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Comparative evaluation of commercial Premier EIA and microimmunodiffusion and complement fixation tests for Coccidioides immitis antibodies.
J Clin Microbiol. 1995 Mar; 33(3):618-9.JC

Abstract

A total of 409 serum and cerebrospinal fluid specimens from human subjects with proven coccidioidomycosis, with other infections, or with no apparent illness were tested for antibodies to Coccidioides immitis by the Premier EIA (Meridian Diagnostics, Inc., Cincinnati, Ohio), which tests for immunoglobulin G (IgG) and IgM responses to coccidioidal antigens, and by the conventional complement fixation (CF) or immunodiffusion (ID) assays for antibodies corresponding to those detected by the tube precipitin (TP) or CF tests. Of the 409 specimens, 47 were from persons with confirmed coccidioidomycosis and all were positive for C. immitis antibodies in IDCF tests and enzyme immunoassays (EIAs) for both IgG and IgM. The EIA for detecting both IgG and IgM antibodies proved to be sensitive for detecting coccidioidomycosis case sera positive by the IDCF, IDTP, and CF tests. Maximal sensitivity for diagnosing coccidioidomycosis is dependent upon detection of both IgG and IgM antibodies in the EIA. The EIA, however, was not absolutely specific, since some sera from patients with confirmed blastomycosis and some from patients with noncoccidioidal disease produced false-positive reactions.

Authors+Show Affiliations

Division of Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

7751365

Citation

Kaufman, L, et al. "Comparative Evaluation of Commercial Premier EIA and Microimmunodiffusion and Complement Fixation Tests for Coccidioides Immitis Antibodies." Journal of Clinical Microbiology, vol. 33, no. 3, 1995, pp. 618-9.
Kaufman L, Sekhon AS, Moledina N, et al. Comparative evaluation of commercial Premier EIA and microimmunodiffusion and complement fixation tests for Coccidioides immitis antibodies. J Clin Microbiol. 1995;33(3):618-9.
Kaufman, L., Sekhon, A. S., Moledina, N., Jalbert, M., & Pappagianis, D. (1995). Comparative evaluation of commercial Premier EIA and microimmunodiffusion and complement fixation tests for Coccidioides immitis antibodies. Journal of Clinical Microbiology, 33(3), 618-9.
Kaufman L, et al. Comparative Evaluation of Commercial Premier EIA and Microimmunodiffusion and Complement Fixation Tests for Coccidioides Immitis Antibodies. J Clin Microbiol. 1995;33(3):618-9. PubMed PMID: 7751365.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative evaluation of commercial Premier EIA and microimmunodiffusion and complement fixation tests for Coccidioides immitis antibodies. AU - Kaufman,L, AU - Sekhon,A S, AU - Moledina,N, AU - Jalbert,M, AU - Pappagianis,D, PY - 1995/3/1/pubmed PY - 1995/3/1/medline PY - 1995/3/1/entrez SP - 618 EP - 9 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 33 IS - 3 N2 - A total of 409 serum and cerebrospinal fluid specimens from human subjects with proven coccidioidomycosis, with other infections, or with no apparent illness were tested for antibodies to Coccidioides immitis by the Premier EIA (Meridian Diagnostics, Inc., Cincinnati, Ohio), which tests for immunoglobulin G (IgG) and IgM responses to coccidioidal antigens, and by the conventional complement fixation (CF) or immunodiffusion (ID) assays for antibodies corresponding to those detected by the tube precipitin (TP) or CF tests. Of the 409 specimens, 47 were from persons with confirmed coccidioidomycosis and all were positive for C. immitis antibodies in IDCF tests and enzyme immunoassays (EIAs) for both IgG and IgM. The EIA for detecting both IgG and IgM antibodies proved to be sensitive for detecting coccidioidomycosis case sera positive by the IDCF, IDTP, and CF tests. Maximal sensitivity for diagnosing coccidioidomycosis is dependent upon detection of both IgG and IgM antibodies in the EIA. The EIA, however, was not absolutely specific, since some sera from patients with confirmed blastomycosis and some from patients with noncoccidioidal disease produced false-positive reactions. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/7751365/Comparative_evaluation_of_commercial_Premier_EIA_and_microimmunodiffusion_and_complement_fixation_tests_for_Coccidioides_immitis_antibodies_ L2 - https://journals.asm.org/doi/10.1128/jcm.33.3.618-619.1995?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -