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Mutations affecting donor preference during mating type interconversion in Saccharomyces cerevisiae.
Genetics. 1995 Apr; 139(4):1495-510.G

Abstract

Homothallic strains of Saccharomyces cerevisiae can convert mating type from a to alpha or alpha to a as often as every generation, by replacing genetic information specifying one mating type at the expressor locus, MAT, with information specifying the opposite mating type. The cryptic mating type information that is copied and inserted at MAT is contained in either of two loci, HML or HMR. The particular locus selected as donor during mating type interconversion is regulated by the allele expressed at MAT. MATa cells usually select HML, and MAT alpha cells usually select HMR, a process referred to as donor preference. To identify factors required for donor preference, we isolated and characterized a number of mutants that frequently selected the nonpreferred donor locus during mating type interconversion. Many of these mutants were found to harbor chromosome rearrangements or mutations at MAT or HML that interfered with the switching process. However, one mutant carried a recessive allele of CHL1, a gene previously shown to be required for efficient chromosome segregation during mitosis. Homothallic strains of yeast containing a null allele of CHL1 exhibited almost random selection of the donor locus in a MATa background but were normal in their ability to select HMR in a MAT alpha background. Our results indicate that Chl1p participates in the process of donor selection and are consistent with a model in which Chl1p helps establish an intrinsic bias in donor preference.

Authors+Show Affiliations

Department of Molecular Biology, Princeton University, New Jersey 08544-1014, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7789755

Citation

Weiler, K S., et al. "Mutations Affecting Donor Preference During Mating Type Interconversion in Saccharomyces Cerevisiae." Genetics, vol. 139, no. 4, 1995, pp. 1495-510.
Weiler KS, Szeto L, Broach JR. Mutations affecting donor preference during mating type interconversion in Saccharomyces cerevisiae. Genetics. 1995;139(4):1495-510.
Weiler, K. S., Szeto, L., & Broach, J. R. (1995). Mutations affecting donor preference during mating type interconversion in Saccharomyces cerevisiae. Genetics, 139(4), 1495-510.
Weiler KS, Szeto L, Broach JR. Mutations Affecting Donor Preference During Mating Type Interconversion in Saccharomyces Cerevisiae. Genetics. 1995;139(4):1495-510. PubMed PMID: 7789755.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mutations affecting donor preference during mating type interconversion in Saccharomyces cerevisiae. AU - Weiler,K S, AU - Szeto,L, AU - Broach,J R, PY - 1995/4/1/pubmed PY - 1995/4/1/medline PY - 1995/4/1/entrez SP - 1495 EP - 510 JF - Genetics JO - Genetics VL - 139 IS - 4 N2 - Homothallic strains of Saccharomyces cerevisiae can convert mating type from a to alpha or alpha to a as often as every generation, by replacing genetic information specifying one mating type at the expressor locus, MAT, with information specifying the opposite mating type. The cryptic mating type information that is copied and inserted at MAT is contained in either of two loci, HML or HMR. The particular locus selected as donor during mating type interconversion is regulated by the allele expressed at MAT. MATa cells usually select HML, and MAT alpha cells usually select HMR, a process referred to as donor preference. To identify factors required for donor preference, we isolated and characterized a number of mutants that frequently selected the nonpreferred donor locus during mating type interconversion. Many of these mutants were found to harbor chromosome rearrangements or mutations at MAT or HML that interfered with the switching process. However, one mutant carried a recessive allele of CHL1, a gene previously shown to be required for efficient chromosome segregation during mitosis. Homothallic strains of yeast containing a null allele of CHL1 exhibited almost random selection of the donor locus in a MATa background but were normal in their ability to select HMR in a MAT alpha background. Our results indicate that Chl1p participates in the process of donor selection and are consistent with a model in which Chl1p helps establish an intrinsic bias in donor preference. SN - 0016-6731 UR - https://www.unboundmedicine.com/medline/citation/7789755/Mutations_affecting_donor_preference_during_mating_type_interconversion_in_Saccharomyces_cerevisiae_ L2 - https://academic.oup.com/genetics/article-lookup/doi/10.1093/genetics/139.4.1495 DB - PRIME DP - Unbound Medicine ER -