Tags

Type your tag names separated by a space and hit enter

Rat serum inhibits progression of alveolar epithelial cells toward the type I cell phenotype in vitro.
Am J Respir Cell Mol Biol. 1995 Jan; 12(1):50-5.AJ

Abstract

Serum contains a number of polypeptide growth factors, hormones, and soluble matrix components and may influence the state of differentiation of epithelial cells in general and of alveolar epithelial cells (AEC) in particular. To evaluate the influence of sera on the transition from the type II toward the type I cell phenotype, we compared the effects of newborn bovine serum (NBS) and rat serum (RS) on morphologic changes and expression of a type I cell-specific epitope in AEC monolayers with time in primary culture. Rat type II AEC were harvested and cultured in defined serum-free medium (MDSF), MDSF + RS (5%), or MDSF + NBS (10%). Monolayer integrity was monitored by measuring transepithelial resistance (approximately 2,000 omega.cm2) and short-circuit current (approximately 4 microA/cm2). Binding of the type I cell-specific monoclonal antibody VIIIB2 was assessed between day 1 and day 11 by cell-based enzyme-linked immunosorbent assay (ELISA) and by immunoelectron microscopy (IEM). By ELISA, in MDSF and MDSF + NBS, VIIIB2 binding increased markedly after day 2, rising approximately 4-fold by day 8 (compared with day 1). In dramatic contrast, there was essentially no increase in VIIIB2 binding through day 11 in MDSF + RS. Results from IEM for apical surface binding of VIIIB2 were similar to those obtained by ELISA. Some morphologic differences were also noted, with cells in MDSF + RS being somewhat less spread at later times than those in MDSF or MDSF + NBS. These data indicate that the rate of rat type II AEC differentiation toward the type I cell phenotype is significantly modulated by soluble factor(s) present in rat serum.

Authors+Show Affiliations

Will Rogers Institute Pulmonary Research Center, Division of Pulmonary and Critical Care Medicine, University of Southern California, Los Angeles 90033.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7811470

Citation

Borok, Z, et al. "Rat Serum Inhibits Progression of Alveolar Epithelial Cells Toward the Type I Cell Phenotype in Vitro." American Journal of Respiratory Cell and Molecular Biology, vol. 12, no. 1, 1995, pp. 50-5.
Borok Z, Hami A, Danto SI, et al. Rat serum inhibits progression of alveolar epithelial cells toward the type I cell phenotype in vitro. Am J Respir Cell Mol Biol. 1995;12(1):50-5.
Borok, Z., Hami, A., Danto, S. I., Zabski, S. M., & Crandall, E. D. (1995). Rat serum inhibits progression of alveolar epithelial cells toward the type I cell phenotype in vitro. American Journal of Respiratory Cell and Molecular Biology, 12(1), 50-5.
Borok Z, et al. Rat Serum Inhibits Progression of Alveolar Epithelial Cells Toward the Type I Cell Phenotype in Vitro. Am J Respir Cell Mol Biol. 1995;12(1):50-5. PubMed PMID: 7811470.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rat serum inhibits progression of alveolar epithelial cells toward the type I cell phenotype in vitro. AU - Borok,Z, AU - Hami,A, AU - Danto,S I, AU - Zabski,S M, AU - Crandall,E D, PY - 1995/1/1/pubmed PY - 1995/1/1/medline PY - 1995/1/1/entrez SP - 50 EP - 5 JF - American journal of respiratory cell and molecular biology JO - Am. J. Respir. Cell Mol. Biol. VL - 12 IS - 1 N2 - Serum contains a number of polypeptide growth factors, hormones, and soluble matrix components and may influence the state of differentiation of epithelial cells in general and of alveolar epithelial cells (AEC) in particular. To evaluate the influence of sera on the transition from the type II toward the type I cell phenotype, we compared the effects of newborn bovine serum (NBS) and rat serum (RS) on morphologic changes and expression of a type I cell-specific epitope in AEC monolayers with time in primary culture. Rat type II AEC were harvested and cultured in defined serum-free medium (MDSF), MDSF + RS (5%), or MDSF + NBS (10%). Monolayer integrity was monitored by measuring transepithelial resistance (approximately 2,000 omega.cm2) and short-circuit current (approximately 4 microA/cm2). Binding of the type I cell-specific monoclonal antibody VIIIB2 was assessed between day 1 and day 11 by cell-based enzyme-linked immunosorbent assay (ELISA) and by immunoelectron microscopy (IEM). By ELISA, in MDSF and MDSF + NBS, VIIIB2 binding increased markedly after day 2, rising approximately 4-fold by day 8 (compared with day 1). In dramatic contrast, there was essentially no increase in VIIIB2 binding through day 11 in MDSF + RS. Results from IEM for apical surface binding of VIIIB2 were similar to those obtained by ELISA. Some morphologic differences were also noted, with cells in MDSF + RS being somewhat less spread at later times than those in MDSF or MDSF + NBS. These data indicate that the rate of rat type II AEC differentiation toward the type I cell phenotype is significantly modulated by soluble factor(s) present in rat serum. SN - 1044-1549 UR - https://www.unboundmedicine.com/medline/citation/7811470/Rat_serum_inhibits_progression_of_alveolar_epithelial_cells_toward_the_type_I_cell_phenotype_in_vitro_ L2 - http://www.atsjournals.org/doi/full/10.1165/ajrcmb.12.1.7811470?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -