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Calibration problems of subcutaneous glucosensors when applied "in-situ" in man.

Abstract

Continuous glucose monitoring is the conditio sine qua non to achieve total automation in glucose-controlled insulin-delivery. Several types of glucosensors have been designed according to the enzyme-amperometric method to measure the glucose in different human compartments. However, problems such as long-term stability and calibration prevent this technique being put into practice. A feasible method is needed to calibrate the glucosensor and at the same time should be accepted by the patients. To achieve calibration we determined the absolute tissue glucose, as well as the microdialysis recovery in-vivo, in healthy subjects under normal conditions and during a hyperglycaemic clamp by applying a device based on the recirculation of phosphate buffer saline in a microdialysis probe implanted in the s.c. adipose tissue. The first experiments carried out were promising and encouraging, but further investigations are still needed to favour an ideal "before implantation, all in-vitro" method to calibrate a s.c. glucosensor.

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    MeSH

    Biosensing Techniques
    Blood Glucose
    Calibration
    Female
    Glucose
    Glucose Clamp Technique
    Humans
    Male
    Microdialysis
    Quality Control
    Skin

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    7875646

    Citation

    TY - JOUR T1 - Calibration problems of subcutaneous glucosensors when applied "in-situ" in man. AU - Sternberg,F, AU - Meyerhoff,C, AU - Mennel,F J, AU - Hoss,U, AU - Mayer,H, AU - Bischof,F, AU - Pfeiffer,E F, PY - 1994/11/1/pubmed PY - 1994/11/1/medline PY - 1994/11/1/entrez SP - 523 EP - 5 JF - Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et métabolisme JO - Horm. Metab. Res. VL - 26 IS - 11 N2 - Continuous glucose monitoring is the conditio sine qua non to achieve total automation in glucose-controlled insulin-delivery. Several types of glucosensors have been designed according to the enzyme-amperometric method to measure the glucose in different human compartments. However, problems such as long-term stability and calibration prevent this technique being put into practice. A feasible method is needed to calibrate the glucosensor and at the same time should be accepted by the patients. To achieve calibration we determined the absolute tissue glucose, as well as the microdialysis recovery in-vivo, in healthy subjects under normal conditions and during a hyperglycaemic clamp by applying a device based on the recirculation of phosphate buffer saline in a microdialysis probe implanted in the s.c. adipose tissue. The first experiments carried out were promising and encouraging, but further investigations are still needed to favour an ideal "before implantation, all in-vitro" method to calibrate a s.c. glucosensor. SN - 0018-5043 UR - https://www.unboundmedicine.com/medline/citation/7875646/abstract/Calibration_problems_of_subcutaneous_glucosensors_when_applied_"in_situ"_in_man_ L2 - http://www.thieme-connect.com/DOI/DOI?10.1055/s-2007-1001748 ER -