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Glutamate induces the production of reactive oxygen species in cultured forebrain neurons following NMDA receptor activation.
J Neurosci. 1995 May; 15(5 Pt 1):3318-27.JN

Abstract

Using the fluorescent dye 2',7'-dichlorodihydrofluorescein (DCF-H2) we investigated the role of glutamate in the production of reactive oxygen species (ROS) in cultured neurons from fetal rat forebrain. The addition of an excitotoxic concentration of glutamate (100 microM) produced a generalized decrease in cellular DCF fluorescence accompanied by local areas of increased fluorescence around the margins of the cell body that could be observed within 2-4 min of glutamate exposure. Increases in fluorescence were dependent on NMDA receptor activation and Ca2+ entry and were blocked by the mitochondrial proton ionophore carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP). Additional studies suggested that the generalized decrease in fluorescence was due to intracellular acidification. These studies suggest a critical role for mitochondria in the production of ROS in association with glutamate excitotoxicity, and additionally demonstrate the feasibility of measuring the production of ROS at the level of the single cell.

Authors+Show Affiliations

Department of Pharmacology, University of Pittsburgh, Pennsylvania 15261, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7751912

Citation

Reynolds, I J., and T G. Hastings. "Glutamate Induces the Production of Reactive Oxygen Species in Cultured Forebrain Neurons Following NMDA Receptor Activation." The Journal of Neuroscience : the Official Journal of the Society for Neuroscience, vol. 15, no. 5 Pt 1, 1995, pp. 3318-27.
Reynolds IJ, Hastings TG. Glutamate induces the production of reactive oxygen species in cultured forebrain neurons following NMDA receptor activation. J Neurosci. 1995;15(5 Pt 1):3318-27.
Reynolds, I. J., & Hastings, T. G. (1995). Glutamate induces the production of reactive oxygen species in cultured forebrain neurons following NMDA receptor activation. The Journal of Neuroscience : the Official Journal of the Society for Neuroscience, 15(5 Pt 1), 3318-27.
Reynolds IJ, Hastings TG. Glutamate Induces the Production of Reactive Oxygen Species in Cultured Forebrain Neurons Following NMDA Receptor Activation. J Neurosci. 1995;15(5 Pt 1):3318-27. PubMed PMID: 7751912.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Glutamate induces the production of reactive oxygen species in cultured forebrain neurons following NMDA receptor activation. AU - Reynolds,I J, AU - Hastings,T G, PY - 1995/5/1/pubmed PY - 1995/5/1/medline PY - 1995/5/1/entrez SP - 3318 EP - 27 JF - The Journal of neuroscience : the official journal of the Society for Neuroscience JO - J Neurosci VL - 15 IS - 5 Pt 1 N2 - Using the fluorescent dye 2',7'-dichlorodihydrofluorescein (DCF-H2) we investigated the role of glutamate in the production of reactive oxygen species (ROS) in cultured neurons from fetal rat forebrain. The addition of an excitotoxic concentration of glutamate (100 microM) produced a generalized decrease in cellular DCF fluorescence accompanied by local areas of increased fluorescence around the margins of the cell body that could be observed within 2-4 min of glutamate exposure. Increases in fluorescence were dependent on NMDA receptor activation and Ca2+ entry and were blocked by the mitochondrial proton ionophore carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP). Additional studies suggested that the generalized decrease in fluorescence was due to intracellular acidification. These studies suggest a critical role for mitochondria in the production of ROS in association with glutamate excitotoxicity, and additionally demonstrate the feasibility of measuring the production of ROS at the level of the single cell. SN - 0270-6474 UR - https://www.unboundmedicine.com/medline/citation/7751912/Glutamate_induces_the_production_of_reactive_oxygen_species_in_cultured_forebrain_neurons_following_NMDA_receptor_activation_ L2 - http://www.jneurosci.org/cgi/pmidlookup?view=long&pmid=7751912 DB - PRIME DP - Unbound Medicine ER -