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Detection of Chlamydia trachomatis in urine specimens from women by ligase chain reaction.
J Clin Microbiol. 1995 Apr; 33(4):898-900.JC

Abstract

The performance of a plasmid-based ligase chain reaction (LCR) with urine specimens was compared with those of cell culture of cervical swabs and enzyme immunoassay with urine specimens for the detection of Chlamydia trachomatis infection in women who had attended a family planning clinic. The prevalence of chlamydial infection determined by LCR was 3.1%. Discrepant results among the three assays were resolved by testing urine by a second LCR assay based on the C. trachomatis chromosomal gene encoding the major outer membrane protein. Sensitivity, specificity, and positive and negative predictive values for the cell cultures were 56.3, 100, 100, and 98.4%, respectively, whereas those for the enzyme immunoassay were 18.8, 100, 100, and 97.1%, respectively, and those for LCR were 87.5, 100, 100, and 99.5%, respectively. LCR thus provides a highly sensitive and specific noninvasive screening method for detecting genital chlamydial infections in women.

Authors+Show Affiliations

Institute of Clinical Bacteriology, University of Uppsala, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

7790456

Citation

Bassiri, M, et al. "Detection of Chlamydia Trachomatis in Urine Specimens From Women By Ligase Chain Reaction." Journal of Clinical Microbiology, vol. 33, no. 4, 1995, pp. 898-900.
Bassiri M, Hu HY, Domeika MA, et al. Detection of Chlamydia trachomatis in urine specimens from women by ligase chain reaction. J Clin Microbiol. 1995;33(4):898-900.
Bassiri, M., Hu, H. Y., Domeika, M. A., Burczak, J., Svensson, L. O., Lee, H. H., & Mårdh, P. A. (1995). Detection of Chlamydia trachomatis in urine specimens from women by ligase chain reaction. Journal of Clinical Microbiology, 33(4), 898-900.
Bassiri M, et al. Detection of Chlamydia Trachomatis in Urine Specimens From Women By Ligase Chain Reaction. J Clin Microbiol. 1995;33(4):898-900. PubMed PMID: 7790456.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of Chlamydia trachomatis in urine specimens from women by ligase chain reaction. AU - Bassiri,M, AU - Hu,H Y, AU - Domeika,M A, AU - Burczak,J, AU - Svensson,L O, AU - Lee,H H, AU - Mårdh,P A, PY - 1995/4/1/pubmed PY - 1995/4/1/medline PY - 1995/4/1/entrez SP - 898 EP - 900 JF - Journal of clinical microbiology JO - J. Clin. Microbiol. VL - 33 IS - 4 N2 - The performance of a plasmid-based ligase chain reaction (LCR) with urine specimens was compared with those of cell culture of cervical swabs and enzyme immunoassay with urine specimens for the detection of Chlamydia trachomatis infection in women who had attended a family planning clinic. The prevalence of chlamydial infection determined by LCR was 3.1%. Discrepant results among the three assays were resolved by testing urine by a second LCR assay based on the C. trachomatis chromosomal gene encoding the major outer membrane protein. Sensitivity, specificity, and positive and negative predictive values for the cell cultures were 56.3, 100, 100, and 98.4%, respectively, whereas those for the enzyme immunoassay were 18.8, 100, 100, and 97.1%, respectively, and those for LCR were 87.5, 100, 100, and 99.5%, respectively. LCR thus provides a highly sensitive and specific noninvasive screening method for detecting genital chlamydial infections in women. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/7790456/Detection_of_Chlamydia_trachomatis_in_urine_specimens_from_women_by_ligase_chain_reaction_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=7790456 DB - PRIME DP - Unbound Medicine ER -