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Targeting of oleosins to the oil bodies of oilseed rape (Brassica napus L.).
Planta. 1993 Jan; 189(1):24-9.P

Abstract

Oleosins of Brassica napus L. (oilseed rape) synthesized by in-vitro translation were found to be very efficiently targeted to microsomal membranes but only poorly translocated to oil bodies or emulsified oil. The use of other bilayer membranes as controls showed that this interaction was specific. The rate of oleosin synthesis in the presence of microsomes was enhanced about threefold, indicative of the involvement of the signal-recognition particle in the targeting process. There is no evidence for the cleavage of the protein during targeting and the protein sequence reveals no consensus cleavage site for the signal peptide. Protection experiments using Proteinase K revealed that about 6 kDa of the protein is exposed on the cytoplasmic side of the ER but the remainder is protected. Carbonate (pH 11) washing of microsomal membranes after in-vitro translation confirmed that oleosins have a domain which remains inserted in the ER rather than the protein being transported completely into the lumen of the ER. These results indicate that oleosins are transported via the ER prior to their accumulation on oil bodies.

Authors+Show Affiliations

Department of Brassica and Oilseeds Research, John Innes Centre, Norwich, UK.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7763356

Citation

Hills, M J., et al. "Targeting of Oleosins to the Oil Bodies of Oilseed Rape (Brassica Napus L.)." Planta, vol. 189, no. 1, 1993, pp. 24-9.
Hills MJ, Watson MD, Murphy DJ. Targeting of oleosins to the oil bodies of oilseed rape (Brassica napus L.). Planta. 1993;189(1):24-9.
Hills, M. J., Watson, M. D., & Murphy, D. J. (1993). Targeting of oleosins to the oil bodies of oilseed rape (Brassica napus L.). Planta, 189(1), 24-9.
Hills MJ, Watson MD, Murphy DJ. Targeting of Oleosins to the Oil Bodies of Oilseed Rape (Brassica Napus L.). Planta. 1993;189(1):24-9. PubMed PMID: 7763356.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Targeting of oleosins to the oil bodies of oilseed rape (Brassica napus L.). AU - Hills,M J, AU - Watson,M D, AU - Murphy,D J, PY - 1993/1/1/pubmed PY - 1993/1/1/medline PY - 1993/1/1/entrez SP - 24 EP - 9 JF - Planta JO - Planta VL - 189 IS - 1 N2 - Oleosins of Brassica napus L. (oilseed rape) synthesized by in-vitro translation were found to be very efficiently targeted to microsomal membranes but only poorly translocated to oil bodies or emulsified oil. The use of other bilayer membranes as controls showed that this interaction was specific. The rate of oleosin synthesis in the presence of microsomes was enhanced about threefold, indicative of the involvement of the signal-recognition particle in the targeting process. There is no evidence for the cleavage of the protein during targeting and the protein sequence reveals no consensus cleavage site for the signal peptide. Protection experiments using Proteinase K revealed that about 6 kDa of the protein is exposed on the cytoplasmic side of the ER but the remainder is protected. Carbonate (pH 11) washing of microsomal membranes after in-vitro translation confirmed that oleosins have a domain which remains inserted in the ER rather than the protein being transported completely into the lumen of the ER. These results indicate that oleosins are transported via the ER prior to their accumulation on oil bodies. SN - 0032-0935 UR - https://www.unboundmedicine.com/medline/citation/7763356/Targeting_of_oleosins_to_the_oil_bodies_of_oilseed_rape__Brassica_napus_L___ DB - PRIME DP - Unbound Medicine ER -