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Lipid content of microparticulate (1-->3)-beta-D-glucan isolated from Saccharomyces cerevisiae.
Microbios. 1994; 79(321):253-61.M

Abstract

The preparation of pharmaceutical grade (1-->3)-beta-D-glucans from Saccharomyces cerevisiae requires that the microparticulate glucans which are employed as the starting material for drug production be of the highest purity. Potential contaminants of the (1-->3)-beta-D-glucan isolation process are yeast cell wall lipids, which are frequently found in association with glucans. The lipid content of yeast-derived (1-->3)-beta-D-glucan during various stages of the isolation process by methyl esterification and total ion gas chromatography and mass spectrometry (GC-MS) was examined. Following sequential alkaline and acid hydrolyses, the total lipid content of (1-->3)-beta-D-glucan was 100.4 nmol/mg with 9-cis-C 18:1 fatty acid accounting for 45.3 nmol/mg glucan (45%). Following ethanol extraction of the (1-->3)-beta-D-glucan, lipid was not detected. Indeed, fatty acid methyl esters were not detectable by total ion GC-MS in all twelve samples investigated. These data demonstrate that the isolation process for yeast-derived (1-->3)-beta-D-glucan effectively reduces contamination by yeast cell wall lipids.

Authors+Show Affiliations

Müller A
Department of Surgery, James H. Quillen College of Medicine, East Tennessee State University, Johnson City 37614.
Mayberry W
No affiliation info available
Acuff R
No affiliation info available
Thedford S
No affiliation info available
Browder W
No affiliation info available
Williams D
No affiliation info available

MeSH

Cell WallChromatography, GasEsterificationGlucansLipidsMass SpectrometrySaccharomyces cerevisiaebeta-Glucans

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

7837997

Citation

Müller, A, et al. "Lipid Content of Microparticulate (1-->3)-beta-D-glucan Isolated From Saccharomyces Cerevisiae." Microbios, vol. 79, no. 321, 1994, pp. 253-61.
Müller A, Mayberry W, Acuff R, et al. Lipid content of microparticulate (1-->3)-beta-D-glucan isolated from Saccharomyces cerevisiae. Microbios. 1994;79(321):253-61.
Müller, A., Mayberry, W., Acuff, R., Thedford, S., Browder, W., & Williams, D. (1994). Lipid content of microparticulate (1-->3)-beta-D-glucan isolated from Saccharomyces cerevisiae. Microbios, 79(321), 253-61.
Müller A, et al. Lipid Content of Microparticulate (1-->3)-beta-D-glucan Isolated From Saccharomyces Cerevisiae. Microbios. 1994;79(321):253-61. PubMed PMID: 7837997.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lipid content of microparticulate (1-->3)-beta-D-glucan isolated from Saccharomyces cerevisiae. AU - Müller,A, AU - Mayberry,W, AU - Acuff,R, AU - Thedford,S, AU - Browder,W, AU - Williams,D, PY - 1994/1/1/pubmed PY - 1994/1/1/medline PY - 1994/1/1/entrez SP - 253 EP - 61 JF - Microbios JO - Microbios VL - 79 IS - 321 N2 - The preparation of pharmaceutical grade (1-->3)-beta-D-glucans from Saccharomyces cerevisiae requires that the microparticulate glucans which are employed as the starting material for drug production be of the highest purity. Potential contaminants of the (1-->3)-beta-D-glucan isolation process are yeast cell wall lipids, which are frequently found in association with glucans. The lipid content of yeast-derived (1-->3)-beta-D-glucan during various stages of the isolation process by methyl esterification and total ion gas chromatography and mass spectrometry (GC-MS) was examined. Following sequential alkaline and acid hydrolyses, the total lipid content of (1-->3)-beta-D-glucan was 100.4 nmol/mg with 9-cis-C 18:1 fatty acid accounting for 45.3 nmol/mg glucan (45%). Following ethanol extraction of the (1-->3)-beta-D-glucan, lipid was not detected. Indeed, fatty acid methyl esters were not detectable by total ion GC-MS in all twelve samples investigated. These data demonstrate that the isolation process for yeast-derived (1-->3)-beta-D-glucan effectively reduces contamination by yeast cell wall lipids. SN - 0026-2633 UR - https://www.unboundmedicine.com/medline/citation/7837997/Lipid_content_of_microparticulate__1__>3__beta_D_glucan_isolated_from_Saccharomyces_cerevisiae_ DB - PRIME DP - Unbound Medicine ER -