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Efficient EBV superinfection of group I Burkitt's lymphoma cells distinguishes requirements for expression of the Cp viral promoter and can activate the EBV productive cycle.
Virology. 1995 Feb 01; 206(2):866-77.V

Abstract

Group I Burkitt's lymphoma cell lines conditionally expressing the CD21 receptor for EBV infection were superinfected with EBV. The incoming EBV entered its normal program of gene expression, producing EBNA-2 and LMP-1 and activating the Cp EBNA promoter, but the endogenous virus in the BL lines was not induced to express EBNA-2 or transcribe RNA from Cp. LMP-1 was, however, expressed from the endogenous genome in response to superinfection. In a proportion of the superinfected Akata cells, the productive cycle antigen BZLF1 was induced and the ability of infecting virus to cause this was sensitive to inactivation by uv light. The results show that the restricted latent pattern of EBV gene expression observed in Group I BL cells is not a consequence of lack of appropriate transcription factor activity but results from inactivation of part of the viral genome, probably by methylation. Induction of BZLF1 in some of the cells also indicates a novel pathway for activation of the virus productive cycle.

Authors+Show Affiliations

Ludwig Institute for Cancer Research, St Mary's Hospital Medical School, London, United Kingdom.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

7856099

Citation

Evans, T J., et al. "Efficient EBV Superinfection of Group I Burkitt's Lymphoma Cells Distinguishes Requirements for Expression of the Cp Viral Promoter and Can Activate the EBV Productive Cycle." Virology, vol. 206, no. 2, 1995, pp. 866-77.
Evans TJ, Jacquemin MG, Farrell PJ. Efficient EBV superinfection of group I Burkitt's lymphoma cells distinguishes requirements for expression of the Cp viral promoter and can activate the EBV productive cycle. Virology. 1995;206(2):866-77.
Evans, T. J., Jacquemin, M. G., & Farrell, P. J. (1995). Efficient EBV superinfection of group I Burkitt's lymphoma cells distinguishes requirements for expression of the Cp viral promoter and can activate the EBV productive cycle. Virology, 206(2), 866-77.
Evans TJ, Jacquemin MG, Farrell PJ. Efficient EBV Superinfection of Group I Burkitt's Lymphoma Cells Distinguishes Requirements for Expression of the Cp Viral Promoter and Can Activate the EBV Productive Cycle. Virology. 1995 Feb 1;206(2):866-77. PubMed PMID: 7856099.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Efficient EBV superinfection of group I Burkitt's lymphoma cells distinguishes requirements for expression of the Cp viral promoter and can activate the EBV productive cycle. AU - Evans,T J, AU - Jacquemin,M G, AU - Farrell,P J, PY - 1995/2/1/pubmed PY - 1995/2/1/medline PY - 1995/2/1/entrez SP - 866 EP - 77 JF - Virology JO - Virology VL - 206 IS - 2 N2 - Group I Burkitt's lymphoma cell lines conditionally expressing the CD21 receptor for EBV infection were superinfected with EBV. The incoming EBV entered its normal program of gene expression, producing EBNA-2 and LMP-1 and activating the Cp EBNA promoter, but the endogenous virus in the BL lines was not induced to express EBNA-2 or transcribe RNA from Cp. LMP-1 was, however, expressed from the endogenous genome in response to superinfection. In a proportion of the superinfected Akata cells, the productive cycle antigen BZLF1 was induced and the ability of infecting virus to cause this was sensitive to inactivation by uv light. The results show that the restricted latent pattern of EBV gene expression observed in Group I BL cells is not a consequence of lack of appropriate transcription factor activity but results from inactivation of part of the viral genome, probably by methylation. Induction of BZLF1 in some of the cells also indicates a novel pathway for activation of the virus productive cycle. SN - 0042-6822 UR - https://www.unboundmedicine.com/medline/citation/7856099/Efficient_EBV_superinfection_of_group_I_Burkitt's_lymphoma_cells_distinguishes_requirements_for_expression_of_the_Cp_viral_promoter_and_can_activate_the_EBV_productive_cycle_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0042-6822(85)71009-4 DB - PRIME DP - Unbound Medicine ER -