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Long-term effects of deprivation of cell support in the distal stump on peripheral nerve regeneration.
J Neurosci Res. 1994 Sep 01; 39(1):23-30.JN

Abstract

The distal stump of an injured peripheral nerve supports regenerating axons by offering a favourable growth substratum and several cell-produced growth factors. Deprivation of cellular factors alone has been shown not to prevent fairly rapid axonal elongation after nerve injury if the growth substratum was preserved. The present study examined possible long-term untoward effects of cell support deprivation during an early phase of nerve regeneration. Rat sciatic nerve was crushed and a 25 mm long distal nerve segment was made acellular by freezing-thawing, while the integrity of the growth substratum for the regenerating axons was preserved. Toe-spreading reflex and skin sensitivity to pinch in the foot were monitored to follow recovery of motor and sensory function, respectively. The number of myelinated axons was determined in the sciatic nerve proximally to the lesion site, and distally in the predominantly sensory sural nerve as well as in the mixed motor nerve to the soleus muscle. Except for a short delay in the onset of recovery, explainable by the reduced elongation rate of axons growing through the acellular nerve segment, we found no deleterious effect of cell support deprivation on sensory or motor function recovery after nerve crush. Most of regenerating sensory neurons did not critically depend on the distal stump cell support. However, a 15% and 25% loss of myelinated axons both proximally to the lesion and distally in the sensory sural nerve, respectively, indicated that a corresponding minor loss of injured sensory neurons occurred when they were deprived of such cell support even if provided with a favourable growth substratum for successful regeneration.(ABSTRACT TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Institute of Pathophysiology, School of Medicine, Ljubljana, Slovenia.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7807589

Citation

Bajrović, F, et al. "Long-term Effects of Deprivation of Cell Support in the Distal Stump On Peripheral Nerve Regeneration." Journal of Neuroscience Research, vol. 39, no. 1, 1994, pp. 23-30.
Bajrović F, Bresjanac M, Sketelj J. Long-term effects of deprivation of cell support in the distal stump on peripheral nerve regeneration. J Neurosci Res. 1994;39(1):23-30.
Bajrović, F., Bresjanac, M., & Sketelj, J. (1994). Long-term effects of deprivation of cell support in the distal stump on peripheral nerve regeneration. Journal of Neuroscience Research, 39(1), 23-30.
Bajrović F, Bresjanac M, Sketelj J. Long-term Effects of Deprivation of Cell Support in the Distal Stump On Peripheral Nerve Regeneration. J Neurosci Res. 1994 Sep 1;39(1):23-30. PubMed PMID: 7807589.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Long-term effects of deprivation of cell support in the distal stump on peripheral nerve regeneration. AU - Bajrović,F, AU - Bresjanac,M, AU - Sketelj,J, PY - 1994/9/1/pubmed PY - 1994/9/1/medline PY - 1994/9/1/entrez SP - 23 EP - 30 JF - Journal of neuroscience research JO - J Neurosci Res VL - 39 IS - 1 N2 - The distal stump of an injured peripheral nerve supports regenerating axons by offering a favourable growth substratum and several cell-produced growth factors. Deprivation of cellular factors alone has been shown not to prevent fairly rapid axonal elongation after nerve injury if the growth substratum was preserved. The present study examined possible long-term untoward effects of cell support deprivation during an early phase of nerve regeneration. Rat sciatic nerve was crushed and a 25 mm long distal nerve segment was made acellular by freezing-thawing, while the integrity of the growth substratum for the regenerating axons was preserved. Toe-spreading reflex and skin sensitivity to pinch in the foot were monitored to follow recovery of motor and sensory function, respectively. The number of myelinated axons was determined in the sciatic nerve proximally to the lesion site, and distally in the predominantly sensory sural nerve as well as in the mixed motor nerve to the soleus muscle. Except for a short delay in the onset of recovery, explainable by the reduced elongation rate of axons growing through the acellular nerve segment, we found no deleterious effect of cell support deprivation on sensory or motor function recovery after nerve crush. Most of regenerating sensory neurons did not critically depend on the distal stump cell support. However, a 15% and 25% loss of myelinated axons both proximally to the lesion and distally in the sensory sural nerve, respectively, indicated that a corresponding minor loss of injured sensory neurons occurred when they were deprived of such cell support even if provided with a favourable growth substratum for successful regeneration.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0360-4012 UR - https://www.unboundmedicine.com/medline/citation/7807589/Long_term_effects_of_deprivation_of_cell_support_in_the_distal_stump_on_peripheral_nerve_regeneration_ DB - PRIME DP - Unbound Medicine ER -