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DNA structure-dependent requirements for yeast RAD genes in gene conversion.
Nature. 1995 Jan 05; 373(6509):84-6.Nat

Abstract

In Saccharomyces cerevisiae, HO endonuclease-induced mating-type (MAT) switching is a specialized mitotic recombination event in which MAT sequences are replaced by those copied from a distant, unexpressed donor (HML or HMR). The donors have a chromatin structure inaccessible for both transcription and HO cleavage. Here we use physical monitoring of DNA to show that MAT switching is completely blocked at an early step in recombination in strains deleted for the DNA repair genes RAD51, RAD52, RAD54, RAD55 or RAD57. We find, however, that only RAD52 is required when the donor sequence is simultaneously not silenced and located on a plasmid. RAD51, RAD54, RAD55 and RAD57 are still required when the same transcribed donor is on the chromosome. We conclude that recombination in vivo occurs between DNA molecules in chromatin, whose structure significantly influences the outcome. RAD51, RAD54, RAD55 and RAD57 are all required to facilitate strand invasion into otherwise inaccessible donor sequences.

Authors+Show Affiliations

Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts 02254-9110.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7800045

Citation

Sugawara, N, et al. "DNA Structure-dependent Requirements for Yeast RAD Genes in Gene Conversion." Nature, vol. 373, no. 6509, 1995, pp. 84-6.
Sugawara N, Ivanov EL, Fishman-Lobell J, et al. DNA structure-dependent requirements for yeast RAD genes in gene conversion. Nature. 1995;373(6509):84-6.
Sugawara, N., Ivanov, E. L., Fishman-Lobell, J., Ray, B. L., Wu, X., & Haber, J. E. (1995). DNA structure-dependent requirements for yeast RAD genes in gene conversion. Nature, 373(6509), 84-6.
Sugawara N, et al. DNA Structure-dependent Requirements for Yeast RAD Genes in Gene Conversion. Nature. 1995 Jan 5;373(6509):84-6. PubMed PMID: 7800045.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DNA structure-dependent requirements for yeast RAD genes in gene conversion. AU - Sugawara,N, AU - Ivanov,E L, AU - Fishman-Lobell,J, AU - Ray,B L, AU - Wu,X, AU - Haber,J E, PY - 1995/1/5/pubmed PY - 1995/1/5/medline PY - 1995/1/5/entrez SP - 84 EP - 6 JF - Nature JO - Nature VL - 373 IS - 6509 N2 - In Saccharomyces cerevisiae, HO endonuclease-induced mating-type (MAT) switching is a specialized mitotic recombination event in which MAT sequences are replaced by those copied from a distant, unexpressed donor (HML or HMR). The donors have a chromatin structure inaccessible for both transcription and HO cleavage. Here we use physical monitoring of DNA to show that MAT switching is completely blocked at an early step in recombination in strains deleted for the DNA repair genes RAD51, RAD52, RAD54, RAD55 or RAD57. We find, however, that only RAD52 is required when the donor sequence is simultaneously not silenced and located on a plasmid. RAD51, RAD54, RAD55 and RAD57 are still required when the same transcribed donor is on the chromosome. We conclude that recombination in vivo occurs between DNA molecules in chromatin, whose structure significantly influences the outcome. RAD51, RAD54, RAD55 and RAD57 are all required to facilitate strand invasion into otherwise inaccessible donor sequences. SN - 0028-0836 UR - https://www.unboundmedicine.com/medline/citation/7800045/DNA_structure_dependent_requirements_for_yeast_RAD_genes_in_gene_conversion_ L2 - https://doi.org/10.1038/373084a0 DB - PRIME DP - Unbound Medicine ER -