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Biphenotypic sarcomas with myogenic and neural differentiation express the Ewing's sarcoma EWS/FLI1 fusion gene.
Cancer Res. 1995 Mar 15; 55(6):1385-92.CR

Abstract

Accurate diagnosis of primitive childhood sarcomas continues to be a formidable problem because these malignancies generally demonstrate very little morphological evidence of their tissue of origin. One of these tumor classes, the Ewing's sarcoma family of peripheral primitive neuroectodermal tumors (pPNETs), are thought to have a neural histogenesis based on evidence of neuroectodermal differentiation. Greater than 95% of pPNETs carry t(11;22) or t(21;22) chromosomal translocations which fuse the EWS gene from chromosome 22q12 in-frame with either FLI1 from chromosome 11q24 or ERG from chromosome 21q22. The pPNETs are considered to be histogenetically distinct from rhabdomyosarcomas, myogenic tumors lacking these EWS gene fusions and hypothesized to derive from immature skeletal muscle precursors. In the present study, we describe a unique set of childhood soft tissue sarcomas that show both neural and myogenic differentiation. These biphenotypic tumors express myogenic regulatory factors and muscle-specific antigens and also show neuroectodermal differentiation with ultrastructural evidence of neurosecretory granules and expression of neural-associated genes. Northern analysis and reverse transcriptase PCR reveal expression of EWS/FLI1 gene fusions in all biphenotypic sarcomas analyzed. Chimeric EWS/FLI1 transcripts and fusion proteins in these tumors are identical to those described for pPNETs. Our results provide evidence for a class of biphenotypic childhood sarcomas with myogenic and neural differentiation and suggest that these tumors may be related to the Ewing's sarcoma family of pPNETs.

Authors+Show Affiliations

Department of Pathology and Laboratory Medicine, British Columbia's Children's Hospital/University of British Columbia, Vancouver, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

7882340

Citation

Sorensen, P H., et al. "Biphenotypic Sarcomas With Myogenic and Neural Differentiation Express the Ewing's Sarcoma EWS/FLI1 Fusion Gene." Cancer Research, vol. 55, no. 6, 1995, pp. 1385-92.
Sorensen PH, Shimada H, Liu XF, et al. Biphenotypic sarcomas with myogenic and neural differentiation express the Ewing's sarcoma EWS/FLI1 fusion gene. Cancer Res. 1995;55(6):1385-92.
Sorensen, P. H., Shimada, H., Liu, X. F., Lim, J. F., Thomas, G., & Triche, T. J. (1995). Biphenotypic sarcomas with myogenic and neural differentiation express the Ewing's sarcoma EWS/FLI1 fusion gene. Cancer Research, 55(6), 1385-92.
Sorensen PH, et al. Biphenotypic Sarcomas With Myogenic and Neural Differentiation Express the Ewing's Sarcoma EWS/FLI1 Fusion Gene. Cancer Res. 1995 Mar 15;55(6):1385-92. PubMed PMID: 7882340.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biphenotypic sarcomas with myogenic and neural differentiation express the Ewing's sarcoma EWS/FLI1 fusion gene. AU - Sorensen,P H, AU - Shimada,H, AU - Liu,X F, AU - Lim,J F, AU - Thomas,G, AU - Triche,T J, PY - 1995/3/15/pubmed PY - 1995/3/15/medline PY - 1995/3/15/entrez SP - 1385 EP - 92 JF - Cancer research JO - Cancer Res VL - 55 IS - 6 N2 - Accurate diagnosis of primitive childhood sarcomas continues to be a formidable problem because these malignancies generally demonstrate very little morphological evidence of their tissue of origin. One of these tumor classes, the Ewing's sarcoma family of peripheral primitive neuroectodermal tumors (pPNETs), are thought to have a neural histogenesis based on evidence of neuroectodermal differentiation. Greater than 95% of pPNETs carry t(11;22) or t(21;22) chromosomal translocations which fuse the EWS gene from chromosome 22q12 in-frame with either FLI1 from chromosome 11q24 or ERG from chromosome 21q22. The pPNETs are considered to be histogenetically distinct from rhabdomyosarcomas, myogenic tumors lacking these EWS gene fusions and hypothesized to derive from immature skeletal muscle precursors. In the present study, we describe a unique set of childhood soft tissue sarcomas that show both neural and myogenic differentiation. These biphenotypic tumors express myogenic regulatory factors and muscle-specific antigens and also show neuroectodermal differentiation with ultrastructural evidence of neurosecretory granules and expression of neural-associated genes. Northern analysis and reverse transcriptase PCR reveal expression of EWS/FLI1 gene fusions in all biphenotypic sarcomas analyzed. Chimeric EWS/FLI1 transcripts and fusion proteins in these tumors are identical to those described for pPNETs. Our results provide evidence for a class of biphenotypic childhood sarcomas with myogenic and neural differentiation and suggest that these tumors may be related to the Ewing's sarcoma family of pPNETs. SN - 0008-5472 UR - https://www.unboundmedicine.com/medline/citation/7882340/Biphenotypic_sarcomas_with_myogenic_and_neural_differentiation_express_the_Ewing's_sarcoma_EWS/FLI1_fusion_gene_ L2 - http://cancerres.aacrjournals.org/cgi/pmidlookup?view=long&pmid=7882340 DB - PRIME DP - Unbound Medicine ER -