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Prediction of the rate of glucose utilization from cellular levels of glucose 6-phosphate and fructose 1,6-diphosphate in Escherichia coli.
J Bacteriol. 1976 Oct; 128(1):165-9.JB

Abstract

A comprehensive equation, upsilon = VM/[1 + (A0.5/Fru-P2)n] [ 1 + (Glc-6-P/I0.5)], has been proposed to represent the quantitative interrelationships between the rate of glucose utilization and the levels of glucose-6-phosphate and fructose-1,6-diphosphate in the intact Escherichia coli cell. This comprehensive equation was derived from empirical equations that describe the relationship between the rate of glucose utilization and one of these hexose phosphates in metabolic situations where the other hexoses phosphate was not altered. In the experiments described in this report, treatment of nitrogen (NH4+)-starved cultures of E. coli W4597 (K) with various concentrations of sodium azide altered the levels of both hexose phosphates as well as the rate of glucose utilization. In each case the observed rate and the rate predicted by the comprehensive equation agreed closely, substantiating the validity of this comprehensive relationship as a quantitative indicator of metabolic events in the intact cell. The mechanism of metabolic regulation that is represented by this equation is discussed in light of the cellular levels of adenosine 5'-triphosphate and phosphoenolpyruvate observed in these experiments.

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Authors

Dietzler DN
No affiliation info available
Leckie MP
No affiliation info available
Crimmins DL
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Ungar JM
No affiliation info available
Sternheim WL
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MeSH

Adenosine TriphosphateEscherichia coliFructosephosphatesGlucoseGlucosephosphatesKineticsPhosphoenolpyruvate

Pub Type(s)

Journal Article

Language

eng

PubMed ID

789330

Citation

Dietzler, D N., et al. "Prediction of the Rate of Glucose Utilization From Cellular Levels of Glucose 6-phosphate and Fructose 1,6-diphosphate in Escherichia Coli." Journal of Bacteriology, vol. 128, no. 1, 1976, pp. 165-9.
Dietzler DN, Leckie MP, Crimmins DL, et al. Prediction of the rate of glucose utilization from cellular levels of glucose 6-phosphate and fructose 1,6-diphosphate in Escherichia coli. J Bacteriol. 1976;128(1):165-9.
Dietzler, D. N., Leckie, M. P., Crimmins, D. L., Ungar, J. M., & Sternheim, W. L. (1976). Prediction of the rate of glucose utilization from cellular levels of glucose 6-phosphate and fructose 1,6-diphosphate in Escherichia coli. Journal of Bacteriology, 128(1), 165-9.
Dietzler DN, et al. Prediction of the Rate of Glucose Utilization From Cellular Levels of Glucose 6-phosphate and Fructose 1,6-diphosphate in Escherichia Coli. J Bacteriol. 1976;128(1):165-9. PubMed PMID: 789330.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prediction of the rate of glucose utilization from cellular levels of glucose 6-phosphate and fructose 1,6-diphosphate in Escherichia coli. AU - Dietzler,D N, AU - Leckie,M P, AU - Crimmins,D L, AU - Ungar,J M, AU - Sternheim,W L, PY - 1976/10/1/pubmed PY - 1976/10/1/medline PY - 1976/10/1/entrez SP - 165 EP - 9 JF - Journal of bacteriology JO - J Bacteriol VL - 128 IS - 1 N2 - A comprehensive equation, upsilon = VM/[1 + (A0.5/Fru-P2)n] [ 1 + (Glc-6-P/I0.5)], has been proposed to represent the quantitative interrelationships between the rate of glucose utilization and the levels of glucose-6-phosphate and fructose-1,6-diphosphate in the intact Escherichia coli cell. This comprehensive equation was derived from empirical equations that describe the relationship between the rate of glucose utilization and one of these hexose phosphates in metabolic situations where the other hexoses phosphate was not altered. In the experiments described in this report, treatment of nitrogen (NH4+)-starved cultures of E. coli W4597 (K) with various concentrations of sodium azide altered the levels of both hexose phosphates as well as the rate of glucose utilization. In each case the observed rate and the rate predicted by the comprehensive equation agreed closely, substantiating the validity of this comprehensive relationship as a quantitative indicator of metabolic events in the intact cell. The mechanism of metabolic regulation that is represented by this equation is discussed in light of the cellular levels of adenosine 5'-triphosphate and phosphoenolpyruvate observed in these experiments. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/789330/Prediction_of_the_rate_of_glucose_utilization_from_cellular_levels_of_glucose_6_phosphate_and_fructose_16_diphosphate_in_Escherichia_coli_ L2 - https://journals.asm.org/doi/10.1128/jb.128.1.165-169.1976?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -