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Chiral separation of MDL 73,005EF enantiomers using an alpha 1-acid glycoprotein column.
J Chromatogr A. 1995 May 05; 699(1-2):389-94.JC

Abstract

The enantiomeric separation of MDL 73,005EF (racemic mixture of two enantiomers) has been accomplished using a Chiral AGP (alpha 1-acid glycoprotein) column. The enantiomers are baseline resolved with a runtime of less than 10 min. This separation is used to quantitate the enantiomers present in bulk drug samples, tabletted formulations, and drug used in pharmacological and toxicological studies. Variables found to have an effect on the enantiomeric separation were studied and include: mobile phase ionic strength, type and concentration of organic modifier added to the mobile phase, mobile phase, mobile phase pH, column temperature and the amount of analyte injected. The enantiomeric separation was optimized on the Chiral AGP column based on the effects that each variable had on the separation. Calibration curves for a standard were linear over a range of 0.24 to 61.2 micrograms/g with a correlation coefficient of better than 0.999. A detection limit of 0.012 micrograms/g and a quantitation limit of 0.24 micrograms/g were also found.

Authors+Show Affiliations

Pharmaceutical and Analytical Sciences, Marion Merrell Dow Inc., Kansas City, MO 64134, USA.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7757212

Citation

Bunton, B M., and T A. Walker. "Chiral Separation of MDL 73,005EF Enantiomers Using an Alpha 1-acid Glycoprotein Column." Journal of Chromatography. A, vol. 699, no. 1-2, 1995, pp. 389-94.
Bunton BM, Walker TA. Chiral separation of MDL 73,005EF enantiomers using an alpha 1-acid glycoprotein column. J Chromatogr A. 1995;699(1-2):389-94.
Bunton, B. M., & Walker, T. A. (1995). Chiral separation of MDL 73,005EF enantiomers using an alpha 1-acid glycoprotein column. Journal of Chromatography. A, 699(1-2), 389-94.
Bunton BM, Walker TA. Chiral Separation of MDL 73,005EF Enantiomers Using an Alpha 1-acid Glycoprotein Column. J Chromatogr A. 1995 May 5;699(1-2):389-94. PubMed PMID: 7757212.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chiral separation of MDL 73,005EF enantiomers using an alpha 1-acid glycoprotein column. AU - Bunton,B M, AU - Walker,T A, PY - 1995/5/5/pubmed PY - 1995/5/5/medline PY - 1995/5/5/entrez SP - 389 EP - 94 JF - Journal of chromatography. A JO - J Chromatogr A VL - 699 IS - 1-2 N2 - The enantiomeric separation of MDL 73,005EF (racemic mixture of two enantiomers) has been accomplished using a Chiral AGP (alpha 1-acid glycoprotein) column. The enantiomers are baseline resolved with a runtime of less than 10 min. This separation is used to quantitate the enantiomers present in bulk drug samples, tabletted formulations, and drug used in pharmacological and toxicological studies. Variables found to have an effect on the enantiomeric separation were studied and include: mobile phase ionic strength, type and concentration of organic modifier added to the mobile phase, mobile phase, mobile phase pH, column temperature and the amount of analyte injected. The enantiomeric separation was optimized on the Chiral AGP column based on the effects that each variable had on the separation. Calibration curves for a standard were linear over a range of 0.24 to 61.2 micrograms/g with a correlation coefficient of better than 0.999. A detection limit of 0.012 micrograms/g and a quantitation limit of 0.24 micrograms/g were also found. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/7757212/Chiral_separation_of_MDL_73005EF_enantiomers_using_an_alpha_1_acid_glycoprotein_column_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0021-9673(95)00088-5 DB - PRIME DP - Unbound Medicine ER -