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Transformation of Bifidobacterium longum with pRM2, a constructed Escherichia coli-B. longum shuttle vector.
Plasmid. 1994 Sep; 32(2):208-11.P

Abstract

An Escherichia coli-Bifidobacterium longum shuttle vector, designated pRM2, was constructed by cloning a B. longum plasmid and an enterococcal spectinomycin resistance gene into a commercial E. coli vector. The plasmid was successfully introduced into B. longum cells by electroporation and into E. coli cells by both electroporation and chemical transformation.

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Authors+Show Affiliations

Missich R
Laboratory of Microbial Genetics, University of Bologna, Italy.
Sgorbati B
No affiliation info available
LeBlanc DJ
No affiliation info available

MeSH

BifidobacteriumDNA, BacterialDNA, RecombinantDrug Resistance, MicrobialElectroporationEscherichia coliGenetic VectorsSpecies SpecificitySpectinomycinTransformation, Bacterial

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7846144

Citation

Missich, R, et al. "Transformation of Bifidobacterium Longum With pRM2, a Constructed Escherichia coli-B. Longum Shuttle Vector." Plasmid, vol. 32, no. 2, 1994, pp. 208-11.
Missich R, Sgorbati B, LeBlanc DJ. Transformation of Bifidobacterium longum with pRM2, a constructed Escherichia coli-B. longum shuttle vector. Plasmid. 1994;32(2):208-11.
Missich, R., Sgorbati, B., & LeBlanc, D. J. (1994). Transformation of Bifidobacterium longum with pRM2, a constructed Escherichia coli-B. longum shuttle vector. Plasmid, 32(2), 208-11.
Missich R, Sgorbati B, LeBlanc DJ. Transformation of Bifidobacterium Longum With pRM2, a Constructed Escherichia coli-B. Longum Shuttle Vector. Plasmid. 1994;32(2):208-11. PubMed PMID: 7846144.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transformation of Bifidobacterium longum with pRM2, a constructed Escherichia coli-B. longum shuttle vector. AU - Missich,R, AU - Sgorbati,B, AU - LeBlanc,D J, PY - 1994/9/1/pubmed PY - 1994/9/1/medline PY - 1994/9/1/entrez SP - 208 EP - 11 JF - Plasmid JO - Plasmid VL - 32 IS - 2 N2 - An Escherichia coli-Bifidobacterium longum shuttle vector, designated pRM2, was constructed by cloning a B. longum plasmid and an enterococcal spectinomycin resistance gene into a commercial E. coli vector. The plasmid was successfully introduced into B. longum cells by electroporation and into E. coli cells by both electroporation and chemical transformation. SN - 0147-619X UR - https://www.unboundmedicine.com/medline/citation/7846144/Transformation_of_Bifidobacterium_longum_with_pRM2_a_constructed_Escherichia_coli_B__longum_shuttle_vector_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-619X(84)71056-0 DB - PRIME DP - Unbound Medicine ER -