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Platelet-activating factor (PAF) positively auto-regulates the expression of human PAF receptor transcript 1 (leukocyte-type) through NF-kappa B.
Biochem Biophys Res Commun. 1994 Dec 15; 205(2):1137-42.BB

Abstract

The human platelet-activating factor receptor (PAFR) gene is transcribed by two distinct promoters (promoter 1 and promoter 2) to generate two transcripts (designated as PAFR transcript 1 and PAFR transcript 2), though their open reading frames are identical. By primer extension analysis to discriminate two transcripts, we found that the levels of PAFR transcript 1 (leukocyte-type), but not PAFR transcript 2 (tissue-type), are upregulated by PAF as well as by 12-O-tetradecanoylphorbol-13-acetate (TPA) in the human stomach cancer cell line (JR-St cells) which expresses both functional PAFR transcript 1 and PAFR transcript 2 endogenously. Functional analysis of the promoter 1 with a transient expression assay using chloramphenicol acetyltransferase (CAT) gene as a reporter showed that both PAF and TPA activated the promoter 1 but not the deleted promoter lacking the three consensus binding sites for NF-kappa B located from -571 bp to -459 bp. These findings suggest a molecular mechanism of positive regulation of PAFR gene expression by PAF through NF-kappa B, possibly by a phosphorylation reaction involving protein kinase C by PAF.

Authors+Show Affiliations

Department of Biochemistry, Faculty of Medicine, University of Tokyo, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7802642

Citation

Mutoh, H, et al. "Platelet-activating Factor (PAF) Positively Auto-regulates the Expression of Human PAF Receptor Transcript 1 (leukocyte-type) Through NF-kappa B." Biochemical and Biophysical Research Communications, vol. 205, no. 2, 1994, pp. 1137-42.
Mutoh H, Ishii S, Izumi T, et al. Platelet-activating factor (PAF) positively auto-regulates the expression of human PAF receptor transcript 1 (leukocyte-type) through NF-kappa B. Biochem Biophys Res Commun. 1994;205(2):1137-42.
Mutoh, H., Ishii, S., Izumi, T., Kato, S., & Shimizu, T. (1994). Platelet-activating factor (PAF) positively auto-regulates the expression of human PAF receptor transcript 1 (leukocyte-type) through NF-kappa B. Biochemical and Biophysical Research Communications, 205(2), 1137-42.
Mutoh H, et al. Platelet-activating Factor (PAF) Positively Auto-regulates the Expression of Human PAF Receptor Transcript 1 (leukocyte-type) Through NF-kappa B. Biochem Biophys Res Commun. 1994 Dec 15;205(2):1137-42. PubMed PMID: 7802642.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Platelet-activating factor (PAF) positively auto-regulates the expression of human PAF receptor transcript 1 (leukocyte-type) through NF-kappa B. AU - Mutoh,H, AU - Ishii,S, AU - Izumi,T, AU - Kato,S, AU - Shimizu,T, PY - 1994/12/15/pubmed PY - 1994/12/15/medline PY - 1994/12/15/entrez SP - 1137 EP - 42 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 205 IS - 2 N2 - The human platelet-activating factor receptor (PAFR) gene is transcribed by two distinct promoters (promoter 1 and promoter 2) to generate two transcripts (designated as PAFR transcript 1 and PAFR transcript 2), though their open reading frames are identical. By primer extension analysis to discriminate two transcripts, we found that the levels of PAFR transcript 1 (leukocyte-type), but not PAFR transcript 2 (tissue-type), are upregulated by PAF as well as by 12-O-tetradecanoylphorbol-13-acetate (TPA) in the human stomach cancer cell line (JR-St cells) which expresses both functional PAFR transcript 1 and PAFR transcript 2 endogenously. Functional analysis of the promoter 1 with a transient expression assay using chloramphenicol acetyltransferase (CAT) gene as a reporter showed that both PAF and TPA activated the promoter 1 but not the deleted promoter lacking the three consensus binding sites for NF-kappa B located from -571 bp to -459 bp. These findings suggest a molecular mechanism of positive regulation of PAFR gene expression by PAF through NF-kappa B, possibly by a phosphorylation reaction involving protein kinase C by PAF. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/7802642/Platelet_activating_factor__PAF__positively_auto_regulates_the_expression_of_human_PAF_receptor_transcript_1__leukocyte_type__through_NF_kappa_B_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(84)72784-7 DB - PRIME DP - Unbound Medicine ER -