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Selective accumulation of the X transcript of hepatitis B virus in patients negative for hepatitis B surface antigen with hepatocellular carcinoma.
Hepatology. 1995 Feb; 21(2):313-21.Hep

Abstract

In HBsAg-negative patients with hepatocellular carcinoma (HCC), hepatitis B virus (HBV) genomes are present at a low copy number per cell, and the role of HBV in liver transformation is still unclear. We have mapped by polymerase chain reaction (PCR) the HBV genome in 19 HBsAg-negative tumorous and 9 corresponding nontumorous tissues and evaluated, by RT-PCR, the presence of HBV S, X, and C transcripts in the tumorous and nontumorous tissue of nine HBsAg-negative and, for comparison, six HBsAg-positive patients. Disrupted, presumably integrated, HBV genomes were detected by PCR in 10 of 19 tumorous tissues and in only one of nine nontumorous tissues. Significant accumulation of viral RNAs containing X but not C or S sequences was shown in 7/9 tumors and 7/8 nontumorous tissues from HBsAg-negative patients. In contrast, viral RNAs revealed by X-as well as by S- and C-specific primers were detected in five of six tumors and in six of six nontumorous tissues from HBsAg-positive patients. In conclusion, our results suggest the frequent integration of the HBV genome and the accumulation of X-related RNAs in HCCs developing in HBsAg-negative patients. This finding is consistent with a role, in these cases, for the potentially transforming X protein.

Authors+Show Affiliations

INSERM U370, CHU Necker, Paris, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7843699

Citation

Paterlini, P, et al. "Selective Accumulation of the X Transcript of Hepatitis B Virus in Patients Negative for Hepatitis B Surface Antigen With Hepatocellular Carcinoma." Hepatology (Baltimore, Md.), vol. 21, no. 2, 1995, pp. 313-21.
Paterlini P, Poussin K, Kew M, et al. Selective accumulation of the X transcript of hepatitis B virus in patients negative for hepatitis B surface antigen with hepatocellular carcinoma. Hepatology. 1995;21(2):313-21.
Paterlini, P., Poussin, K., Kew, M., Franco, D., & Brechot, C. (1995). Selective accumulation of the X transcript of hepatitis B virus in patients negative for hepatitis B surface antigen with hepatocellular carcinoma. Hepatology (Baltimore, Md.), 21(2), 313-21.
Paterlini P, et al. Selective Accumulation of the X Transcript of Hepatitis B Virus in Patients Negative for Hepatitis B Surface Antigen With Hepatocellular Carcinoma. Hepatology. 1995;21(2):313-21. PubMed PMID: 7843699.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selective accumulation of the X transcript of hepatitis B virus in patients negative for hepatitis B surface antigen with hepatocellular carcinoma. AU - Paterlini,P, AU - Poussin,K, AU - Kew,M, AU - Franco,D, AU - Brechot,C, PY - 1995/2/1/pubmed PY - 1995/2/1/medline PY - 1995/2/1/entrez SP - 313 EP - 21 JF - Hepatology (Baltimore, Md.) JO - Hepatology VL - 21 IS - 2 N2 - In HBsAg-negative patients with hepatocellular carcinoma (HCC), hepatitis B virus (HBV) genomes are present at a low copy number per cell, and the role of HBV in liver transformation is still unclear. We have mapped by polymerase chain reaction (PCR) the HBV genome in 19 HBsAg-negative tumorous and 9 corresponding nontumorous tissues and evaluated, by RT-PCR, the presence of HBV S, X, and C transcripts in the tumorous and nontumorous tissue of nine HBsAg-negative and, for comparison, six HBsAg-positive patients. Disrupted, presumably integrated, HBV genomes were detected by PCR in 10 of 19 tumorous tissues and in only one of nine nontumorous tissues. Significant accumulation of viral RNAs containing X but not C or S sequences was shown in 7/9 tumors and 7/8 nontumorous tissues from HBsAg-negative patients. In contrast, viral RNAs revealed by X-as well as by S- and C-specific primers were detected in five of six tumors and in six of six nontumorous tissues from HBsAg-positive patients. In conclusion, our results suggest the frequent integration of the HBV genome and the accumulation of X-related RNAs in HCCs developing in HBsAg-negative patients. This finding is consistent with a role, in these cases, for the potentially transforming X protein. SN - 0270-9139 UR - https://www.unboundmedicine.com/medline/citation/7843699/Selective_accumulation_of_the_X_transcript_of_hepatitis_B_virus_in_patients_negative_for_hepatitis_B_surface_antigen_with_hepatocellular_carcinoma_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0270913995000449 DB - PRIME DP - Unbound Medicine ER -