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Fertilization and pregnancy with acrosome intact sperm by intracytoplasmic sperm injection in patients with disordered zona pellucida-induced acrosome reaction.
Fertil Steril 1995; 64(1):116-21FS

Abstract

OBJECTIVE

To determine whether fertilization and pregnancy could be obtained by injection of acrosome-intact sperm into the cytoplasm in patients with persistent failure of fertilization in IVF-ET associated with disordered zona pellucida (ZP)-induced acrosome reaction (AR).

DESIGN

Sperm-ZP binding and penetration and ZP-induced AR were compared between patients and fertile donors. Acrosome-intact sperm removed from the ZP were injected into the cytoplasm of the oocytes.

SETTING

Reproductive Biology Unit, Royal Women's Hospital.

MAIN OUTCOME MEASURES

The proportion of ZP penetrated and ZP-induced AR and fertilization and pregnancy after intracytoplasmic sperm injection were analyzed.

RESULTS

Most (7/8) of the patients had consistently normal sperm characteristics including concentration, motility and velocity, acrosomes, and morphology. Mean number of sperm bound to the ZP was not significantly different between patients (97 sperm/ZP) and fertile donor controls (100 sperm/ZP). However, AR of sperm bound to the ZP was significantly lower in the patients (4%) than in controls (61%). None of the ZP (n = 32) were penetrated by patient sperm whereas all (n = 32) ZP were penetrated by control sperm. Acrosome intact sperm removed from the ZP were used for intracytoplasmic sperm injection. All patients had > or = 50% normal fertilization and embryo development. Three pregnancies (one early aborted, two ongoing) were achieved after the transfer of fresh or frozen embryos.

CONCLUSION

Acrosome-intact human sperm can produce a high fertilization rate and pregnancies after intracytoplasmic sperm injection in patients with disordered ZP-induced AR. The acrosome reaction is unlikely to be important for fertilization with intracytoplasmic sperm injection.

Authors+Show Affiliations

Department of Obstetrics and Gynaecology, Royal Women's Hospital, University of Melbourne, Carlton, Victoria, Australia.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7789545

Citation

Liu, D Y., et al. "Fertilization and Pregnancy With Acrosome Intact Sperm By Intracytoplasmic Sperm Injection in Patients With Disordered Zona Pellucida-induced Acrosome Reaction." Fertility and Sterility, vol. 64, no. 1, 1995, pp. 116-21.
Liu DY, Bourne H, Baker HW. Fertilization and pregnancy with acrosome intact sperm by intracytoplasmic sperm injection in patients with disordered zona pellucida-induced acrosome reaction. Fertil Steril. 1995;64(1):116-21.
Liu, D. Y., Bourne, H., & Baker, H. W. (1995). Fertilization and pregnancy with acrosome intact sperm by intracytoplasmic sperm injection in patients with disordered zona pellucida-induced acrosome reaction. Fertility and Sterility, 64(1), pp. 116-21.
Liu DY, Bourne H, Baker HW. Fertilization and Pregnancy With Acrosome Intact Sperm By Intracytoplasmic Sperm Injection in Patients With Disordered Zona Pellucida-induced Acrosome Reaction. Fertil Steril. 1995;64(1):116-21. PubMed PMID: 7789545.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fertilization and pregnancy with acrosome intact sperm by intracytoplasmic sperm injection in patients with disordered zona pellucida-induced acrosome reaction. AU - Liu,D Y, AU - Bourne,H, AU - Baker,H W, PY - 1995/7/1/pubmed PY - 1995/7/1/medline PY - 1995/7/1/entrez SP - 116 EP - 21 JF - Fertility and sterility JO - Fertil. Steril. VL - 64 IS - 1 N2 - OBJECTIVE: To determine whether fertilization and pregnancy could be obtained by injection of acrosome-intact sperm into the cytoplasm in patients with persistent failure of fertilization in IVF-ET associated with disordered zona pellucida (ZP)-induced acrosome reaction (AR). DESIGN: Sperm-ZP binding and penetration and ZP-induced AR were compared between patients and fertile donors. Acrosome-intact sperm removed from the ZP were injected into the cytoplasm of the oocytes. SETTING: Reproductive Biology Unit, Royal Women's Hospital. MAIN OUTCOME MEASURES: The proportion of ZP penetrated and ZP-induced AR and fertilization and pregnancy after intracytoplasmic sperm injection were analyzed. RESULTS: Most (7/8) of the patients had consistently normal sperm characteristics including concentration, motility and velocity, acrosomes, and morphology. Mean number of sperm bound to the ZP was not significantly different between patients (97 sperm/ZP) and fertile donor controls (100 sperm/ZP). However, AR of sperm bound to the ZP was significantly lower in the patients (4%) than in controls (61%). None of the ZP (n = 32) were penetrated by patient sperm whereas all (n = 32) ZP were penetrated by control sperm. Acrosome intact sperm removed from the ZP were used for intracytoplasmic sperm injection. All patients had > or = 50% normal fertilization and embryo development. Three pregnancies (one early aborted, two ongoing) were achieved after the transfer of fresh or frozen embryos. CONCLUSION: Acrosome-intact human sperm can produce a high fertilization rate and pregnancies after intracytoplasmic sperm injection in patients with disordered ZP-induced AR. The acrosome reaction is unlikely to be important for fertilization with intracytoplasmic sperm injection. SN - 0015-0282 UR - https://www.unboundmedicine.com/medline/citation/7789545/Fertilization_and_pregnancy_with_acrosome_intact_sperm_by_intracytoplasmic_sperm_injection_in_patients_with_disordered_zona_pellucida_induced_acrosome_reaction_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0015-0282(16)57666-3 DB - PRIME DP - Unbound Medicine ER -