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Calprotectin-like protein is related to soluble organic matrix in calcium oxalate urinary stone.
Biochem Mol Biol Int. 1994 Sep; 34(2):309-13.BM

Abstract

Soluble organic matrix was extracted with 0.1 M EDTA from calcium oxalate monohydrate urinary stone (CaOx), purified by SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes for the NH2-terminal amino acid sequence analysis. A protein at 30 kDa showed complete homology with calprotectin (20 amino acids in the NH2-terminal), so we termed this a calprotectin-like protein. Calprotectin which was extracted from human granulocytes had inhibitory activity in calcium oxalate monohydrate crystal growth in vitro. This study suggested that, calprotectin-like protein detected in CaOx is thought to be a potent inhibitor of crystal growth and may therefore be important in the etiology of CaOx formation.

Authors+Show Affiliations

Umekawa T
Department of Urology, Kinki University School of Medicine, Osaka, Japan.
Kurita T
No affiliation info available

MeSH

Amino Acid SequenceCalcium OxalateCalcium-Binding ProteinsCell Adhesion Molecules, NeuronalEdetic AcidElectrophoresis, Polyacrylamide GelHumansLeukocyte L1 Antigen ComplexMembranes, ArtificialMolecular Sequence DataPolyvinylsSequence Homology, Amino AcidUrinary Calculi

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

7849642

Citation

Umekawa, T, and T Kurita. "Calprotectin-like Protein Is Related to Soluble Organic Matrix in Calcium Oxalate Urinary Stone." Biochemistry and Molecular Biology International, vol. 34, no. 2, 1994, pp. 309-13.
Umekawa T, Kurita T. Calprotectin-like protein is related to soluble organic matrix in calcium oxalate urinary stone. Biochem Mol Biol Int. 1994;34(2):309-13.
Umekawa, T., & Kurita, T. (1994). Calprotectin-like protein is related to soluble organic matrix in calcium oxalate urinary stone. Biochemistry and Molecular Biology International, 34(2), 309-13.
Umekawa T, Kurita T. Calprotectin-like Protein Is Related to Soluble Organic Matrix in Calcium Oxalate Urinary Stone. Biochem Mol Biol Int. 1994;34(2):309-13. PubMed PMID: 7849642.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Calprotectin-like protein is related to soluble organic matrix in calcium oxalate urinary stone. AU - Umekawa,T, AU - Kurita,T, PY - 1994/9/1/pubmed PY - 1994/9/1/medline PY - 1994/9/1/entrez SP - 309 EP - 13 JF - Biochemistry and molecular biology international JO - Biochem Mol Biol Int VL - 34 IS - 2 N2 - Soluble organic matrix was extracted with 0.1 M EDTA from calcium oxalate monohydrate urinary stone (CaOx), purified by SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes for the NH2-terminal amino acid sequence analysis. A protein at 30 kDa showed complete homology with calprotectin (20 amino acids in the NH2-terminal), so we termed this a calprotectin-like protein. Calprotectin which was extracted from human granulocytes had inhibitory activity in calcium oxalate monohydrate crystal growth in vitro. This study suggested that, calprotectin-like protein detected in CaOx is thought to be a potent inhibitor of crystal growth and may therefore be important in the etiology of CaOx formation. SN - 1039-9712 UR - https://www.unboundmedicine.com/medline/citation/7849642/Calprotectin_like_protein_is_related_to_soluble_organic_matrix_in_calcium_oxalate_urinary_stone_ DB - PRIME DP - Unbound Medicine ER -