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Induction of MHC class II molecules HLA-DR, -DP and -DQ and ICAM 1 in human podocytes by gamma-interferon.
Exp Nephrol. 1994 Sep-Oct; 2(5):306-12.EN

Abstract

MHC class II-encoded molecules HLA-DR, -DP and -DQ play a pivotal role in the human immune response. Their constitutive expression is restricted to a number of immunocompetent cells referred to as antigen-presenting cells. However, gamma-interferon (gamma-IFN) has been shown to induce MHC class II molecule expression in several epithelia. Using flow cytometric analysis, we show here that normal and SV40-transformed human podocytes in culture constitutively expressed gamma-IFN receptors. We also show that MHC class I molecules are constitutively expressed in these cells and that HLA-DR, -DP and -DQ expression, which is not found in unstimulated cells, can be induced by gamma-IFN stimulation. This induction was a time-dependent event, a lag phase of 24-48 h being necessary for MHC class II molecules to become detectable at the cell surface by flow cytometric analysis. Induction of MHC class II molecules in human podocytes also showed a concentration dependence, a plateau being reached at a concentration of 500 IU of gamma-IFN/ml of culture medium. This effect was blunted by coincubation of the cells with an antihuman gamma-IFN receptor monoclonal antibody. HLA-DR expression was associated with specific mRNA accumulation, as detected by Northern blot analysis. By indirect immunofluorescence, the intercellular adhesion molecule 1 was also induced by gamma-IFN stimulation. Induction of DR, DP and DQ in human podocytes may be involved in the pathogenesis of immune glomerulonephritis in man.

Authors+Show Affiliations

Service de Néphrologie A and INSERM, U-64, Hôpital Tenon, Paris, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7812737

Citation

Baudeau, C, et al. "Induction of MHC Class II Molecules HLA-DR, -DP and -DQ and ICAM 1 in Human Podocytes By Gamma-interferon." Experimental Nephrology, vol. 2, no. 5, 1994, pp. 306-12.
Baudeau C, Delarue F, Hé CJ, et al. Induction of MHC class II molecules HLA-DR, -DP and -DQ and ICAM 1 in human podocytes by gamma-interferon. Exp Nephrol. 1994;2(5):306-12.
Baudeau, C., Delarue, F., Hé, C. J., Nguyen, G., Adida, C., Peraldi, M. N., Sraer, J. D., & Rondeau, E. (1994). Induction of MHC class II molecules HLA-DR, -DP and -DQ and ICAM 1 in human podocytes by gamma-interferon. Experimental Nephrology, 2(5), 306-12.
Baudeau C, et al. Induction of MHC Class II Molecules HLA-DR, -DP and -DQ and ICAM 1 in Human Podocytes By Gamma-interferon. Exp Nephrol. 1994 Sep-Oct;2(5):306-12. PubMed PMID: 7812737.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Induction of MHC class II molecules HLA-DR, -DP and -DQ and ICAM 1 in human podocytes by gamma-interferon. AU - Baudeau,C, AU - Delarue,F, AU - Hé,C J, AU - Nguyen,G, AU - Adida,C, AU - Peraldi,M N, AU - Sraer,J D, AU - Rondeau,E, PY - 1994/9/1/pubmed PY - 1994/9/1/medline PY - 1994/9/1/entrez SP - 306 EP - 12 JF - Experimental nephrology JO - Exp Nephrol VL - 2 IS - 5 N2 - MHC class II-encoded molecules HLA-DR, -DP and -DQ play a pivotal role in the human immune response. Their constitutive expression is restricted to a number of immunocompetent cells referred to as antigen-presenting cells. However, gamma-interferon (gamma-IFN) has been shown to induce MHC class II molecule expression in several epithelia. Using flow cytometric analysis, we show here that normal and SV40-transformed human podocytes in culture constitutively expressed gamma-IFN receptors. We also show that MHC class I molecules are constitutively expressed in these cells and that HLA-DR, -DP and -DQ expression, which is not found in unstimulated cells, can be induced by gamma-IFN stimulation. This induction was a time-dependent event, a lag phase of 24-48 h being necessary for MHC class II molecules to become detectable at the cell surface by flow cytometric analysis. Induction of MHC class II molecules in human podocytes also showed a concentration dependence, a plateau being reached at a concentration of 500 IU of gamma-IFN/ml of culture medium. This effect was blunted by coincubation of the cells with an antihuman gamma-IFN receptor monoclonal antibody. HLA-DR expression was associated with specific mRNA accumulation, as detected by Northern blot analysis. By indirect immunofluorescence, the intercellular adhesion molecule 1 was also induced by gamma-IFN stimulation. Induction of DR, DP and DQ in human podocytes may be involved in the pathogenesis of immune glomerulonephritis in man. SN - 1018-7782 UR - https://www.unboundmedicine.com/medline/citation/7812737/Induction_of_MHC_class_II_molecules_HLA_DR__DP_and__DQ_and_ICAM_1_in_human_podocytes_by_gamma_interferon_ L2 - https://antibodies.cancer.gov/detail/CPTC-HLA-DPB1-2 DB - PRIME DP - Unbound Medicine ER -