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A clean-up method for analysis of trace amounts of microcystins in lake water.
Toxicon 1994; 32(10):1251-9T

Abstract

A clean-up method using high performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS) was developed to pursue trace amounts of microcystins in lake water. The method consisted of the combined usage of octadecyl silanized (ODS) silica gel and silica gel cartridges. In the first clean-up process, the retention behavior of microcystin RR on ODS silica gel cartridge was carefully observed together with microcystin LR, and 10% water-methanol was chosen as the best solvent system to elute microcystins from the ODS silica gel cartridge. Because many impurities still remained in the desired fraction from the raw water even after the clean-up with ODS silica gel, an additional clean-up process was developed using various cartridges. As a result of extensive experiments, the second clean-up process using silica gel cartridge was established, and the impurities were effectively eliminated. The present method including a tandem cartridge system allowed a precise analysis of microcystins in water samples from three different lakes at a 0.02 ppb level.

Authors+Show Affiliations

Kanagawa Prefectural Public Health Laboratories, Yokohama, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7846695

Citation

Tsuji, K, et al. "A Clean-up Method for Analysis of Trace Amounts of Microcystins in Lake Water." Toxicon : Official Journal of the International Society On Toxinology, vol. 32, no. 10, 1994, pp. 1251-9.
Tsuji K, Naito S, Kondo F, et al. A clean-up method for analysis of trace amounts of microcystins in lake water. Toxicon. 1994;32(10):1251-9.
Tsuji, K., Naito, S., Kondo, F., Watanabe, M. F., Suzuki, S., Nakazawa, H., ... Harada, K. (1994). A clean-up method for analysis of trace amounts of microcystins in lake water. Toxicon : Official Journal of the International Society On Toxinology, 32(10), pp. 1251-9.
Tsuji K, et al. A Clean-up Method for Analysis of Trace Amounts of Microcystins in Lake Water. Toxicon. 1994;32(10):1251-9. PubMed PMID: 7846695.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A clean-up method for analysis of trace amounts of microcystins in lake water. AU - Tsuji,K, AU - Naito,S, AU - Kondo,F, AU - Watanabe,M F, AU - Suzuki,S, AU - Nakazawa,H, AU - Suzuki,M, AU - Shimada,T, AU - Harada,K, PY - 1994/10/1/pubmed PY - 1994/10/1/medline PY - 1994/10/1/entrez SP - 1251 EP - 9 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 32 IS - 10 N2 - A clean-up method using high performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS) was developed to pursue trace amounts of microcystins in lake water. The method consisted of the combined usage of octadecyl silanized (ODS) silica gel and silica gel cartridges. In the first clean-up process, the retention behavior of microcystin RR on ODS silica gel cartridge was carefully observed together with microcystin LR, and 10% water-methanol was chosen as the best solvent system to elute microcystins from the ODS silica gel cartridge. Because many impurities still remained in the desired fraction from the raw water even after the clean-up with ODS silica gel, an additional clean-up process was developed using various cartridges. As a result of extensive experiments, the second clean-up process using silica gel cartridge was established, and the impurities were effectively eliminated. The present method including a tandem cartridge system allowed a precise analysis of microcystins in water samples from three different lakes at a 0.02 ppb level. SN - 0041-0101 UR - https://www.unboundmedicine.com/medline/citation/7846695/abstract/A_clean_up_method_for_analysis_of_trace_amounts_of_microcystins_in_lake_water_ L2 - https://www.lens.org/lens/search?q=citation_id:7846695 DB - PRIME DP - Unbound Medicine ER -