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HTLV-I and HTLV-II infections among HIV-1 seropositive patients in Sao Paulo, Brazil.
Eur J Epidemiol. 1994 Apr; 10(2):165-71.EJ

Abstract

To estimate the presence of, and the risk factors for HTLV-I and HTLV-II infections among HIV-1 infected subjects in Sao Paulo, Brazil, a serosurvey was performed in 471 HIV-1 infected patients, including 216 intravenous drug addicts (IVDA), 229 homosexual/bisexual men, and 26 with other risk factors. Serum samples were screened for HTLV seroreactivity by ELISA; reactive samples were analyzed by Western Blot (WB), using whole HTLV-I lysate as antigen. To confirm and discriminate HTLV-I and HTLV-II infections, sera presenting any bands on WB were further analyzed by a WB containing recombinant HTLV-I and HTLV-II proteins (WB 2.3), and by enzyme immunoassays using synthetic peptides specific for envelope proteins (Synth-EIA). In 22 cases, cell samples were available for polymerase chain reaction (PCR) studies. On WB, 114 sera were reactive and, of these, 37 and 25 were concordantly positive on both WB 2.3 and Synth-EIA procedures for HTLV-I and HTLV-II specific antibodies, respectively; 37 specimens were negative on both assays, and 15 gave discordant or indeterminate results. PCR findings confirmed concordant results obtained in the discriminatory serological assays. The prevalence rates of HTLV-I and HTLV-II infections were 15.3% and 11.1% in IVDA, and 0.9% and 0.4% in homosexual/bisexual men, respectively. No case of HTLV-I/HTLV-II co-infection was found.

Authors+Show Affiliations

de Araujo AC
Department of Immunology, Instituto Adolfo Lutz, Sao Paulo, Brazil.
Casseb JS
No affiliation info available
Neitzert E
No affiliation info available
de Souza ML
No affiliation info available
Mammano F
No affiliation info available
Del Mistro A
No affiliation info available
De Rossi A
No affiliation info available
Chieco-Bianchi L
No affiliation info available

MeSH

AdolescentAdultAgedBisexualityBlotting, WesternBrazilComorbidityEnzyme-Linked Immunosorbent AssayFemaleHIV SeropositivityHIV-1HTLV-I InfectionsHTLV-II InfectionsHomosexuality, MaleHumansImmunoenzyme TechniquesMaleMiddle AgedPolymerase Chain ReactionPopulation SurveillancePrevalenceRisk FactorsSeroepidemiologic StudiesSubstance Abuse, IntravenousUrban Population

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7813694

Citation

de Araujo, A C., et al. "HTLV-I and HTLV-II Infections Among HIV-1 Seropositive Patients in Sao Paulo, Brazil." European Journal of Epidemiology, vol. 10, no. 2, 1994, pp. 165-71.
de Araujo AC, Casseb JS, Neitzert E, et al. HTLV-I and HTLV-II infections among HIV-1 seropositive patients in Sao Paulo, Brazil. Eur J Epidemiol. 1994;10(2):165-71.
de Araujo, A. C., Casseb, J. S., Neitzert, E., de Souza, M. L., Mammano, F., Del Mistro, A., De Rossi, A., & Chieco-Bianchi, L. (1994). HTLV-I and HTLV-II infections among HIV-1 seropositive patients in Sao Paulo, Brazil. European Journal of Epidemiology, 10(2), 165-71.
de Araujo AC, et al. HTLV-I and HTLV-II Infections Among HIV-1 Seropositive Patients in Sao Paulo, Brazil. Eur J Epidemiol. 1994;10(2):165-71. PubMed PMID: 7813694.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HTLV-I and HTLV-II infections among HIV-1 seropositive patients in Sao Paulo, Brazil. AU - de Araujo,A C, AU - Casseb,J S, AU - Neitzert,E, AU - de Souza,M L, AU - Mammano,F, AU - Del Mistro,A, AU - De Rossi,A, AU - Chieco-Bianchi,L, PY - 1994/4/1/pubmed PY - 1994/4/1/medline PY - 1994/4/1/entrez KW - Americas KW - Behavior KW - Brazil KW - Developing Countries KW - Diseases KW - Drug Usage KW - Examinations And Diagnoses KW - Hematologic Tests KW - Hiv Infections KW - Hiv Serodiagnosis KW - Iv Drug Users KW - Laboratory Examinations And Diagnoses KW - Laboratory Procedures KW - Latin America KW - Research Report KW - South America KW - Viral Diseases SP - 165 EP - 71 JF - European journal of epidemiology JO - Eur J Epidemiol VL - 10 IS - 2 N2 - To estimate the presence of, and the risk factors for HTLV-I and HTLV-II infections among HIV-1 infected subjects in Sao Paulo, Brazil, a serosurvey was performed in 471 HIV-1 infected patients, including 216 intravenous drug addicts (IVDA), 229 homosexual/bisexual men, and 26 with other risk factors. Serum samples were screened for HTLV seroreactivity by ELISA; reactive samples were analyzed by Western Blot (WB), using whole HTLV-I lysate as antigen. To confirm and discriminate HTLV-I and HTLV-II infections, sera presenting any bands on WB were further analyzed by a WB containing recombinant HTLV-I and HTLV-II proteins (WB 2.3), and by enzyme immunoassays using synthetic peptides specific for envelope proteins (Synth-EIA). In 22 cases, cell samples were available for polymerase chain reaction (PCR) studies. On WB, 114 sera were reactive and, of these, 37 and 25 were concordantly positive on both WB 2.3 and Synth-EIA procedures for HTLV-I and HTLV-II specific antibodies, respectively; 37 specimens were negative on both assays, and 15 gave discordant or indeterminate results. PCR findings confirmed concordant results obtained in the discriminatory serological assays. The prevalence rates of HTLV-I and HTLV-II infections were 15.3% and 11.1% in IVDA, and 0.9% and 0.4% in homosexual/bisexual men, respectively. No case of HTLV-I/HTLV-II co-infection was found. SN - 0393-2990 UR - https://www.unboundmedicine.com/medline/citation/7813694/HTLV_I_and_HTLV_II_infections_among_HIV_1_seropositive_patients_in_Sao_Paulo_Brazil_ L2 - http://www.diseaseinfosearch.org/result/9735 DB - PRIME DP - Unbound Medicine ER -