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The interaction between the first and last intron nucleotides in the second step of pre-mRNA splicing is independent of other conserved intron nucleotides.
Nucleic Acids Res. 1994 Dec 11; 22(24):5190-5.NA

Abstract

Virtually all pre-mRNA introns begin with the sequence /GU and end with AG/ (where / indicates a border between an exon and an intron). We have previously shown that the G residues at the first and last positions of the yeast actin intron interact during the second step of splicing. In this work, we ask if other highly conserved intron nucleotides also take part in this /G-G/ interaction. Of special interest is the penultimate intron nucleotide (AG/), which is important for the second step of splicing and is in proximity to other conserved intron nucleotides. Therefore, we tested interactions of the penultimate intron nucleotide with the second intron nucleotide (/GU) and with the branch site nucleotide. We also tested two models that predict interactions between sets of three conserved intron nucleotides. In addition, we used random mutagenesis and genetic selection to search for interactions between nucleotides in the pre-mRNA. We find no evidence for other interactions between intron nucleotides besides the interaction between the first and last intron nucleotides.

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Authors+Show Affiliations

Ruis BL
Department of Biochemistry, University of Minnesota Medical School, Minneapolis 55455.
Kivens WJ
No affiliation info available
Siliciano PG
No affiliation info available

MeSH

Base SequenceConserved SequenceDNA, RecombinantIntronsMolecular Sequence DataMutationRNA PrecursorsRNA SplicingRNA, FungalRibonucleotidesYeasts

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

7816605

Citation

Ruis, B L., et al. "The Interaction Between the First and Last Intron Nucleotides in the Second Step of pre-mRNA Splicing Is Independent of Other Conserved Intron Nucleotides." Nucleic Acids Research, vol. 22, no. 24, 1994, pp. 5190-5.
Ruis BL, Kivens WJ, Siliciano PG. The interaction between the first and last intron nucleotides in the second step of pre-mRNA splicing is independent of other conserved intron nucleotides. Nucleic Acids Res. 1994;22(24):5190-5.
Ruis, B. L., Kivens, W. J., & Siliciano, P. G. (1994). The interaction between the first and last intron nucleotides in the second step of pre-mRNA splicing is independent of other conserved intron nucleotides. Nucleic Acids Research, 22(24), 5190-5.
Ruis BL, Kivens WJ, Siliciano PG. The Interaction Between the First and Last Intron Nucleotides in the Second Step of pre-mRNA Splicing Is Independent of Other Conserved Intron Nucleotides. Nucleic Acids Res. 1994 Dec 11;22(24):5190-5. PubMed PMID: 7816605.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The interaction between the first and last intron nucleotides in the second step of pre-mRNA splicing is independent of other conserved intron nucleotides. AU - Ruis,B L, AU - Kivens,W J, AU - Siliciano,P G, PY - 1994/12/11/pubmed PY - 1994/12/11/medline PY - 1994/12/11/entrez SP - 5190 EP - 5 JF - Nucleic acids research JO - Nucleic Acids Res VL - 22 IS - 24 N2 - Virtually all pre-mRNA introns begin with the sequence /GU and end with AG/ (where / indicates a border between an exon and an intron). We have previously shown that the G residues at the first and last positions of the yeast actin intron interact during the second step of splicing. In this work, we ask if other highly conserved intron nucleotides also take part in this /G-G/ interaction. Of special interest is the penultimate intron nucleotide (AG/), which is important for the second step of splicing and is in proximity to other conserved intron nucleotides. Therefore, we tested interactions of the penultimate intron nucleotide with the second intron nucleotide (/GU) and with the branch site nucleotide. We also tested two models that predict interactions between sets of three conserved intron nucleotides. In addition, we used random mutagenesis and genetic selection to search for interactions between nucleotides in the pre-mRNA. We find no evidence for other interactions between intron nucleotides besides the interaction between the first and last intron nucleotides. SN - 0305-1048 UR - https://www.unboundmedicine.com/medline/citation/7816605/The_interaction_between_the_first_and_last_intron_nucleotides_in_the_second_step_of_pre_mRNA_splicing_is_independent_of_other_conserved_intron_nucleotides_ L2 - https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/22.24.5190 DB - PRIME DP - Unbound Medicine ER -