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Cross-linked chitosan microspheres as carriers for prolonged delivery of macromolecular drugs.
J Biomater Sci Polym Ed. 1994; 6(7):621-32.JB

Abstract

Bovine serum albumin (BSA) and diphtheria toxoid (DT) were loaded by passive absorption from aqueous solutions into preformed glutaraldehyde cross-linked chitosan microspheres. In vitro release of BSA under sink conditions at 37 degrees C showed that even though there was a large burst effect, there was a more or less steady increase with time thereafter for several days. Coating the BSA-loaded particles with paraffin oil or with a polymer, such as polylactic acid, modulated drug release. After the initial burst from PLA coated particles, the release rate increased with time for nearly 2 months. Preliminary immunogenicity studies on Wistar rats using DT loaded chitosan spheres showed that the antibody titres were fairly constant over a 5-month period, although very low compared to DT given on alum as control. Histological studies of placebo microspheres intramuscularly injected into rats demonstrated their tissue compatibility. Biodegradation was not complete in 6 months demonstrating the potential of cross-linked chitosan spheres as a long-acting drug delivery vehicle. The study demonstrated the possibility of incorporating biological macromolecules which are very sensitive to organic solvents, pH, temperature, ultrasound, etc. by a passive absorption technique to degradable biopolymer matrices thereby preserving their biological integrity. It is also shown that drugs passively absorbed into such matrices by taking advantage of their swelling behaviour need not necessarily be released completely in the initial 'burst' and a sustained release may be possible for macromolecules thus incorporated.

Authors+Show Affiliations

Polymer Chemistry Division, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, India.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7873513

Citation

Jameela, S R., et al. "Cross-linked Chitosan Microspheres as Carriers for Prolonged Delivery of Macromolecular Drugs." Journal of Biomaterials Science. Polymer Edition, vol. 6, no. 7, 1994, pp. 621-32.
Jameela SR, Misra A, Jayakrishnan A. Cross-linked chitosan microspheres as carriers for prolonged delivery of macromolecular drugs. J Biomater Sci Polym Ed. 1994;6(7):621-32.
Jameela, S. R., Misra, A., & Jayakrishnan, A. (1994). Cross-linked chitosan microspheres as carriers for prolonged delivery of macromolecular drugs. Journal of Biomaterials Science. Polymer Edition, 6(7), 621-32.
Jameela SR, Misra A, Jayakrishnan A. Cross-linked Chitosan Microspheres as Carriers for Prolonged Delivery of Macromolecular Drugs. J Biomater Sci Polym Ed. 1994;6(7):621-32. PubMed PMID: 7873513.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cross-linked chitosan microspheres as carriers for prolonged delivery of macromolecular drugs. AU - Jameela,S R, AU - Misra,A, AU - Jayakrishnan,A, PY - 1994/1/1/pubmed PY - 1994/1/1/medline PY - 1994/1/1/entrez SP - 621 EP - 32 JF - Journal of biomaterials science. Polymer edition JO - J Biomater Sci Polym Ed VL - 6 IS - 7 N2 - Bovine serum albumin (BSA) and diphtheria toxoid (DT) were loaded by passive absorption from aqueous solutions into preformed glutaraldehyde cross-linked chitosan microspheres. In vitro release of BSA under sink conditions at 37 degrees C showed that even though there was a large burst effect, there was a more or less steady increase with time thereafter for several days. Coating the BSA-loaded particles with paraffin oil or with a polymer, such as polylactic acid, modulated drug release. After the initial burst from PLA coated particles, the release rate increased with time for nearly 2 months. Preliminary immunogenicity studies on Wistar rats using DT loaded chitosan spheres showed that the antibody titres were fairly constant over a 5-month period, although very low compared to DT given on alum as control. Histological studies of placebo microspheres intramuscularly injected into rats demonstrated their tissue compatibility. Biodegradation was not complete in 6 months demonstrating the potential of cross-linked chitosan spheres as a long-acting drug delivery vehicle. The study demonstrated the possibility of incorporating biological macromolecules which are very sensitive to organic solvents, pH, temperature, ultrasound, etc. by a passive absorption technique to degradable biopolymer matrices thereby preserving their biological integrity. It is also shown that drugs passively absorbed into such matrices by taking advantage of their swelling behaviour need not necessarily be released completely in the initial 'burst' and a sustained release may be possible for macromolecules thus incorporated. SN - 0920-5063 UR - https://www.unboundmedicine.com/medline/citation/7873513/Cross_linked_chitosan_microspheres_as_carriers_for_prolonged_delivery_of_macromolecular_drugs_ L2 - http://www.tandfonline.com/doi/full/10.1163/156856294x00563 DB - PRIME DP - Unbound Medicine ER -