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Expression of selenium-dependent glutathione peroxidase in human breast tumor cell lines.
Cancer Res 1995; 55(4):957-62CR

Abstract

In estrogen receptor (ER)-positive breast cancer cell lines, very low expression of glutathione peroxidase-1 (GPX-1) activity and hgpx1 mRNA has been observed. Such cell lines have been used as models in studies of resistance to redox cycling anticancer drugs. In particular, large increases in GPX-1 activity levels by expression of transfected GPX-1 cDNA have been shown to confer some resistance to such drugs. It has never been determined that such low GPX-1 expression is a common feature of breast cancer. Based on previous limited surveys of breast cancer cell lines, it has been suggested that there may be an inverse correlation between ER status and GPX-1 production. Here we report the results from a larger survey of breast cancer cell lines, including six recently isolated cell lines. A near absence of hgpx1 mRNA expression was observed in 3 of 13 ER-negative cell lines; 1 of 4 ER-positive cell lines had high production of GPX-1. Both observations weaken the proposed inverse correlation between ER status and GPX-1 production. We have evidence to suggest that one cell line, COH-BR-5 (ER-negative), lacked hgpx1 gene expression prior to culture. This is based on the finding of stable hgpx1 gene expression during serial culture of ER-negative breast cancer cell lines newly isolated from malignant effusion and absence of hgpx1 mRNA expression in COH-BR-5. Expression of hgpx2 mRNA (producing GPXGI, the GI tract GPX) was detected in several long and newly established, ER-negative breast cancer cell lines. Cell lines, COH-BR-5 and MDA-MB-175, expressed only hgpx2 mRNA. The hgpx2 mRNA was detected in COH-BR-5 and COH-BR-7 at low passage number, suggesting that hgpx2 gene expression occurs in breast cancer malignant effusion. Thus, studies of the role of GPX in redox drug resistance may account for changes in hgpx2 gene expression. Phospholipid hydroperoxide GPX activity was not found to be generally elevated above normal tissue levels in newly established breast cancer-derived cell lines.

Authors+Show Affiliations

Department of Medical Oncology, City of Hope National Medical Center, Duarte, California 91010.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7850813

Citation

Esworthy, R S., et al. "Expression of Selenium-dependent Glutathione Peroxidase in Human Breast Tumor Cell Lines." Cancer Research, vol. 55, no. 4, 1995, pp. 957-62.
Esworthy RS, Baker MA, Chu FF. Expression of selenium-dependent glutathione peroxidase in human breast tumor cell lines. Cancer Res. 1995;55(4):957-62.
Esworthy, R. S., Baker, M. A., & Chu, F. F. (1995). Expression of selenium-dependent glutathione peroxidase in human breast tumor cell lines. Cancer Research, 55(4), pp. 957-62.
Esworthy RS, Baker MA, Chu FF. Expression of Selenium-dependent Glutathione Peroxidase in Human Breast Tumor Cell Lines. Cancer Res. 1995 Feb 15;55(4):957-62. PubMed PMID: 7850813.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of selenium-dependent glutathione peroxidase in human breast tumor cell lines. AU - Esworthy,R S, AU - Baker,M A, AU - Chu,F F, PY - 1995/2/15/pubmed PY - 1995/2/15/medline PY - 1995/2/15/entrez SP - 957 EP - 62 JF - Cancer research JO - Cancer Res. VL - 55 IS - 4 N2 - In estrogen receptor (ER)-positive breast cancer cell lines, very low expression of glutathione peroxidase-1 (GPX-1) activity and hgpx1 mRNA has been observed. Such cell lines have been used as models in studies of resistance to redox cycling anticancer drugs. In particular, large increases in GPX-1 activity levels by expression of transfected GPX-1 cDNA have been shown to confer some resistance to such drugs. It has never been determined that such low GPX-1 expression is a common feature of breast cancer. Based on previous limited surveys of breast cancer cell lines, it has been suggested that there may be an inverse correlation between ER status and GPX-1 production. Here we report the results from a larger survey of breast cancer cell lines, including six recently isolated cell lines. A near absence of hgpx1 mRNA expression was observed in 3 of 13 ER-negative cell lines; 1 of 4 ER-positive cell lines had high production of GPX-1. Both observations weaken the proposed inverse correlation between ER status and GPX-1 production. We have evidence to suggest that one cell line, COH-BR-5 (ER-negative), lacked hgpx1 gene expression prior to culture. This is based on the finding of stable hgpx1 gene expression during serial culture of ER-negative breast cancer cell lines newly isolated from malignant effusion and absence of hgpx1 mRNA expression in COH-BR-5. Expression of hgpx2 mRNA (producing GPXGI, the GI tract GPX) was detected in several long and newly established, ER-negative breast cancer cell lines. Cell lines, COH-BR-5 and MDA-MB-175, expressed only hgpx2 mRNA. The hgpx2 mRNA was detected in COH-BR-5 and COH-BR-7 at low passage number, suggesting that hgpx2 gene expression occurs in breast cancer malignant effusion. Thus, studies of the role of GPX in redox drug resistance may account for changes in hgpx2 gene expression. Phospholipid hydroperoxide GPX activity was not found to be generally elevated above normal tissue levels in newly established breast cancer-derived cell lines. SN - 0008-5472 UR - https://www.unboundmedicine.com/medline/citation/7850813/Expression_of_selenium_dependent_glutathione_peroxidase_in_human_breast_tumor_cell_lines_ L2 - http://cancerres.aacrjournals.org/cgi/pmidlookup?view=long&pmid=7850813 DB - PRIME DP - Unbound Medicine ER -