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Capillary gas chromatography with four different detectors for dinitroaniline herbicides in human body fluids.
J Anal Toxicol. 1995 Mar-Apr; 19(2):95-8.JA

Abstract

A simple and rapid method for extraction of seven dinitroaniline herbicides from human whole blood and urine using Sep-Pak C18 cartridges is presented. After mixing with distilled water, whole blood and urine samples containing the herbicides were loaded on Sep-Pak C18 cartridges and eluted with chloroform-methanol (9:1). The recovery of the seven compounds that were added to whole blood and urine was more than 91%. The herbicides were detected by capillary gas chromatography (GC) with electron-capture detection (ECD), surface-ionization detection (SID), nitrogen-phosphorus detection (NPD), and flame-ionization detection (FID). GC-ECD generally gave the highest sensitivity for all herbicides, whereas GC-FID gave the lowest sensitivity. Isopropalin could be detected by GC-SID with a sensitivity comparable with that of GC-ECD; high sensitivity could also be obtained for isopropalin, pendimethalin, and nitralin using GC-NPD. The calibration curve for the seven herbicides measured by GC-ECD showed linearity in the range of 0.047-3.0 pmol on-column. The detection limits of the herbicides were 24-45 fmol on-column for whole blood and 19-40 fmol on-column for urine.

Authors+Show Affiliations

Department of Legal Medicine, Showa University School of Medicine, Tokyo, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7769795

Citation

Kumazawa, T, et al. "Capillary Gas Chromatography With Four Different Detectors for Dinitroaniline Herbicides in Human Body Fluids." Journal of Analytical Toxicology, vol. 19, no. 2, 1995, pp. 95-8.
Kumazawa T, Sato K, Seno H, et al. Capillary gas chromatography with four different detectors for dinitroaniline herbicides in human body fluids. J Anal Toxicol. 1995;19(2):95-8.
Kumazawa, T., Sato, K., Seno, H., Ishii, A., & Suzuki, O. (1995). Capillary gas chromatography with four different detectors for dinitroaniline herbicides in human body fluids. Journal of Analytical Toxicology, 19(2), 95-8.
Kumazawa T, et al. Capillary Gas Chromatography With Four Different Detectors for Dinitroaniline Herbicides in Human Body Fluids. J Anal Toxicol. 1995 Mar-Apr;19(2):95-8. PubMed PMID: 7769795.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Capillary gas chromatography with four different detectors for dinitroaniline herbicides in human body fluids. AU - Kumazawa,T, AU - Sato,K, AU - Seno,H, AU - Ishii,A, AU - Suzuki,O, PY - 1995/3/1/pubmed PY - 1995/3/1/medline PY - 1995/3/1/entrez SP - 95 EP - 8 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 19 IS - 2 N2 - A simple and rapid method for extraction of seven dinitroaniline herbicides from human whole blood and urine using Sep-Pak C18 cartridges is presented. After mixing with distilled water, whole blood and urine samples containing the herbicides were loaded on Sep-Pak C18 cartridges and eluted with chloroform-methanol (9:1). The recovery of the seven compounds that were added to whole blood and urine was more than 91%. The herbicides were detected by capillary gas chromatography (GC) with electron-capture detection (ECD), surface-ionization detection (SID), nitrogen-phosphorus detection (NPD), and flame-ionization detection (FID). GC-ECD generally gave the highest sensitivity for all herbicides, whereas GC-FID gave the lowest sensitivity. Isopropalin could be detected by GC-SID with a sensitivity comparable with that of GC-ECD; high sensitivity could also be obtained for isopropalin, pendimethalin, and nitralin using GC-NPD. The calibration curve for the seven herbicides measured by GC-ECD showed linearity in the range of 0.047-3.0 pmol on-column. The detection limits of the herbicides were 24-45 fmol on-column for whole blood and 19-40 fmol on-column for urine. SN - 0146-4760 UR - https://www.unboundmedicine.com/medline/citation/7769795/Capillary_gas_chromatography_with_four_different_detectors_for_dinitroaniline_herbicides_in_human_body_fluids_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/jat/19.2.95 DB - PRIME DP - Unbound Medicine ER -