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GABAA receptor subunits have differential distributions in the rat retina: in situ hybridization and immunohistochemistry.
J Comp Neurol 1995; 353(4):553-71JC

Abstract

The distributions of nine different subunits of the gamma-aminobutyric acidA (GABAA) receptor (alpha 1, alpha 2, alpha 3, alpha 5; beta 1, beta 2, beta 3; gamma 2; delta) were investigated in the rat retina using immunocytochemistry and in situ hybridization. With the exception of the alpha 5 subunit, all subunits could be localized. Each subunit was expressed in characteristic strata within the inner plexiform layer (IPL). Some subunits (e.g., gamma 2) showed a ubiquitous distribution, while others (e.g., delta) were restricted to narrow sublayers. Double labeling experiments using different combinations of the subunit-specific antibodies revealed colocalizations of subunits within individual neurons. Additionally, GABAA receptor subunits were mapped to distinct populations of retinal neurons by coapplication of defined immunocytochemical markers and subunit-specific antibodies. Cholinergic amacrine cells were found to express the alpha 2, beta 1, beta 2/3 and delta subunits, while dopaminergic amacrine cells express the alpha 2, alpha 3 and gamma 2 subunits. Dissociated rod bipolar cells express the alpha 1 and gamma 2 subunits. In summary, this study provides evidence for the existence of multiple GABAA receptor subtypes in the retina. The distinct stratification pattern of the subunits in the IPL suggests that different functional circuits involve specific subtypes of GABAA receptors.

Authors+Show Affiliations

Max-Planck-Institut für Hirnforschung, Frankfurt, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7759615

Citation

Greferath, U, et al. "GABAA Receptor Subunits Have Differential Distributions in the Rat Retina: in Situ Hybridization and Immunohistochemistry." The Journal of Comparative Neurology, vol. 353, no. 4, 1995, pp. 553-71.
Greferath U, Grünert U, Fritschy JM, et al. GABAA receptor subunits have differential distributions in the rat retina: in situ hybridization and immunohistochemistry. J Comp Neurol. 1995;353(4):553-71.
Greferath, U., Grünert, U., Fritschy, J. M., Stephenson, A., Möhler, H., & Wässle, H. (1995). GABAA receptor subunits have differential distributions in the rat retina: in situ hybridization and immunohistochemistry. The Journal of Comparative Neurology, 353(4), pp. 553-71.
Greferath U, et al. GABAA Receptor Subunits Have Differential Distributions in the Rat Retina: in Situ Hybridization and Immunohistochemistry. J Comp Neurol. 1995 Mar 20;353(4):553-71. PubMed PMID: 7759615.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - GABAA receptor subunits have differential distributions in the rat retina: in situ hybridization and immunohistochemistry. AU - Greferath,U, AU - Grünert,U, AU - Fritschy,J M, AU - Stephenson,A, AU - Möhler,H, AU - Wässle,H, PY - 1995/3/20/pubmed PY - 1995/3/20/medline PY - 1995/3/20/entrez SP - 553 EP - 71 JF - The Journal of comparative neurology JO - J. Comp. Neurol. VL - 353 IS - 4 N2 - The distributions of nine different subunits of the gamma-aminobutyric acidA (GABAA) receptor (alpha 1, alpha 2, alpha 3, alpha 5; beta 1, beta 2, beta 3; gamma 2; delta) were investigated in the rat retina using immunocytochemistry and in situ hybridization. With the exception of the alpha 5 subunit, all subunits could be localized. Each subunit was expressed in characteristic strata within the inner plexiform layer (IPL). Some subunits (e.g., gamma 2) showed a ubiquitous distribution, while others (e.g., delta) were restricted to narrow sublayers. Double labeling experiments using different combinations of the subunit-specific antibodies revealed colocalizations of subunits within individual neurons. Additionally, GABAA receptor subunits were mapped to distinct populations of retinal neurons by coapplication of defined immunocytochemical markers and subunit-specific antibodies. Cholinergic amacrine cells were found to express the alpha 2, beta 1, beta 2/3 and delta subunits, while dopaminergic amacrine cells express the alpha 2, alpha 3 and gamma 2 subunits. Dissociated rod bipolar cells express the alpha 1 and gamma 2 subunits. In summary, this study provides evidence for the existence of multiple GABAA receptor subtypes in the retina. The distinct stratification pattern of the subunits in the IPL suggests that different functional circuits involve specific subtypes of GABAA receptors. SN - 0021-9967 UR - https://www.unboundmedicine.com/medline/citation/7759615/GABAA_receptor_subunits_have_differential_distributions_in_the_rat_retina:_in_situ_hybridization_and_immunohistochemistry_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0021-9967&date=1995&volume=353&issue=4&spage=553 DB - PRIME DP - Unbound Medicine ER -