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Simultaneous measurement of reticulocyte and red blood cell indices in healthy subjects and patients with microcytic and macrocytic anemia.

Abstract

Using the new Bayer H*3 hematology analyzer (Leverkusen, Germany), we have determined red blood cell and reticulocyte indices in 64 healthy subjects, in patients with microcytosis due to iron deficiency (58 patients) and heterozygous beta-thalassemia (40 patients), and in patients with macrocytosis (28 patients). We found in all cases that reticulocytes were larger than mature red cells by 24% to 35%, with a hemoglobin concentration 16% to 25% lower and a similar hemoglobin content. The correlation between red cell and reticulocyte indices was strikingly tight (r = .928 for volume, r = .929 for hemoglobin concentration, r = .972 for hemoglobin content) in all four groups, regardless of red blood cell size. The ratio of reticulocyte to red blood cell mean corpuscolar volume (MCV ratio) was constantly above 1. Inversion of the MCV ratio was observed only in four patients. It was always abrupt and transitory and was associated with erythropoietic changes leading to the production of red blood cells of a different volume (treatment of megaloblastic anemia, functional iron deficiency, bone marrow transplantation). In two cases of marrow transplantation, reticulocyte volume fell during the aplastic phase after conditioning chemotherapy and then rapidly increased up to values higher than before; this production of macroreticulocytes was the earliest sign of engraftment.

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  • Authors+Show Affiliations

    ,

    Research Center for the Development and Clinical Evaluation of Automated Methods in Hematology, Università Cattolica del Sacro Cuore, Rome, Italy.

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    Source

    Blood 85:3 1995 Feb 01 pg 818-23

    MeSH

    Anemia
    Anemia, Iron-Deficiency
    Anemia, Macrocytic
    Bone Marrow Transplantation
    Erythrocyte Count
    Erythrocytes
    Female
    Follow-Up Studies
    Humans
    Male
    Middle Aged
    Reference Values
    Regression Analysis
    Reticulocytes
    Transplantation, Homologous
    beta-Thalassemia

    Pub Type(s)

    Case Reports
    Clinical Trial
    Comparative Study
    Controlled Clinical Trial
    Journal Article

    Language

    eng

    PubMed ID

    7833482

    Citation

    d'Onofrio, G, et al. "Simultaneous Measurement of Reticulocyte and Red Blood Cell Indices in Healthy Subjects and Patients With Microcytic and Macrocytic Anemia." Blood, vol. 85, no. 3, 1995, pp. 818-23.
    d'Onofrio G, Chirillo R, Zini G, et al. Simultaneous measurement of reticulocyte and red blood cell indices in healthy subjects and patients with microcytic and macrocytic anemia. Blood. 1995;85(3):818-23.
    d'Onofrio, G., Chirillo, R., Zini, G., Caenaro, G., Tommasi, M., & Micciulli, G. (1995). Simultaneous measurement of reticulocyte and red blood cell indices in healthy subjects and patients with microcytic and macrocytic anemia. Blood, 85(3), pp. 818-23.
    d'Onofrio G, et al. Simultaneous Measurement of Reticulocyte and Red Blood Cell Indices in Healthy Subjects and Patients With Microcytic and Macrocytic Anemia. Blood. 1995 Feb 1;85(3):818-23. PubMed PMID: 7833482.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Simultaneous measurement of reticulocyte and red blood cell indices in healthy subjects and patients with microcytic and macrocytic anemia. AU - d'Onofrio,G, AU - Chirillo,R, AU - Zini,G, AU - Caenaro,G, AU - Tommasi,M, AU - Micciulli,G, PY - 1995/2/1/pubmed PY - 1995/2/1/medline PY - 1995/2/1/entrez SP - 818 EP - 23 JF - Blood JO - Blood VL - 85 IS - 3 N2 - Using the new Bayer H*3 hematology analyzer (Leverkusen, Germany), we have determined red blood cell and reticulocyte indices in 64 healthy subjects, in patients with microcytosis due to iron deficiency (58 patients) and heterozygous beta-thalassemia (40 patients), and in patients with macrocytosis (28 patients). We found in all cases that reticulocytes were larger than mature red cells by 24% to 35%, with a hemoglobin concentration 16% to 25% lower and a similar hemoglobin content. The correlation between red cell and reticulocyte indices was strikingly tight (r = .928 for volume, r = .929 for hemoglobin concentration, r = .972 for hemoglobin content) in all four groups, regardless of red blood cell size. The ratio of reticulocyte to red blood cell mean corpuscolar volume (MCV ratio) was constantly above 1. Inversion of the MCV ratio was observed only in four patients. It was always abrupt and transitory and was associated with erythropoietic changes leading to the production of red blood cells of a different volume (treatment of megaloblastic anemia, functional iron deficiency, bone marrow transplantation). In two cases of marrow transplantation, reticulocyte volume fell during the aplastic phase after conditioning chemotherapy and then rapidly increased up to values higher than before; this production of macroreticulocytes was the earliest sign of engraftment. SN - 0006-4971 UR - https://www.unboundmedicine.com/medline/citation/7833482/Simultaneous_measurement_of_reticulocyte_and_red_blood_cell_indices_in_healthy_subjects_and_patients_with_microcytic_and_macrocytic_anemia_ L2 - http://www.bloodjournal.org/cgi/pmidlookup?view=long&pmid=7833482 DB - PRIME DP - Unbound Medicine ER -