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Genomic remnants of alpha-globin genes in the hemoglobinless antarctic icefishes.
Proc Natl Acad Sci U S A. 1995 Mar 14; 92(6):1817-21.PN

Abstract

Alone among piscine taxa, the antarctic icefishes (family Channichthyidae, suborder Notothenioidei) have evolved compensatory adaptations that maintain normal metabolic functions in the absence of erythrocytes and the respiratory oxygen transporter hemoglobin. Although the uniquely "colorless" or "white" condition of the blood of icefishes has been recognized since the early 20th century, the status of globin genes in the icefish genomes has, surprisingly, remained unexplored. Using alpha- and beta-globin cDNAs from the antarctic rockcod Notothenia coriiceps (family Nototheniidae, suborder Notothenioidei), we have probed the genomes of three white-blooded icefishes and four red-blooded notothenioid relatives (three antarctic, one temperate) for globin-related DNA sequences. We detect specific, high-stringency hybridization of the alpha-globin probe to genomic DNAs of both white- and red-blooded species, whereas the beta-globin cDNA hybridizes only to the genomes of the red-blooded fishes. Our results suggest that icefishes retain inactive genomic remnants of alpha-globin genes but have lost, either through deletion or through rapid mutation, the gene that encodes beta-globin. We propose that the hemoglobinless phenotype of extant icefishes is the result of deletion of the single adult beta-globin locus prior to the diversification of the clade.

Authors+Show Affiliations

Istituto di Biochimica delle Proteine ed Enzimologia, Consiglio Nazionale delle Ricerche, Naples, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

7892183

Citation

Cocca, E, et al. "Genomic Remnants of Alpha-globin Genes in the Hemoglobinless Antarctic Icefishes." Proceedings of the National Academy of Sciences of the United States of America, vol. 92, no. 6, 1995, pp. 1817-21.
Cocca E, Ratnayake-Lecamwasam M, Parker SK, et al. Genomic remnants of alpha-globin genes in the hemoglobinless antarctic icefishes. Proc Natl Acad Sci U S A. 1995;92(6):1817-21.
Cocca, E., Ratnayake-Lecamwasam, M., Parker, S. K., Camardella, L., Ciaramella, M., di Prisco, G., & Detrich, H. W. (1995). Genomic remnants of alpha-globin genes in the hemoglobinless antarctic icefishes. Proceedings of the National Academy of Sciences of the United States of America, 92(6), 1817-21.
Cocca E, et al. Genomic Remnants of Alpha-globin Genes in the Hemoglobinless Antarctic Icefishes. Proc Natl Acad Sci U S A. 1995 Mar 14;92(6):1817-21. PubMed PMID: 7892183.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genomic remnants of alpha-globin genes in the hemoglobinless antarctic icefishes. AU - Cocca,E, AU - Ratnayake-Lecamwasam,M, AU - Parker,S K, AU - Camardella,L, AU - Ciaramella,M, AU - di Prisco,G, AU - Detrich,H W,3rd PY - 1995/3/14/pubmed PY - 2001/3/28/medline PY - 1995/3/14/entrez SP - 1817 EP - 21 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 92 IS - 6 N2 - Alone among piscine taxa, the antarctic icefishes (family Channichthyidae, suborder Notothenioidei) have evolved compensatory adaptations that maintain normal metabolic functions in the absence of erythrocytes and the respiratory oxygen transporter hemoglobin. Although the uniquely "colorless" or "white" condition of the blood of icefishes has been recognized since the early 20th century, the status of globin genes in the icefish genomes has, surprisingly, remained unexplored. Using alpha- and beta-globin cDNAs from the antarctic rockcod Notothenia coriiceps (family Nototheniidae, suborder Notothenioidei), we have probed the genomes of three white-blooded icefishes and four red-blooded notothenioid relatives (three antarctic, one temperate) for globin-related DNA sequences. We detect specific, high-stringency hybridization of the alpha-globin probe to genomic DNAs of both white- and red-blooded species, whereas the beta-globin cDNA hybridizes only to the genomes of the red-blooded fishes. Our results suggest that icefishes retain inactive genomic remnants of alpha-globin genes but have lost, either through deletion or through rapid mutation, the gene that encodes beta-globin. We propose that the hemoglobinless phenotype of extant icefishes is the result of deletion of the single adult beta-globin locus prior to the diversification of the clade. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/7892183/Genomic_remnants_of_alpha_globin_genes_in_the_hemoglobinless_antarctic_icefishes_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=7892183 DB - PRIME DP - Unbound Medicine ER -