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Characterization of intestinal invasion by Salmonella typhimurium and Salmonella dublin and effect of a mutation in the invH gene.
Infect Immun. 1995 Jul; 63(7):2743-54.II

Abstract

The relative levels of invasiveness of two bovine isolates each of Salmonella typhimurium and Salmonella dublin and of invH mutants of S. typhimurium were determined in MDCK and Int 407 cultured-cell assays and in bovine ileal loops. S. dublin was found to be significantly less invasive in cultured cells than S. typhimurium, but this difference was not observed in bovine intestines. The invH mutants exhibited a significant reduction in invasion in both cultured cells and bovine intestines. The invasive phenotypes of the strains were confirmed by fluorescent microscopy and scanning and transmission electron microscopy. The wild-type strains were observed in the laminae propriae of the intestinal villi, while in contrast the invH mutants were generally associated with the enterocyte layer. The degree of damage in the bovine ileum was related to the magnitude of the invasion. There was no difference in the amount of S. typhimurium or S. dublin recovered from the bovine ileum either with or without Peyer's patches 3 h after inoculation of the loop.

Authors+Show Affiliations

Institute for Animal Health, Compton, Newbury, Berkshire, United Kingdom.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7790093

Citation

Watson, P R., et al. "Characterization of Intestinal Invasion By Salmonella Typhimurium and Salmonella Dublin and Effect of a Mutation in the invH Gene." Infection and Immunity, vol. 63, no. 7, 1995, pp. 2743-54.
Watson PR, Paulin SM, Bland AP, et al. Characterization of intestinal invasion by Salmonella typhimurium and Salmonella dublin and effect of a mutation in the invH gene. Infect Immun. 1995;63(7):2743-54.
Watson, P. R., Paulin, S. M., Bland, A. P., Jones, P. W., & Wallis, T. S. (1995). Characterization of intestinal invasion by Salmonella typhimurium and Salmonella dublin and effect of a mutation in the invH gene. Infection and Immunity, 63(7), 2743-54.
Watson PR, et al. Characterization of Intestinal Invasion By Salmonella Typhimurium and Salmonella Dublin and Effect of a Mutation in the invH Gene. Infect Immun. 1995;63(7):2743-54. PubMed PMID: 7790093.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of intestinal invasion by Salmonella typhimurium and Salmonella dublin and effect of a mutation in the invH gene. AU - Watson,P R, AU - Paulin,S M, AU - Bland,A P, AU - Jones,P W, AU - Wallis,T S, PY - 1995/7/1/pubmed PY - 1995/7/1/medline PY - 1995/7/1/entrez SP - 2743 EP - 54 JF - Infection and immunity JO - Infect Immun VL - 63 IS - 7 N2 - The relative levels of invasiveness of two bovine isolates each of Salmonella typhimurium and Salmonella dublin and of invH mutants of S. typhimurium were determined in MDCK and Int 407 cultured-cell assays and in bovine ileal loops. S. dublin was found to be significantly less invasive in cultured cells than S. typhimurium, but this difference was not observed in bovine intestines. The invH mutants exhibited a significant reduction in invasion in both cultured cells and bovine intestines. The invasive phenotypes of the strains were confirmed by fluorescent microscopy and scanning and transmission electron microscopy. The wild-type strains were observed in the laminae propriae of the intestinal villi, while in contrast the invH mutants were generally associated with the enterocyte layer. The degree of damage in the bovine ileum was related to the magnitude of the invasion. There was no difference in the amount of S. typhimurium or S. dublin recovered from the bovine ileum either with or without Peyer's patches 3 h after inoculation of the loop. SN - 0019-9567 UR - https://www.unboundmedicine.com/medline/citation/7790093/Characterization_of_intestinal_invasion_by_Salmonella_typhimurium_and_Salmonella_dublin_and_effect_of_a_mutation_in_the_invH_gene_ L2 - https://journals.asm.org/doi/10.1128/iai.63.7.2743-2754.1995?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -