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Direct observation of cell wall glucans in whole cells of Saccharomyces cerevisiae by magic-angle spinning 13C-NMR.
Biopolymers. 1994 Dec; 34(12):1627-35.B

Abstract

Intact cells of Saccharomyces cerevisiae were examined as an aqueous paste by 13C-nmr spectroscopy with direct polarization and magic-angle spinning. The spectra obtained were highly resolved, showing numerous resonances in the 60-105 ppm range that were assigned to carbons of a liquid-like domain of the cell wall glucan. Assignments were confirmed by running the spectrum of S. cerevisiae in which the cell wall glucans were labeled with [13C] by feeding the cell [13C]galactose. The spectra indicate that the glucan in the cell wall of intact S. cerevisiae assumes a helical conformation and suggest that strain 17A fed with galactose preferentially incorporates the resulting glucose into beta (1-->3)-linkages.

Authors+Show Affiliations

Krainer E
Department of Chemistry, College of Staten Island, City University of New York 10314.
Stark RE
No affiliation info available
Naider F
No affiliation info available
Alagramam K
No affiliation info available
Becker JM
No affiliation info available

MeSH

Carbohydrate SequenceCarbon IsotopesCell WallGlucansMagnetic Resonance SpectroscopyMolecular Sequence DataSaccharomyces cerevisiae

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7849225

Citation

Krainer, E, et al. "Direct Observation of Cell Wall Glucans in Whole Cells of Saccharomyces Cerevisiae By Magic-angle Spinning 13C-NMR." Biopolymers, vol. 34, no. 12, 1994, pp. 1627-35.
Krainer E, Stark RE, Naider F, et al. Direct observation of cell wall glucans in whole cells of Saccharomyces cerevisiae by magic-angle spinning 13C-NMR. Biopolymers. 1994;34(12):1627-35.
Krainer, E., Stark, R. E., Naider, F., Alagramam, K., & Becker, J. M. (1994). Direct observation of cell wall glucans in whole cells of Saccharomyces cerevisiae by magic-angle spinning 13C-NMR. Biopolymers, 34(12), 1627-35.
Krainer E, et al. Direct Observation of Cell Wall Glucans in Whole Cells of Saccharomyces Cerevisiae By Magic-angle Spinning 13C-NMR. Biopolymers. 1994;34(12):1627-35. PubMed PMID: 7849225.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Direct observation of cell wall glucans in whole cells of Saccharomyces cerevisiae by magic-angle spinning 13C-NMR. AU - Krainer,E, AU - Stark,R E, AU - Naider,F, AU - Alagramam,K, AU - Becker,J M, PY - 1994/12/1/pubmed PY - 1994/12/1/medline PY - 1994/12/1/entrez SP - 1627 EP - 35 JF - Biopolymers JO - Biopolymers VL - 34 IS - 12 N2 - Intact cells of Saccharomyces cerevisiae were examined as an aqueous paste by 13C-nmr spectroscopy with direct polarization and magic-angle spinning. The spectra obtained were highly resolved, showing numerous resonances in the 60-105 ppm range that were assigned to carbons of a liquid-like domain of the cell wall glucan. Assignments were confirmed by running the spectrum of S. cerevisiae in which the cell wall glucans were labeled with [13C] by feeding the cell [13C]galactose. The spectra indicate that the glucan in the cell wall of intact S. cerevisiae assumes a helical conformation and suggest that strain 17A fed with galactose preferentially incorporates the resulting glucose into beta (1-->3)-linkages. SN - 0006-3525 UR - https://www.unboundmedicine.com/medline/citation/7849225/Direct_observation_of_cell_wall_glucans_in_whole_cells_of_Saccharomyces_cerevisiae_by_magic_angle_spinning_13C_NMR_ L2 - https://doi.org/10.1002/bip.360341207 DB - PRIME DP - Unbound Medicine ER -