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Quantitative control of the stationary phase-specific sigma factor, sigma S, in Escherichia coli: involvement of the nucleoid protein H-NS.
EMBO J. 1995 Feb 01; 14(3):594-602.EJ

Abstract

In Escherichia coli, recent intensive studies revealed that expression of a certain subset of genes is under the control of the stationary phase-specific sigma factor, sigma S, which is encoded by the rpoS gene. Since sigma S functions predominantly under certain growth conditions, its activity and/or cellular content has accordingly to be tightly controlled, however, the underlying molecular mechanism is at present unclear. We previously demonstrated that expression of the cbpA gene, encoding an analogue of the DnaJ molecular chaperone, is largely dependent on sigma S function. Here we have found that a mutational lesion of the hns gene, which encodes one of the well-characterized nucleoid proteins, H-NS, affects the cellular content of sigma S remarkably and consequently affects the expression of cbpA. Enhanced accumulation of sigma S in hns deletion cells was particularly observed in the logarithmic growth phase and was demonstrated to result from an elevated translational efficiency of rpoS mRNA and also from an increased stability of newly synthesized sigma S. Although H-NS is known to influence the transcription of a number of apparently unlinked genes on the chromosome, in this study we provide a novel instance in which H-NS is deeply implicated in post-transcriptional regulation(s) of the expression of rpoS. As to physiological relevance, it was also demonstrated that hns deletion cells exhibit an extreme thermotolerance even in the logarithmic growth phase, presumably because of the enhanced accumulation of sigma S.

Authors+Show Affiliations

Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7859747

Citation

Yamashino, T, et al. "Quantitative Control of the Stationary Phase-specific Sigma Factor, Sigma S, in Escherichia Coli: Involvement of the Nucleoid Protein H-NS." The EMBO Journal, vol. 14, no. 3, 1995, pp. 594-602.
Yamashino T, Ueguchi C, Mizuno T. Quantitative control of the stationary phase-specific sigma factor, sigma S, in Escherichia coli: involvement of the nucleoid protein H-NS. EMBO J. 1995;14(3):594-602.
Yamashino, T., Ueguchi, C., & Mizuno, T. (1995). Quantitative control of the stationary phase-specific sigma factor, sigma S, in Escherichia coli: involvement of the nucleoid protein H-NS. The EMBO Journal, 14(3), 594-602.
Yamashino T, Ueguchi C, Mizuno T. Quantitative Control of the Stationary Phase-specific Sigma Factor, Sigma S, in Escherichia Coli: Involvement of the Nucleoid Protein H-NS. EMBO J. 1995 Feb 1;14(3):594-602. PubMed PMID: 7859747.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative control of the stationary phase-specific sigma factor, sigma S, in Escherichia coli: involvement of the nucleoid protein H-NS. AU - Yamashino,T, AU - Ueguchi,C, AU - Mizuno,T, PY - 1995/2/1/pubmed PY - 1995/2/1/medline PY - 1995/2/1/entrez SP - 594 EP - 602 JF - The EMBO journal JO - EMBO J VL - 14 IS - 3 N2 - In Escherichia coli, recent intensive studies revealed that expression of a certain subset of genes is under the control of the stationary phase-specific sigma factor, sigma S, which is encoded by the rpoS gene. Since sigma S functions predominantly under certain growth conditions, its activity and/or cellular content has accordingly to be tightly controlled, however, the underlying molecular mechanism is at present unclear. We previously demonstrated that expression of the cbpA gene, encoding an analogue of the DnaJ molecular chaperone, is largely dependent on sigma S function. Here we have found that a mutational lesion of the hns gene, which encodes one of the well-characterized nucleoid proteins, H-NS, affects the cellular content of sigma S remarkably and consequently affects the expression of cbpA. Enhanced accumulation of sigma S in hns deletion cells was particularly observed in the logarithmic growth phase and was demonstrated to result from an elevated translational efficiency of rpoS mRNA and also from an increased stability of newly synthesized sigma S. Although H-NS is known to influence the transcription of a number of apparently unlinked genes on the chromosome, in this study we provide a novel instance in which H-NS is deeply implicated in post-transcriptional regulation(s) of the expression of rpoS. As to physiological relevance, it was also demonstrated that hns deletion cells exhibit an extreme thermotolerance even in the logarithmic growth phase, presumably because of the enhanced accumulation of sigma S. SN - 0261-4189 UR - https://www.unboundmedicine.com/medline/citation/7859747/Quantitative_control_of_the_stationary_phase_specific_sigma_factor_sigma_S_in_Escherichia_coli:_involvement_of_the_nucleoid_protein_H_NS_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0261-4189&date=1995&volume=14&issue=3&spage=594 DB - PRIME DP - Unbound Medicine ER -