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Antitumor activities and immunochemical properties of the cell-wall polysaccharides from Aureobasidium pullulans.
Biosci Biotechnol Biochem. 1994 Dec; 58(12):2145-51.BB

Abstract

Delipidated cell walls from Aureobasidium pullulans were fractionated systematically. The cell surface heteropolysaccharide contains D-mannose, D-galactose, D-glucose, and D-glucuronic acid (ratio, 8.5:3.9:1.0:1.0). It consists of a backbone of (1-->6)-alpha-linked D-mannose residues, some of which are substituted at 0-3 with single or beta-(1-->6)-linked D-galactofuranosyl side chains, some terminated with a D-glucuronic acid residue, and also with single residues of D-glucopyranose, D-galactopyranose, and D-mannopyranose. This glucurono-gluco-galactomannan interacted with antiserum against Elsinoe leucospila, which also reacted with its galactomannan, indicating that both polysaccharides contain a common epitope, i.e., at least terminal beta-galactofuranosyl groups and also possibly internal beta-(1-->6)-linked galactofuranose residues. It was further separated by DEAE-Sephacel column chromatography to gluco-galactomannan and glucurono-gluco-galactomannan. The alkali-extracted beta-D-glucan was purified by DEAE-cellulose chromatography to afford two antitumor-active (1-->3)-beta-D-glucans. One of the glucans (M(r), 1-2 x 10(5)) was a O-6-branched (1-->3)-beta-D-glucan with a single beta-D-glucosyl residue, d.b., 1/7, and the other (M(r), 3.5-4.5 x 10(5)) had similar branched structure, but having d.b., 1/5. Side chains of both glucans contain small proportions of beta-(1-->6)- and beta-(1-->4)-D-glucosidic linkages.

Links

Publisher Full Text
joi.jlc.jst.go.jp
tandfonline.com

Authors+Show Affiliations

Kataoka-Shirasugi N
Department of Food and Nutrition, Faculty of Human Life Science, Osaka City University, Japan.
Ikuta J
No affiliation info available
Kuroshima A
No affiliation info available
Misaki A
No affiliation info available

MeSH

Antineoplastic AgentsCarbohydrate SequenceCell WallChromatography, Ion ExchangeImmunochemistryMitosporic FungiMolecular Sequence DataPolysaccharides

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7765709

Citation

Kataoka-Shirasugi, N, et al. "Antitumor Activities and Immunochemical Properties of the Cell-wall Polysaccharides From Aureobasidium Pullulans." Bioscience, Biotechnology, and Biochemistry, vol. 58, no. 12, 1994, pp. 2145-51.
Kataoka-Shirasugi N, Ikuta J, Kuroshima A, et al. Antitumor activities and immunochemical properties of the cell-wall polysaccharides from Aureobasidium pullulans. Biosci Biotechnol Biochem. 1994;58(12):2145-51.
Kataoka-Shirasugi, N., Ikuta, J., Kuroshima, A., & Misaki, A. (1994). Antitumor activities and immunochemical properties of the cell-wall polysaccharides from Aureobasidium pullulans. Bioscience, Biotechnology, and Biochemistry, 58(12), 2145-51.
Kataoka-Shirasugi N, et al. Antitumor Activities and Immunochemical Properties of the Cell-wall Polysaccharides From Aureobasidium Pullulans. Biosci Biotechnol Biochem. 1994;58(12):2145-51. PubMed PMID: 7765709.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antitumor activities and immunochemical properties of the cell-wall polysaccharides from Aureobasidium pullulans. AU - Kataoka-Shirasugi,N, AU - Ikuta,J, AU - Kuroshima,A, AU - Misaki,A, PY - 1994/12/1/pubmed PY - 1994/12/1/medline PY - 1994/12/1/entrez SP - 2145 EP - 51 JF - Bioscience, biotechnology, and biochemistry JO - Biosci Biotechnol Biochem VL - 58 IS - 12 N2 - Delipidated cell walls from Aureobasidium pullulans were fractionated systematically. The cell surface heteropolysaccharide contains D-mannose, D-galactose, D-glucose, and D-glucuronic acid (ratio, 8.5:3.9:1.0:1.0). It consists of a backbone of (1-->6)-alpha-linked D-mannose residues, some of which are substituted at 0-3 with single or beta-(1-->6)-linked D-galactofuranosyl side chains, some terminated with a D-glucuronic acid residue, and also with single residues of D-glucopyranose, D-galactopyranose, and D-mannopyranose. This glucurono-gluco-galactomannan interacted with antiserum against Elsinoe leucospila, which also reacted with its galactomannan, indicating that both polysaccharides contain a common epitope, i.e., at least terminal beta-galactofuranosyl groups and also possibly internal beta-(1-->6)-linked galactofuranose residues. It was further separated by DEAE-Sephacel column chromatography to gluco-galactomannan and glucurono-gluco-galactomannan. The alkali-extracted beta-D-glucan was purified by DEAE-cellulose chromatography to afford two antitumor-active (1-->3)-beta-D-glucans. One of the glucans (M(r), 1-2 x 10(5)) was a O-6-branched (1-->3)-beta-D-glucan with a single beta-D-glucosyl residue, d.b., 1/7, and the other (M(r), 3.5-4.5 x 10(5)) had similar branched structure, but having d.b., 1/5. Side chains of both glucans contain small proportions of beta-(1-->6)- and beta-(1-->4)-D-glucosidic linkages. SN - 0916-8451 UR - https://www.unboundmedicine.com/medline/citation/7765709/Antitumor_activities_and_immunochemical_properties_of_the_cell_wall_polysaccharides_from_Aureobasidium_pullulans_ L2 - https://joi.jlc.jst.go.jp/JST.Journalarchive/bbb1992/58.2145?lang=en&from=PubMed DB - PRIME DP - Unbound Medicine ER -