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Effects of oxidants and antioxidants on nuclear factor kappa B activation in three different cell lines: evidence against a universal hypothesis involving oxygen radicals.
Biochim Biophys Acta. 1995 Jan 25; 1260(2):167-75.BB

Abstract

A model for NF kappa B activation involving reactive oxygen intermediates has recently been proposed. We have explored this model in three cell lines, Jurkat T cells, EL4.NOB-1 T cells and KB epidermal cells using hydrogen peroxide and two physiological activators of NF kappa B, interleukin-1 (IL1) and tumor necrosis factor (TNF) as stimuli. In agreement with earlier studies hydrogen peroxide activated NF kappa B in Jurkat, although only at much higher concentrations (10 mM) than those previously reported. However, hydrogen peroxide failed to activate in the two other cell lines under a range of conditions. Similarly, N-acetylcysteine only proved inhibitory in hydrogen peroxide and TNF treated Jurkat and failed to inhibit IL1 and TNF-activated NF kappa B in EL4.NOB-1 and KB cells respectively. N-Acetylcysteine inhibited IL1-induced interleukin-2 in EL4, however, demonstrating that N-acetylcysteine was biologically active. These results suggest that the reactive oxygen model of NF kappa B activation may be cell-type restricted. In contrast to the results with N-acetylcysteine, the antioxidant and metal chelator, pyrolidine dithiocarbamate (PDTC) inhibited NF kappa B activation, although these effects may be unrelated to any antioxidant properties. PDTC also inhibited IL1-induced interleukin-2. Finally, studies with the pro-oxidant diamide showed that this could not activate NF kappa B in any of the cells and in contrast proved inhibitory. The results from this study therefore suggest that the reactive oxygen model of NF kappa B activation may be restricted to certain cell types and that the presence of such a system is not required for the activation of NF kappa B by IL1 and TNF.

Authors+Show Affiliations

Biochemistry Department, Trinity College, Dublin, Ireland.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7841193

Citation

Brennan, P, and L A. O'Neill. "Effects of Oxidants and Antioxidants On Nuclear Factor Kappa B Activation in Three Different Cell Lines: Evidence Against a Universal Hypothesis Involving Oxygen Radicals." Biochimica Et Biophysica Acta, vol. 1260, no. 2, 1995, pp. 167-75.
Brennan P, O'Neill LA. Effects of oxidants and antioxidants on nuclear factor kappa B activation in three different cell lines: evidence against a universal hypothesis involving oxygen radicals. Biochim Biophys Acta. 1995;1260(2):167-75.
Brennan, P., & O'Neill, L. A. (1995). Effects of oxidants and antioxidants on nuclear factor kappa B activation in three different cell lines: evidence against a universal hypothesis involving oxygen radicals. Biochimica Et Biophysica Acta, 1260(2), 167-75.
Brennan P, O'Neill LA. Effects of Oxidants and Antioxidants On Nuclear Factor Kappa B Activation in Three Different Cell Lines: Evidence Against a Universal Hypothesis Involving Oxygen Radicals. Biochim Biophys Acta. 1995 Jan 25;1260(2):167-75. PubMed PMID: 7841193.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of oxidants and antioxidants on nuclear factor kappa B activation in three different cell lines: evidence against a universal hypothesis involving oxygen radicals. AU - Brennan,P, AU - O'Neill,L A, PY - 1995/1/25/pubmed PY - 1995/1/25/medline PY - 1995/1/25/entrez SP - 167 EP - 75 JF - Biochimica et biophysica acta JO - Biochim Biophys Acta VL - 1260 IS - 2 N2 - A model for NF kappa B activation involving reactive oxygen intermediates has recently been proposed. We have explored this model in three cell lines, Jurkat T cells, EL4.NOB-1 T cells and KB epidermal cells using hydrogen peroxide and two physiological activators of NF kappa B, interleukin-1 (IL1) and tumor necrosis factor (TNF) as stimuli. In agreement with earlier studies hydrogen peroxide activated NF kappa B in Jurkat, although only at much higher concentrations (10 mM) than those previously reported. However, hydrogen peroxide failed to activate in the two other cell lines under a range of conditions. Similarly, N-acetylcysteine only proved inhibitory in hydrogen peroxide and TNF treated Jurkat and failed to inhibit IL1 and TNF-activated NF kappa B in EL4.NOB-1 and KB cells respectively. N-Acetylcysteine inhibited IL1-induced interleukin-2 in EL4, however, demonstrating that N-acetylcysteine was biologically active. These results suggest that the reactive oxygen model of NF kappa B activation may be cell-type restricted. In contrast to the results with N-acetylcysteine, the antioxidant and metal chelator, pyrolidine dithiocarbamate (PDTC) inhibited NF kappa B activation, although these effects may be unrelated to any antioxidant properties. PDTC also inhibited IL1-induced interleukin-2. Finally, studies with the pro-oxidant diamide showed that this could not activate NF kappa B in any of the cells and in contrast proved inhibitory. The results from this study therefore suggest that the reactive oxygen model of NF kappa B activation may be restricted to certain cell types and that the presence of such a system is not required for the activation of NF kappa B by IL1 and TNF. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/7841193/Effects_of_oxidants_and_antioxidants_on_nuclear_factor_kappa_B_activation_in_three_different_cell_lines:_evidence_against_a_universal_hypothesis_involving_oxygen_radicals_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0167-4781(94)00186-7 DB - PRIME DP - Unbound Medicine ER -