Tags

Type your tag names separated by a space and hit enter

Determination of plasma mexiletine levels with gas chromatography-mass spectrometry and selected-ion monitoring.
J Chromatogr B Biomed Appl. 1994 Dec 02; 662(1):118-22.JC

Abstract

Mexiletine, 1-(2,6-dimethylphenoxy)-2-aminopropane (Mexitil), is an orally effective agent useful in the treatment of serious ventricular arrhythmias. This paper describes a gas chromatographic-mass spectrophotometric assay with selected-ion monitoring for the measurement of plasma or serum mexiletine levels. The drug and internal standard (p-chlorophenylalanine methyl ester) were extracted from plasma into ethyl acetate-hexane-methanol (60:40:1, v/v). After separation and evaporation of the organic phase, the drug and internal standard were derivatized to their pentafluoropropyl derivatives prior to analysis. The reproducibility of the daily standard curve yielded mean inter- and intra-day coefficients of variability from 0.7 to 11.0%. The coefficients of variability for control plasma samples (0.5 and 1.0 micrograms/ml) ranged from 2.6 to 5.0% and the accuracy of the assay was 106 +/- 6 and 100 +/- 5% for the low and high level controls respectively. The limit of quantitation for the assay was 0.1 micrograms/ml. No interfering peaks were detected in any patient samples. This method can be used as a primary analytical method to measure mexiletine plasma levels or can serve as a convenient back-up method to HPLC procedures when contaminating peaks coelute with mexiletine.

Authors+Show Affiliations

Pharmakon R and D Laboratories, Pittsburgh, PA 15213.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

7894684

Citation

Minnigh, M B., et al. "Determination of Plasma Mexiletine Levels With Gas Chromatography-mass Spectrometry and Selected-ion Monitoring." Journal of Chromatography. B, Biomedical Applications, vol. 662, no. 1, 1994, pp. 118-22.
Minnigh MB, Alvin JD, Zemaitis MA. Determination of plasma mexiletine levels with gas chromatography-mass spectrometry and selected-ion monitoring. J Chromatogr B, Biomed Appl. 1994;662(1):118-22.
Minnigh, M. B., Alvin, J. D., & Zemaitis, M. A. (1994). Determination of plasma mexiletine levels with gas chromatography-mass spectrometry and selected-ion monitoring. Journal of Chromatography. B, Biomedical Applications, 662(1), 118-22.
Minnigh MB, Alvin JD, Zemaitis MA. Determination of Plasma Mexiletine Levels With Gas Chromatography-mass Spectrometry and Selected-ion Monitoring. J Chromatogr B, Biomed Appl. 1994 Dec 2;662(1):118-22. PubMed PMID: 7894684.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of plasma mexiletine levels with gas chromatography-mass spectrometry and selected-ion monitoring. AU - Minnigh,M B, AU - Alvin,J D, AU - Zemaitis,M A, PY - 1994/12/2/pubmed PY - 1994/12/2/medline PY - 1994/12/2/entrez SP - 118 EP - 22 JF - Journal of chromatography. B, Biomedical applications JO - J. Chromatogr. B, Biomed. Appl. VL - 662 IS - 1 N2 - Mexiletine, 1-(2,6-dimethylphenoxy)-2-aminopropane (Mexitil), is an orally effective agent useful in the treatment of serious ventricular arrhythmias. This paper describes a gas chromatographic-mass spectrophotometric assay with selected-ion monitoring for the measurement of plasma or serum mexiletine levels. The drug and internal standard (p-chlorophenylalanine methyl ester) were extracted from plasma into ethyl acetate-hexane-methanol (60:40:1, v/v). After separation and evaporation of the organic phase, the drug and internal standard were derivatized to their pentafluoropropyl derivatives prior to analysis. The reproducibility of the daily standard curve yielded mean inter- and intra-day coefficients of variability from 0.7 to 11.0%. The coefficients of variability for control plasma samples (0.5 and 1.0 micrograms/ml) ranged from 2.6 to 5.0% and the accuracy of the assay was 106 +/- 6 and 100 +/- 5% for the low and high level controls respectively. The limit of quantitation for the assay was 0.1 micrograms/ml. No interfering peaks were detected in any patient samples. This method can be used as a primary analytical method to measure mexiletine plasma levels or can serve as a convenient back-up method to HPLC procedures when contaminating peaks coelute with mexiletine. SN - 1572-6495 UR - https://www.unboundmedicine.com/medline/citation/7894684/Determination_of_plasma_mexiletine_levels_with_gas_chromatography_mass_spectrometry_and_selected_ion_monitoring_ DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.