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Presence of specific platelet-activating factor binding-sites in neuroblastoma N1E-115 cells.
Neurosci Lett. 1995 Feb 17; 186(2-3):173-6.NL

Abstract

In this study we reported evidence for the existence of specific binding sites for platelet-activating factor (PAF) in neuroblastoma N1E-115 cells. The specific [3H]PAF binding reached a steady state level within 60 min at 25 degrees C. Scatchard analysis of the specific [3H]PAF binding revealed the presence of two apparent populations of binding sites. The high-affinity binding site possessed a Kd1 of 2.5 +/- 0.6 pM and Bmax1 = 57.3 +/- 20.0 fmol/mg protein. The low-affinity binding site possessed a Kd2 = 3.2 +/- 1.0 nM and Bmax2 = 4.4 +/- 2.1 pmol/mg protein. Furthermore, the total [3H]PAF binding was partially displaced by unlabelled PAF, PAF antagonists BN 52021 and BN 50730 in a dose-dependent manner. This study confirms the presence of specific PAF receptors in neuronal cells.

Authors+Show Affiliations

Unité de Radioprotection Centre de Recherches du Service de Santé des Armées Emile Pardé 24, La Tronche, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7777190

Citation

Lalouette, F, et al. "Presence of Specific Platelet-activating Factor Binding-sites in Neuroblastoma N1E-115 Cells." Neuroscience Letters, vol. 186, no. 2-3, 1995, pp. 173-6.
Lalouette F, Diserbo M, Martin C, et al. Presence of specific platelet-activating factor binding-sites in neuroblastoma N1E-115 cells. Neurosci Lett. 1995;186(2-3):173-6.
Lalouette, F., Diserbo, M., Martin, C., Verdetti, J., & Fatome, M. (1995). Presence of specific platelet-activating factor binding-sites in neuroblastoma N1E-115 cells. Neuroscience Letters, 186(2-3), 173-6.
Lalouette F, et al. Presence of Specific Platelet-activating Factor Binding-sites in Neuroblastoma N1E-115 Cells. Neurosci Lett. 1995 Feb 17;186(2-3):173-6. PubMed PMID: 7777190.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Presence of specific platelet-activating factor binding-sites in neuroblastoma N1E-115 cells. AU - Lalouette,F, AU - Diserbo,M, AU - Martin,C, AU - Verdetti,J, AU - Fatome,M, PY - 1995/2/17/pubmed PY - 1995/2/17/medline PY - 1995/2/17/entrez SP - 173 EP - 6 JF - Neuroscience letters JO - Neurosci Lett VL - 186 IS - 2-3 N2 - In this study we reported evidence for the existence of specific binding sites for platelet-activating factor (PAF) in neuroblastoma N1E-115 cells. The specific [3H]PAF binding reached a steady state level within 60 min at 25 degrees C. Scatchard analysis of the specific [3H]PAF binding revealed the presence of two apparent populations of binding sites. The high-affinity binding site possessed a Kd1 of 2.5 +/- 0.6 pM and Bmax1 = 57.3 +/- 20.0 fmol/mg protein. The low-affinity binding site possessed a Kd2 = 3.2 +/- 1.0 nM and Bmax2 = 4.4 +/- 2.1 pmol/mg protein. Furthermore, the total [3H]PAF binding was partially displaced by unlabelled PAF, PAF antagonists BN 52021 and BN 50730 in a dose-dependent manner. This study confirms the presence of specific PAF receptors in neuronal cells. SN - 0304-3940 UR - https://www.unboundmedicine.com/medline/citation/7777190/Presence_of_specific_platelet_activating_factor_binding_sites_in_neuroblastoma_N1E_115_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0304-3940(95)11319-R DB - PRIME DP - Unbound Medicine ER -