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Flow cytometric determination of leukemia-associated marker combinations for the study of minimal residual disease.
Neoplasma. 1994; 41(6):305-13.N

Abstract

To study the minimal residual disease in acute leukemia patients we used some marker combinations related either to the simultaneous surface membrane and cytoplasmic marker expression, or to the expression of atypical marker combinations, that are absent or extremely rare in normal hematopoietic cells. We investigated to which extent flow cytometric analysis of leukemia-associated marker combination may contribute to sensitive follow-up in patients with acute leukemia. For this purpose dilution experiments were performed, in which artificial mixtures of normal peripheral blood lymphocytes and leukemia cells from a patient with leukemia-associated phenotype were prepared and analyzed for double positive cells. Our results showed that the sensitivity of double color immunofluorescence assay was 3 in 10(4) cells. Sequential studies of residual disease were evaluated in five acute leukemia patients with leukemia-associated markers combinations at diagnosis. In three of them morphologic relapse was preceded by the immunologic detection of small amounts of leukemia cells, while in two other cases, in which no double positive cells for leukemia-associated markers were found, patients are still in hematologic remission. This approach to the study of minimal residual disease could be valuable in monitoring the efficiency of chemotherapy, as well as in evaluating the quality control of bone marrow before autografting. Furthermore, flow cytometric approach can efficiently complete other methods, which are used for more exact definition of remission in acute leukemia patients.

Authors+Show Affiliations

Cancer Research Institute, Slovak Academy of Sciences, Bratislava.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7870213

Citation

Babusíková, O, et al. "Flow Cytometric Determination of Leukemia-associated Marker Combinations for the Study of Minimal Residual Disease." Neoplasma, vol. 41, no. 6, 1994, pp. 305-13.
Babusíková O, Glasová M, Kusenda J, et al. Flow cytometric determination of leukemia-associated marker combinations for the study of minimal residual disease. Neoplasma. 1994;41(6):305-13.
Babusíková, O., Glasová, M., Kusenda, J., Koníková, E., & Mésárosová, A. (1994). Flow cytometric determination of leukemia-associated marker combinations for the study of minimal residual disease. Neoplasma, 41(6), 305-13.
Babusíková O, et al. Flow Cytometric Determination of Leukemia-associated Marker Combinations for the Study of Minimal Residual Disease. Neoplasma. 1994;41(6):305-13. PubMed PMID: 7870213.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Flow cytometric determination of leukemia-associated marker combinations for the study of minimal residual disease. AU - Babusíková,O, AU - Glasová,M, AU - Kusenda,J, AU - Koníková,E, AU - Mésárosová,A, PY - 1994/1/1/pubmed PY - 1994/1/1/medline PY - 1994/1/1/entrez SP - 305 EP - 13 JF - Neoplasma JO - Neoplasma VL - 41 IS - 6 N2 - To study the minimal residual disease in acute leukemia patients we used some marker combinations related either to the simultaneous surface membrane and cytoplasmic marker expression, or to the expression of atypical marker combinations, that are absent or extremely rare in normal hematopoietic cells. We investigated to which extent flow cytometric analysis of leukemia-associated marker combination may contribute to sensitive follow-up in patients with acute leukemia. For this purpose dilution experiments were performed, in which artificial mixtures of normal peripheral blood lymphocytes and leukemia cells from a patient with leukemia-associated phenotype were prepared and analyzed for double positive cells. Our results showed that the sensitivity of double color immunofluorescence assay was 3 in 10(4) cells. Sequential studies of residual disease were evaluated in five acute leukemia patients with leukemia-associated markers combinations at diagnosis. In three of them morphologic relapse was preceded by the immunologic detection of small amounts of leukemia cells, while in two other cases, in which no double positive cells for leukemia-associated markers were found, patients are still in hematologic remission. This approach to the study of minimal residual disease could be valuable in monitoring the efficiency of chemotherapy, as well as in evaluating the quality control of bone marrow before autografting. Furthermore, flow cytometric approach can efficiently complete other methods, which are used for more exact definition of remission in acute leukemia patients. SN - 0028-2685 UR - https://www.unboundmedicine.com/medline/citation/7870213/Flow_cytometric_determination_of_leukemia_associated_marker_combinations_for_the_study_of_minimal_residual_disease_ DB - PRIME DP - Unbound Medicine ER -