicate that in D. villosum two different classes of heterochromatin with different chromosomal local-ization exist: one is evidenced by both C-banding and DAPI staining and has mainly telomeric distribution, the other is evidenced only by C-banding and has mainly centromeric distribution.

Authors+Show Affiliations

CNR Istituto del Germoplasma, Bari, Italy.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

7749559

Citation

Pignone, D, et al. "Characterization of Dasypyrum Villosum (L.) Candargy Chromosomal Chromatin By Means of in Situ Restriction Endonucleases, Fluorochromes, Silver Staining and C-banding." Chromosome Research : an International Journal On the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology, vol. 3, no. 2, 1995, pp. 109-14.
Pignone D, Galasso I, Rossino R, et al. Characterization of Dasypyrum villosum (L.) candargy chromosomal chromatin by means of in situ restriction endonucleases, fluorochromes, silver staining and C-banding. Chromosome Res. 1995;3(2):109-14.
Pignone, D., Galasso, I., Rossino, R., & Mezzanotte, R. (1995). Characterization of Dasypyrum villosum (L.) candargy chromosomal chromatin by means of in situ restriction endonucleases, fluorochromes, silver staining and C-banding. Chromosome Research : an International Journal On the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology, 3(2), 109-14.
Pignone D, et al. Characterization of Dasypyrum Villosum (L.) Candargy Chromosomal Chromatin By Means of in Situ Restriction Endonucleases, Fluorochromes, Silver Staining and C-banding. Chromosome Res. 1995;3(2):109-14. PubMed PMID: 7749559.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of Dasypyrum villosum (L.) candargy chromosomal chromatin by means of in situ restriction endonucleases, fluorochromes, silver staining and C-banding. AU - Pignone,D, AU - Galasso,I, AU - Rossino,R, AU - Mezzanotte,R, PY - 1995/3/1/pubmed PY - 1995/3/1/medline PY - 1995/3/1/entrez SP - 109 EP - 14 JF - Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology JO - Chromosome Res VL - 3 IS - 2 N2 - The distribution of C-banded heterochromatin was determined in an inbred line of Dasypyrum villosum. Practically no difference in chromosome morphology or band distribution could be observed within the chromosomes of the same pair. Heterochromatin bands, revealed by Giemsa banding, were characterized by means of their differential reaction to fluorochromes, silver staining and in situ digestion with different restriction endonucleases. The results clearly indicate that in D. villosum two different classes of heterochromatin with different chromosomal local-ization exist: one is evidenced by both C-banding and DAPI staining and has mainly telomeric distribution, the other is evidenced only by C-banding and has mainly centromeric distribution. SN - 0967-3849 UR - https://www.unboundmedicine.com/medline/citation/7749559/Characterization_of_Dasypyrum_villosum__L___candargy_chromosomal_chromatin_by_means_of_in_situ_restriction_endonucleases_fluorochromes_silver_staining_and_C_banding_ DB - PRIME DP - Unbound Medicine ER -