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The role of protein kinase in human synovial fibroblast growth.
Biochem Biophys Res Commun. 1995 May 25; 210(3):1066-75.BB

Abstract

The histological features of rheumatoid arthritis (RA) consist of overgrowth of synovial cells. Several growth factors that cause synovial hyperplasia have been identified in RA synovium. The basic-fibroblast growth factor (b-FGF), representing one of these growth factors, may play an important role in the pathogenesis of RA. We examined the b-FGF-mediated intracellular signal pathway involved in synovial cell growth. b-FGF-induced synovial cell growth was inhibited by protein tyrosine kinase (PTK) inhibitors, herbimycin A and genistein, but not by H7 that inhibits protein kinase C (PKC). Stimulation of synovial cells with b-FGF resulted in tyrosine phosphorylation of cellular proteins and MAP kinase activation. Our results also demonstrated that b-FGF-mediated activation of MAP kinase was inhibited by herbimycin A indicating that protein tyrosine kinase may be involved in the activation of MAP kinase in human synovial cells. However, inhibition of b-FGF-mediated MAP kinase activation by PKC downregulation did not occur.

Authors+Show Affiliations

First Department of Internal Medicine, Nagasaki University School of Medicine, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

7763235

Citation

Migita, K, et al. "The Role of Protein Kinase in Human Synovial Fibroblast Growth." Biochemical and Biophysical Research Communications, vol. 210, no. 3, 1995, pp. 1066-75.
Migita K, Eguchi K, Tsukada T, et al. The role of protein kinase in human synovial fibroblast growth. Biochem Biophys Res Commun. 1995;210(3):1066-75.
Migita, K., Eguchi, K., Tsukada, T., Kawabe, Y., Aoyagi, T., & Nagataki, S. (1995). The role of protein kinase in human synovial fibroblast growth. Biochemical and Biophysical Research Communications, 210(3), 1066-75.
Migita K, et al. The Role of Protein Kinase in Human Synovial Fibroblast Growth. Biochem Biophys Res Commun. 1995 May 25;210(3):1066-75. PubMed PMID: 7763235.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The role of protein kinase in human synovial fibroblast growth. AU - Migita,K, AU - Eguchi,K, AU - Tsukada,T, AU - Kawabe,Y, AU - Aoyagi,T, AU - Nagataki,S, PY - 1995/5/25/pubmed PY - 1995/5/25/medline PY - 1995/5/25/entrez SP - 1066 EP - 75 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 210 IS - 3 N2 - The histological features of rheumatoid arthritis (RA) consist of overgrowth of synovial cells. Several growth factors that cause synovial hyperplasia have been identified in RA synovium. The basic-fibroblast growth factor (b-FGF), representing one of these growth factors, may play an important role in the pathogenesis of RA. We examined the b-FGF-mediated intracellular signal pathway involved in synovial cell growth. b-FGF-induced synovial cell growth was inhibited by protein tyrosine kinase (PTK) inhibitors, herbimycin A and genistein, but not by H7 that inhibits protein kinase C (PKC). Stimulation of synovial cells with b-FGF resulted in tyrosine phosphorylation of cellular proteins and MAP kinase activation. Our results also demonstrated that b-FGF-mediated activation of MAP kinase was inhibited by herbimycin A indicating that protein tyrosine kinase may be involved in the activation of MAP kinase in human synovial cells. However, inhibition of b-FGF-mediated MAP kinase activation by PKC downregulation did not occur. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/7763235/The_role_of_protein_kinase_in_human_synovial_fibroblast_growth_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(85)71765-2 DB - PRIME DP - Unbound Medicine ER -