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Hydrogen peroxide affects specific epithelial subpopulations in cultured rabbit lenses.
Invest Ophthalmol Vis Sci. 1995 Feb; 36(2):289-99.IO

Abstract

PURPOSE

To investigate the effect of hydrogen peroxide on the epithelial cells of cultured rabbit lenses.

METHODS

Lenses were cultured in minimum essential medium containing a single dose of 0.03, 0.1, or 0.2 mM H2O2. Three hours later the medium was replaced with peroxide-free minimum essential medium. Lenses were also treated with 0.5 mM 1,3-bis(2-chloroethyl)-1 nitrosourea (BCNU) to lower the activity of glutathione reductase and then exposed to 0.03 mM H2O2 maintained nearly constant by glucose oxidase. After H2O2 treatment, lenses were fixed and whole mounts of the epithelium were prepared or lenses were processed for electron microscopy.

RESULTS

Cells exposed to a single dose of 0.03 mM H2O2 appeared normal; 0.1 mM H2O2 was not cytotoxic. Exposure to 0.2 mM H2O2 elicited swelling in cells in the pre-equatorial region (30 minutes) followed by the formation of islands of cells in the pre-equatorial region at 1 hour. Central epithelial cells appeared normal at 1 hour, were swollen at 3 hours and dead at 24 hours. By 48 hours, dead cells were found in the pre-equatorial and central regions. Cells in the peripheral region of the epithelium did not exhibit cytotoxicity. If lenses were pretreated with BCNU and then challenged with a maintained level of 0.03 mM H2O2, cytotoxicity was induced in the central and pre-equatorial regions. Cells in the peripheral region survived BCNU-H2O2 treatment.

CONCLUSIONS

Cells in the peripheral region of cultured lenses were more resistant to H2O2 cytotoxicity than cells in the central and pre-equatorial regions. The antioxidant defense or repair systems for H2O2-induced damage do not appear to be uniformly distributed in subpopulations of the lens epithelium.

Authors+Show Affiliations

Department of Biological Sciences, Oakland University, Rochester, Michigan 48309-4401.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7843900

Citation

Reddan, J R., et al. "Hydrogen Peroxide Affects Specific Epithelial Subpopulations in Cultured Rabbit Lenses." Investigative Ophthalmology & Visual Science, vol. 36, no. 2, 1995, pp. 289-99.
Reddan JR, Giblin FJ, Dziedzic DC, et al. Hydrogen peroxide affects specific epithelial subpopulations in cultured rabbit lenses. Invest Ophthalmol Vis Sci. 1995;36(2):289-99.
Reddan, J. R., Giblin, F. J., Dziedzic, D. C., Wirebaugh, B. M., & Peters, J. L. (1995). Hydrogen peroxide affects specific epithelial subpopulations in cultured rabbit lenses. Investigative Ophthalmology & Visual Science, 36(2), 289-99.
Reddan JR, et al. Hydrogen Peroxide Affects Specific Epithelial Subpopulations in Cultured Rabbit Lenses. Invest Ophthalmol Vis Sci. 1995;36(2):289-99. PubMed PMID: 7843900.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hydrogen peroxide affects specific epithelial subpopulations in cultured rabbit lenses. AU - Reddan,J R, AU - Giblin,F J, AU - Dziedzic,D C, AU - Wirebaugh,B M, AU - Peters,J L, PY - 1995/2/1/pubmed PY - 1995/2/1/medline PY - 1995/2/1/entrez SP - 289 EP - 99 JF - Investigative ophthalmology & visual science JO - Invest Ophthalmol Vis Sci VL - 36 IS - 2 N2 - PURPOSE: To investigate the effect of hydrogen peroxide on the epithelial cells of cultured rabbit lenses. METHODS: Lenses were cultured in minimum essential medium containing a single dose of 0.03, 0.1, or 0.2 mM H2O2. Three hours later the medium was replaced with peroxide-free minimum essential medium. Lenses were also treated with 0.5 mM 1,3-bis(2-chloroethyl)-1 nitrosourea (BCNU) to lower the activity of glutathione reductase and then exposed to 0.03 mM H2O2 maintained nearly constant by glucose oxidase. After H2O2 treatment, lenses were fixed and whole mounts of the epithelium were prepared or lenses were processed for electron microscopy. RESULTS: Cells exposed to a single dose of 0.03 mM H2O2 appeared normal; 0.1 mM H2O2 was not cytotoxic. Exposure to 0.2 mM H2O2 elicited swelling in cells in the pre-equatorial region (30 minutes) followed by the formation of islands of cells in the pre-equatorial region at 1 hour. Central epithelial cells appeared normal at 1 hour, were swollen at 3 hours and dead at 24 hours. By 48 hours, dead cells were found in the pre-equatorial and central regions. Cells in the peripheral region of the epithelium did not exhibit cytotoxicity. If lenses were pretreated with BCNU and then challenged with a maintained level of 0.03 mM H2O2, cytotoxicity was induced in the central and pre-equatorial regions. Cells in the peripheral region survived BCNU-H2O2 treatment. CONCLUSIONS: Cells in the peripheral region of cultured lenses were more resistant to H2O2 cytotoxicity than cells in the central and pre-equatorial regions. The antioxidant defense or repair systems for H2O2-induced damage do not appear to be uniformly distributed in subpopulations of the lens epithelium. SN - 0146-0404 UR - https://www.unboundmedicine.com/medline/citation/7843900/Hydrogen_peroxide_affects_specific_epithelial_subpopulations_in_cultured_rabbit_lenses_ L2 - https://iovs.arvojournals.org/article.aspx?volume=36&issue=2&page=289 DB - PRIME DP - Unbound Medicine ER -