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Abundance, variability and chromosomal location of microsatellites in wheat.
Mol Gen Genet. 1995 Feb 06; 246(3):327-33.MG

Abstract

The potential of microsatellite sequences as genetic markers in hexaploid wheat (Triticum aestivum) was investigated with respect to their abundance, variability, chromosomal location and usefulness in related species. By screening a lambda phage library, the total number of (GA)n blocks was estimated to be 3.6 x 10(4) and the number of (GT)n blocks to be 2.3 x 10(4) per haploid wheat genome. This results in an average distance of approximately 270 kb between these two microsatellite types combined. Based on sequence analysis data from 70 isolated microsatellites, it was found that wheat microsatellites are relatively long containing up to 40 dinucleotide repeats. Of the tested primer pairs, 36% resulted in fragments with a size corresponding to the expected length of the sequenced microsatellite clone. The variability of 15 microsatellite markers was investigated on 18 wheat accessions. Significantly, more variation was detected with the microsatellite markers than with RFLP markers with, on average, 4.6 different alleles per microsatellite. The 15 PCR-amplified microsatellites were further localized on chromosome arms using cytogenetic stocks of Chinese Spring. Finally, the primers for the 15 wheat microsatellites were used for PCR amplification with rye (Secale cereale) and barley accessions (Hordeum vulgare, H. spontaneum). Amplified fragments were observed for ten primer pairs with barley DNA and for nine primer pairs with rye DNA as template. A microsatellite was found by dot blot analysis in the PCR products of barley and rye DNA for only one primer pair.

Authors+Show Affiliations

Röder MS
Institut für Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, Germany.
Plaschke J
No affiliation info available
König SU
No affiliation info available
Börner A
No affiliation info available
Sorrells ME
No affiliation info available
Tanksley SD
No affiliation info available
Ganal MW
No affiliation info available

MeSH

Base SequenceChromosome MappingDNA PrimersDNA, PlantDNA, SatelliteGenetic MarkersHordeumMolecular Sequence DataOligodeoxyribonucleotidesPolymorphism, GeneticRepetitive Sequences, Nucleic AcidSecaleSpecies SpecificityTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

7854317

Citation

Röder, M S., et al. "Abundance, Variability and Chromosomal Location of Microsatellites in Wheat." Molecular & General Genetics : MGG, vol. 246, no. 3, 1995, pp. 327-33.
Röder MS, Plaschke J, König SU, et al. Abundance, variability and chromosomal location of microsatellites in wheat. Mol Gen Genet. 1995;246(3):327-33.
Röder, M. S., Plaschke, J., König, S. U., Börner, A., Sorrells, M. E., Tanksley, S. D., & Ganal, M. W. (1995). Abundance, variability and chromosomal location of microsatellites in wheat. Molecular & General Genetics : MGG, 246(3), 327-33.
Röder MS, et al. Abundance, Variability and Chromosomal Location of Microsatellites in Wheat. Mol Gen Genet. 1995 Feb 6;246(3):327-33. PubMed PMID: 7854317.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Abundance, variability and chromosomal location of microsatellites in wheat. AU - Röder,M S, AU - Plaschke,J, AU - König,S U, AU - Börner,A, AU - Sorrells,M E, AU - Tanksley,S D, AU - Ganal,M W, PY - 1995/2/6/pubmed PY - 1995/2/6/medline PY - 1995/2/6/entrez SP - 327 EP - 33 JF - Molecular & general genetics : MGG JO - Mol Gen Genet VL - 246 IS - 3 N2 - The potential of microsatellite sequences as genetic markers in hexaploid wheat (Triticum aestivum) was investigated with respect to their abundance, variability, chromosomal location and usefulness in related species. By screening a lambda phage library, the total number of (GA)n blocks was estimated to be 3.6 x 10(4) and the number of (GT)n blocks to be 2.3 x 10(4) per haploid wheat genome. This results in an average distance of approximately 270 kb between these two microsatellite types combined. Based on sequence analysis data from 70 isolated microsatellites, it was found that wheat microsatellites are relatively long containing up to 40 dinucleotide repeats. Of the tested primer pairs, 36% resulted in fragments with a size corresponding to the expected length of the sequenced microsatellite clone. The variability of 15 microsatellite markers was investigated on 18 wheat accessions. Significantly, more variation was detected with the microsatellite markers than with RFLP markers with, on average, 4.6 different alleles per microsatellite. The 15 PCR-amplified microsatellites were further localized on chromosome arms using cytogenetic stocks of Chinese Spring. Finally, the primers for the 15 wheat microsatellites were used for PCR amplification with rye (Secale cereale) and barley accessions (Hordeum vulgare, H. spontaneum). Amplified fragments were observed for ten primer pairs with barley DNA and for nine primer pairs with rye DNA as template. A microsatellite was found by dot blot analysis in the PCR products of barley and rye DNA for only one primer pair. SN - 0026-8925 UR - https://www.unboundmedicine.com/medline/citation/7854317/Abundance_variability_and_chromosomal_location_of_microsatellites_in_wheat_ L2 - https://doi.org/10.1007/BF00288605 DB - PRIME DP - Unbound Medicine ER -