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Plasmid maintenance of derivatives of oriP of Epstein-Barr virus.
J Virol. 1995 Feb; 69(2):1280-3.JV

Abstract

oriP is the origin of plasmid replication of Epstein-Barr virus. Replication from oriP requires both the cis-acting elements (the family of repeats and the dyad symmetry element) and the viral origin-binding protein, EBNA-1. The ability of plasmids containing oriP to be maintained stably in EBNA-1-positive cells reflects the efficiency both of their replication and of their segregation each cell cycle. The efficiency of plasmid maintenance was determined for plasmids containing derivatives of oriP with one copy of the dyad symmetry element and two copies of the family of repeats by measuring the rate at which they were lost from cells in the absence of selection. These measurements demonstrated that plasmids with derivatives of oriP with two copies of the family of repeats in one orientation are maintained only slightly less efficiently than is wild-type oriP. To determine whether plasmid maintenance could be affected by reinitiation at the dyad symmetry element (T. A. Gahn and C. L. Schildkraut, Cell 58:527-535, 1989), plasmids containing derivatives of oriP with two copies of the dyad symmetry element and one copy of the family of repeats were compared with plasmids containing wild-type oriP in EBNA-1-positive cells. These measurements showed that plasmids containing a derivative of oriP with two copies of the dyad symmetry element are maintained as efficiently as is wild-type oriP and are not amplified relative to wild-type oriP. These observations indicate that the trans-acting factors that regulate DNA to replicate once per S phase are insensitive to multiple cis-acting regulatory sites within a replicon.

Authors+Show Affiliations

McArdle Laboratory for Cancer Research, University of Wisconsin Medical School, Madison 53706.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7815506

Citation

Kirchmaier, A L., and B Sugden. "Plasmid Maintenance of Derivatives of oriP of Epstein-Barr Virus." Journal of Virology, vol. 69, no. 2, 1995, pp. 1280-3.
Kirchmaier AL, Sugden B. Plasmid maintenance of derivatives of oriP of Epstein-Barr virus. J Virol. 1995;69(2):1280-3.
Kirchmaier, A. L., & Sugden, B. (1995). Plasmid maintenance of derivatives of oriP of Epstein-Barr virus. Journal of Virology, 69(2), 1280-3.
Kirchmaier AL, Sugden B. Plasmid Maintenance of Derivatives of oriP of Epstein-Barr Virus. J Virol. 1995;69(2):1280-3. PubMed PMID: 7815506.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Plasmid maintenance of derivatives of oriP of Epstein-Barr virus. AU - Kirchmaier,A L, AU - Sugden,B, PY - 1995/2/1/pubmed PY - 1995/2/1/medline PY - 1995/2/1/entrez SP - 1280 EP - 3 JF - Journal of virology JO - J Virol VL - 69 IS - 2 N2 - oriP is the origin of plasmid replication of Epstein-Barr virus. Replication from oriP requires both the cis-acting elements (the family of repeats and the dyad symmetry element) and the viral origin-binding protein, EBNA-1. The ability of plasmids containing oriP to be maintained stably in EBNA-1-positive cells reflects the efficiency both of their replication and of their segregation each cell cycle. The efficiency of plasmid maintenance was determined for plasmids containing derivatives of oriP with one copy of the dyad symmetry element and two copies of the family of repeats by measuring the rate at which they were lost from cells in the absence of selection. These measurements demonstrated that plasmids with derivatives of oriP with two copies of the family of repeats in one orientation are maintained only slightly less efficiently than is wild-type oriP. To determine whether plasmid maintenance could be affected by reinitiation at the dyad symmetry element (T. A. Gahn and C. L. Schildkraut, Cell 58:527-535, 1989), plasmids containing derivatives of oriP with two copies of the dyad symmetry element and one copy of the family of repeats were compared with plasmids containing wild-type oriP in EBNA-1-positive cells. These measurements showed that plasmids containing a derivative of oriP with two copies of the dyad symmetry element are maintained as efficiently as is wild-type oriP and are not amplified relative to wild-type oriP. These observations indicate that the trans-acting factors that regulate DNA to replicate once per S phase are insensitive to multiple cis-acting regulatory sites within a replicon. SN - 0022-538X UR - https://www.unboundmedicine.com/medline/citation/7815506/Plasmid_maintenance_of_derivatives_of_oriP_of_Epstein_Barr_virus_ L2 - https://journals.asm.org/doi/10.1128/JVI.69.2.1280-1283.1995?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -