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A model for copy number control of the plasmid R1.
J Mol Biol 1995; 246(4):472-85JM

Abstract

A new model for copy number control of the plasmid R1 has been developed. It takes into account that initiation of replication of R1 requires a large number of cis-acting proteins (RepA). The theory explains how plasmid production rates respond to shifts in external conditions. It predicts the observed "eclipse" times between two plasmid duplications as well as the replication time for "runaway" plasmids lacking the antisense inhibitor CopA. The model also describes how the use of many cis-acting RepAs can lead to a tight coupling between cell and plasmid cycles that minimizes the rate of the plasmid loss. The results may be used as a guideline for construction of low copy number plasmids with high maintenance stability.

Authors+Show Affiliations

Department of Molecular Biology, BMC, Uppsala, Sweden.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7877168

Citation

Ehrenberg, M, and A Sverredal. "A Model for Copy Number Control of the Plasmid R1." Journal of Molecular Biology, vol. 246, no. 4, 1995, pp. 472-85.
Ehrenberg M, Sverredal A. A model for copy number control of the plasmid R1. J Mol Biol. 1995;246(4):472-85.
Ehrenberg, M., & Sverredal, A. (1995). A model for copy number control of the plasmid R1. Journal of Molecular Biology, 246(4), pp. 472-85.
Ehrenberg M, Sverredal A. A Model for Copy Number Control of the Plasmid R1. J Mol Biol. 1995 Mar 3;246(4):472-85. PubMed PMID: 7877168.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A model for copy number control of the plasmid R1. AU - Ehrenberg,M, AU - Sverredal,A, PY - 1995/3/3/pubmed PY - 1995/3/3/medline PY - 1995/3/3/entrez SP - 472 EP - 85 JF - Journal of molecular biology JO - J. Mol. Biol. VL - 246 IS - 4 N2 - A new model for copy number control of the plasmid R1 has been developed. It takes into account that initiation of replication of R1 requires a large number of cis-acting proteins (RepA). The theory explains how plasmid production rates respond to shifts in external conditions. It predicts the observed "eclipse" times between two plasmid duplications as well as the replication time for "runaway" plasmids lacking the antisense inhibitor CopA. The model also describes how the use of many cis-acting RepAs can lead to a tight coupling between cell and plasmid cycles that minimizes the rate of the plasmid loss. The results may be used as a guideline for construction of low copy number plasmids with high maintenance stability. SN - 0022-2836 UR - https://www.unboundmedicine.com/medline/citation/7877168/A_model_for_copy_number_control_of_the_plasmid_R1_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2836(84)70099-4 DB - PRIME DP - Unbound Medicine ER -