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Human oesophagus: a convenient antigenic substrate for the determination of anti-endomysium antibodies in the serological diagnosis of coeliac disease.
Eur J Gastroenterol Hepatol 1995; 7(1):37-40EJ

Abstract

OBJECTIVE

Immunoglobulin (Ig) A-class anti-endomysium antibodies are superior to other current antibody tests for detecting coeliac disease. We aimed to evaluate the suitability of human oesophagus for the determination of anti-endomysium antibodies.

DESIGN

The specificity of monkey and human oesophageal tissue as antigenic substrate were compared using indirect immunofluorescence analysis.

PATIENTS AND METHODS

Overall, 159 individuals were studied: 56 patients with biopsy-proven coeliac disease (39 with active disease) and 103 controls. The patients' IgA-class anti-endomysium antibodies were compared using unfixed cryostat sections of human and monkey oesophagus. Indirect immunofluorescence analysis was performed with an initial serum sample dilution of 1:5, and if positive, the highest dilution yielding a positive reaction was reported.

RESULTS

The anti-endomysium antibody test was positive in 38 out of 39 patients with active coeliac disease using monkey oesophagus (sensitivity 97%) and in all 39 patients with active coeliac disease using human oesophagus (sensitivity 100%). Ten out of 17 coeliac patients on a gluten-free diet had positive anti-endomysium antibodies using monkey oesophagus and 12 using human oesophagus as the antigenic substrate. This test was negative in all 103 controls using both substrates.

CONCLUSIONS

Our study shows that human oesophageal tissue can be used instead of monkey tissue for determining anti-endomysium antibodies. Human tissue is a more sensitive antigenic substrate than monkey oesophagus and can be used to determine low titres of antibodies. Improving the diagnostic sensitivity of the anti-endomysium antibody test would make an important contribution to screening for coeliac disease.

Authors+Show Affiliations

Department of Pediatrics, University of Tartu, Estonia.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7866808

Citation

Uibo, O, et al. "Human Oesophagus: a Convenient Antigenic Substrate for the Determination of Anti-endomysium Antibodies in the Serological Diagnosis of Coeliac Disease." European Journal of Gastroenterology & Hepatology, vol. 7, no. 1, 1995, pp. 37-40.
Uibo O, Lambrechts A, Mascart-Lemone F. Human oesophagus: a convenient antigenic substrate for the determination of anti-endomysium antibodies in the serological diagnosis of coeliac disease. Eur J Gastroenterol Hepatol. 1995;7(1):37-40.
Uibo, O., Lambrechts, A., & Mascart-Lemone, F. (1995). Human oesophagus: a convenient antigenic substrate for the determination of anti-endomysium antibodies in the serological diagnosis of coeliac disease. European Journal of Gastroenterology & Hepatology, 7(1), pp. 37-40.
Uibo O, Lambrechts A, Mascart-Lemone F. Human Oesophagus: a Convenient Antigenic Substrate for the Determination of Anti-endomysium Antibodies in the Serological Diagnosis of Coeliac Disease. Eur J Gastroenterol Hepatol. 1995;7(1):37-40. PubMed PMID: 7866808.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human oesophagus: a convenient antigenic substrate for the determination of anti-endomysium antibodies in the serological diagnosis of coeliac disease. AU - Uibo,O, AU - Lambrechts,A, AU - Mascart-Lemone,F, PY - 1995/1/1/pubmed PY - 1995/1/1/medline PY - 1995/1/1/entrez SP - 37 EP - 40 JF - European journal of gastroenterology & hepatology JO - Eur J Gastroenterol Hepatol VL - 7 IS - 1 N2 - OBJECTIVE: Immunoglobulin (Ig) A-class anti-endomysium antibodies are superior to other current antibody tests for detecting coeliac disease. We aimed to evaluate the suitability of human oesophagus for the determination of anti-endomysium antibodies. DESIGN: The specificity of monkey and human oesophageal tissue as antigenic substrate were compared using indirect immunofluorescence analysis. PATIENTS AND METHODS: Overall, 159 individuals were studied: 56 patients with biopsy-proven coeliac disease (39 with active disease) and 103 controls. The patients' IgA-class anti-endomysium antibodies were compared using unfixed cryostat sections of human and monkey oesophagus. Indirect immunofluorescence analysis was performed with an initial serum sample dilution of 1:5, and if positive, the highest dilution yielding a positive reaction was reported. RESULTS: The anti-endomysium antibody test was positive in 38 out of 39 patients with active coeliac disease using monkey oesophagus (sensitivity 97%) and in all 39 patients with active coeliac disease using human oesophagus (sensitivity 100%). Ten out of 17 coeliac patients on a gluten-free diet had positive anti-endomysium antibodies using monkey oesophagus and 12 using human oesophagus as the antigenic substrate. This test was negative in all 103 controls using both substrates. CONCLUSIONS: Our study shows that human oesophageal tissue can be used instead of monkey tissue for determining anti-endomysium antibodies. Human tissue is a more sensitive antigenic substrate than monkey oesophagus and can be used to determine low titres of antibodies. Improving the diagnostic sensitivity of the anti-endomysium antibody test would make an important contribution to screening for coeliac disease. SN - 0954-691X UR - https://www.unboundmedicine.com/medline/citation/7866808/Human_oesophagus:_a_convenient_antigenic_substrate_for_the_determination_of_anti_endomysium_antibodies_in_the_serological_diagnosis_of_coeliac_disease_ L2 - https://medlineplus.gov/celiacdisease.html DB - PRIME DP - Unbound Medicine ER -