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Selective T cell depletion with CD8-conjugated magnetic beads in the prevention of graft-versus-host disease after allogeneic bone marrow transplantation.
Bone Marrow Transplant. 1995 Feb; 15(2):271-8.BM

Abstract

The effects of a new immunomagnetic method of selectively depleting CD8+ lymphocytes from donor bone marrow were studied in 29 patients undergoing transplantation from HLA-identical sibling (n = 20) or alternative (n = 9) donors. The direct immunomagnetic depletion method consistently removed > 95% of CD8+ cells and the non-specific loss of other cell subsets was only about 15%. Recovery of CFU-GM and BFU-e was on average > 100%. The final graft contained 0.9 +/- 0.6 x 10(8)/kg nucleated cells and 1.4 +/- 2.7 x 10(5)/kg CD8+ cells. Patients also received cyclosporine starting day -1. Engraftment occurred in 28 patients (97%), including three patients who received a non-TBI conditioning regimen. One patient receiving an unrelated transplant failed to engraft. Median time to ANC > 500 x 10(6)/L was 17 (12-23) days. Four of 20 patients receiving grafts from HLA-identical siblings (20%) developed acute GVHD grade > or = II. However, five of eight patients with grafts from alternative donors (63%) had grade > or = II GVHD. Nearly all patients developed fever around day 7, accompanied by fluid overload, mild skin rash and shortness of breath. This syndrome necessitated treatment with steroids. Immunomagnetic CD8 depletion is a simple and reproducible method of selective T cell depletion. In combination with cyclosporine it appears to be effective in the prevention of severe acute GVHD in HLA-identical sibling transplants, but not in transplants from less perfectly matched donors.

Authors+Show Affiliations

Bone Marrow Transplantation Program, Methodist Hospital of Indiana, Indianapolis 46206-1367, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7773217

Citation

Jansen, J, et al. "Selective T Cell Depletion With CD8-conjugated Magnetic Beads in the Prevention of Graft-versus-host Disease After Allogeneic Bone Marrow Transplantation." Bone Marrow Transplantation, vol. 15, no. 2, 1995, pp. 271-8.
Jansen J, Hanks S, Akard L, et al. Selective T cell depletion with CD8-conjugated magnetic beads in the prevention of graft-versus-host disease after allogeneic bone marrow transplantation. Bone Marrow Transplant. 1995;15(2):271-8.
Jansen, J., Hanks, S., Akard, L., Martin, M., Thompson, J., Chang, Q., Ash, R., Garrett, P., Figg, F., & English, D. (1995). Selective T cell depletion with CD8-conjugated magnetic beads in the prevention of graft-versus-host disease after allogeneic bone marrow transplantation. Bone Marrow Transplantation, 15(2), 271-8.
Jansen J, et al. Selective T Cell Depletion With CD8-conjugated Magnetic Beads in the Prevention of Graft-versus-host Disease After Allogeneic Bone Marrow Transplantation. Bone Marrow Transplant. 1995;15(2):271-8. PubMed PMID: 7773217.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selective T cell depletion with CD8-conjugated magnetic beads in the prevention of graft-versus-host disease after allogeneic bone marrow transplantation. AU - Jansen,J, AU - Hanks,S, AU - Akard,L, AU - Martin,M, AU - Thompson,J, AU - Chang,Q, AU - Ash,R, AU - Garrett,P, AU - Figg,F, AU - English,D, PY - 1995/2/1/pubmed PY - 1995/2/1/medline PY - 1995/2/1/entrez SP - 271 EP - 8 JF - Bone marrow transplantation JO - Bone Marrow Transplant VL - 15 IS - 2 N2 - The effects of a new immunomagnetic method of selectively depleting CD8+ lymphocytes from donor bone marrow were studied in 29 patients undergoing transplantation from HLA-identical sibling (n = 20) or alternative (n = 9) donors. The direct immunomagnetic depletion method consistently removed > 95% of CD8+ cells and the non-specific loss of other cell subsets was only about 15%. Recovery of CFU-GM and BFU-e was on average > 100%. The final graft contained 0.9 +/- 0.6 x 10(8)/kg nucleated cells and 1.4 +/- 2.7 x 10(5)/kg CD8+ cells. Patients also received cyclosporine starting day -1. Engraftment occurred in 28 patients (97%), including three patients who received a non-TBI conditioning regimen. One patient receiving an unrelated transplant failed to engraft. Median time to ANC > 500 x 10(6)/L was 17 (12-23) days. Four of 20 patients receiving grafts from HLA-identical siblings (20%) developed acute GVHD grade > or = II. However, five of eight patients with grafts from alternative donors (63%) had grade > or = II GVHD. Nearly all patients developed fever around day 7, accompanied by fluid overload, mild skin rash and shortness of breath. This syndrome necessitated treatment with steroids. Immunomagnetic CD8 depletion is a simple and reproducible method of selective T cell depletion. In combination with cyclosporine it appears to be effective in the prevention of severe acute GVHD in HLA-identical sibling transplants, but not in transplants from less perfectly matched donors. SN - 0268-3369 UR - https://www.unboundmedicine.com/medline/citation/7773217/Selective_T_cell_depletion_with_CD8_conjugated_magnetic_beads_in_the_prevention_of_graft_versus_host_disease_after_allogeneic_bone_marrow_transplantation_ L2 - http://www.diseaseinfosearch.org/result/7171 DB - PRIME DP - Unbound Medicine ER -