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Effects of allergen challenge on eosinophils, eosinophil cationic protein, and granulocyte-macrophage colony-stimulating factor in mild asthma.
Am J Respir Crit Care Med. 1995 Jun; 151(6):1915-24.AJ

Abstract

Allergen inhalation challenge is associated with increases in eosinophil number and activation, and provides a useful model for investigating airway inflammation in asthma. Limited information, however, is available on the effect of allergen challenge on cytokines regulating eosinophil function. We investigated allergen-induced changes in eosinophil number and activation and in granulocyte-macrophage colony-stimulating factor (GM-CSF), a cytokine known to regulate eosinophil function in vitro. Seven subjects with mild atopic asthma and late asthmatic responses completed diluent- and allergen-inhalation challenges. Blood, bronchoalveolar lavage fluid (BALF), and biopsy samples were collected 24 h after challenge. Allergen inhalation caused a significant increase in eosinophils in BALF and biopsy samples. Eosinophil activation, as assessed by secretion of eosinophil cationic protein, and GM-CSF levels were significantly increased in BALF and bronchoalveolar lavage (BAL) cells. Allergen inhalation did not cause a significant change in eosinophil activation in biopsy tissue but did result in a significant decrease in GM-CSF in the tissue. Significant correlations were shown between the concentration of GM-CSF in BALF and the percentage of BAL eosinophils (Rs = 0.75, p = 0.05), severity of the late asthmatic response, and number of BAL eosinophils (Rs = 0.82, p = 0.02). A trend was seen between the late response and the concentration of GM-CSF in BALF. These results are consistent with the hypothesis that eosinophils, regulated by GM-CSF, contribute to allergen-induced decreases in airway function.

Authors+Show Affiliations

Department of Medicine, McMaster University, Hamilton, Ontario, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7767540

Citation

Woolley, K L., et al. "Effects of Allergen Challenge On Eosinophils, Eosinophil Cationic Protein, and Granulocyte-macrophage Colony-stimulating Factor in Mild Asthma." American Journal of Respiratory and Critical Care Medicine, vol. 151, no. 6, 1995, pp. 1915-24.
Woolley KL, Adelroth E, Woolley MJ, et al. Effects of allergen challenge on eosinophils, eosinophil cationic protein, and granulocyte-macrophage colony-stimulating factor in mild asthma. Am J Respir Crit Care Med. 1995;151(6):1915-24.
Woolley, K. L., Adelroth, E., Woolley, M. J., Ellis, R., Jordana, M., & O'Byrne, P. M. (1995). Effects of allergen challenge on eosinophils, eosinophil cationic protein, and granulocyte-macrophage colony-stimulating factor in mild asthma. American Journal of Respiratory and Critical Care Medicine, 151(6), 1915-24.
Woolley KL, et al. Effects of Allergen Challenge On Eosinophils, Eosinophil Cationic Protein, and Granulocyte-macrophage Colony-stimulating Factor in Mild Asthma. Am J Respir Crit Care Med. 1995;151(6):1915-24. PubMed PMID: 7767540.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of allergen challenge on eosinophils, eosinophil cationic protein, and granulocyte-macrophage colony-stimulating factor in mild asthma. AU - Woolley,K L, AU - Adelroth,E, AU - Woolley,M J, AU - Ellis,R, AU - Jordana,M, AU - O'Byrne,P M, PY - 1995/6/1/pubmed PY - 1995/6/1/medline PY - 1995/6/1/entrez SP - 1915 EP - 24 JF - American journal of respiratory and critical care medicine JO - Am J Respir Crit Care Med VL - 151 IS - 6 N2 - Allergen inhalation challenge is associated with increases in eosinophil number and activation, and provides a useful model for investigating airway inflammation in asthma. Limited information, however, is available on the effect of allergen challenge on cytokines regulating eosinophil function. We investigated allergen-induced changes in eosinophil number and activation and in granulocyte-macrophage colony-stimulating factor (GM-CSF), a cytokine known to regulate eosinophil function in vitro. Seven subjects with mild atopic asthma and late asthmatic responses completed diluent- and allergen-inhalation challenges. Blood, bronchoalveolar lavage fluid (BALF), and biopsy samples were collected 24 h after challenge. Allergen inhalation caused a significant increase in eosinophils in BALF and biopsy samples. Eosinophil activation, as assessed by secretion of eosinophil cationic protein, and GM-CSF levels were significantly increased in BALF and bronchoalveolar lavage (BAL) cells. Allergen inhalation did not cause a significant change in eosinophil activation in biopsy tissue but did result in a significant decrease in GM-CSF in the tissue. Significant correlations were shown between the concentration of GM-CSF in BALF and the percentage of BAL eosinophils (Rs = 0.75, p = 0.05), severity of the late asthmatic response, and number of BAL eosinophils (Rs = 0.82, p = 0.02). A trend was seen between the late response and the concentration of GM-CSF in BALF. These results are consistent with the hypothesis that eosinophils, regulated by GM-CSF, contribute to allergen-induced decreases in airway function. SN - 1073-449X UR - https://www.unboundmedicine.com/medline/citation/7767540/Effects_of_allergen_challenge_on_eosinophils_eosinophil_cationic_protein_and_granulocyte_macrophage_colony_stimulating_factor_in_mild_asthma_ L2 - https://www.atsjournals.org/doi/10.1164/ajrccm.151.6.7767540?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -