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[Gene diagnosis of pancreatic adenocarcinoma].
Zhonghua Bing Li Xue Za Zhi. 1994 Oct; 23(5):270-3.ZB

Abstract

Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to analyze c-Ki-ras codon 12 mutation in 27 fine needle aspiration biopsy (FNAB) specimens of the pancreas and its adjacent organs for the diagnosis of pancreatic adenocarcinoma. C-Ki-ras codon 12 mutation was present in 14 out of 15 cases of pancreatic adenocarcinoma, the positive rate was 93.33% (14/15); whereas no mutation was detected in those obtained from 12 patients with chronic pancreatitis, pancreatic cyst, gallbladder carcinoma, carcinoma of ampulla of Vater and gastric lymphoma. The results of this study verifies the PCR-RFLP technique as a practical, sensitive, rapid and reliable method for the detection of c-Ki-ras codon 12 mutation in the diagnosis of pancreatic adenocarcinoma.

Authors+Show Affiliations

Department of Pathology, PUMC Hospital, Beijing.No affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

7874757

Citation

Wang, Z Y., et al. "[Gene Diagnosis of Pancreatic Adenocarcinoma]." Zhonghua Bing Li Xue Za Zhi = Chinese Journal of Pathology, vol. 23, no. 5, 1994, pp. 270-3.
Wang ZY, Liu TH, Cui QC. [Gene diagnosis of pancreatic adenocarcinoma]. Zhonghua Bing Li Xue Za Zhi. 1994;23(5):270-3.
Wang, Z. Y., Liu, T. H., & Cui, Q. C. (1994). [Gene diagnosis of pancreatic adenocarcinoma]. Zhonghua Bing Li Xue Za Zhi = Chinese Journal of Pathology, 23(5), 270-3.
Wang ZY, Liu TH, Cui QC. [Gene Diagnosis of Pancreatic Adenocarcinoma]. Zhonghua Bing Li Xue Za Zhi. 1994;23(5):270-3. PubMed PMID: 7874757.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Gene diagnosis of pancreatic adenocarcinoma]. AU - Wang,Z Y, AU - Liu,T H, AU - Cui,Q C, PY - 1994/10/1/pubmed PY - 1994/10/1/medline PY - 1994/10/1/entrez SP - 270 EP - 3 JF - Zhonghua bing li xue za zhi = Chinese journal of pathology JO - Zhonghua Bing Li Xue Za Zhi VL - 23 IS - 5 N2 - Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to analyze c-Ki-ras codon 12 mutation in 27 fine needle aspiration biopsy (FNAB) specimens of the pancreas and its adjacent organs for the diagnosis of pancreatic adenocarcinoma. C-Ki-ras codon 12 mutation was present in 14 out of 15 cases of pancreatic adenocarcinoma, the positive rate was 93.33% (14/15); whereas no mutation was detected in those obtained from 12 patients with chronic pancreatitis, pancreatic cyst, gallbladder carcinoma, carcinoma of ampulla of Vater and gastric lymphoma. The results of this study verifies the PCR-RFLP technique as a practical, sensitive, rapid and reliable method for the detection of c-Ki-ras codon 12 mutation in the diagnosis of pancreatic adenocarcinoma. SN - 0529-5807 UR - https://www.unboundmedicine.com/medline/citation/7874757/[Gene_diagnosis_of_pancreatic_adenocarcinoma]_ L2 - https://medlineplus.gov/pancreaticcancer.html DB - PRIME DP - Unbound Medicine ER -