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Precursor cells of mouse endocrine pancreas coexpress insulin, glucagon and the neuronal proteins tyrosine hydroxylase and neuropeptide Y, but not pancreatic polypeptide.
Development. 1993 Aug; 118(4):1031-9.D

Abstract

The early progenitor cells to the pancreatic islets in the mouse have been characterized so as to re-examine their possible lineage relationships to the four islet cell types found in mature islets. Insulin and glucagon were both first expressed at embryonic day 9.5, and many cells coexpressed these two markers, as shown by light and electron microscopic analysis using double-label immunohistochemistry. Incubation of embryonic pancreas with 1% glutaraldehyde, a fixative commonly used by electron microscopists, abolished this reactivity, thereby explaining reported difficulties in detecting these precursor cells. Using antisera specific for neuropeptide Y (NPY) a peptide with considerable homology to pancreatic polypeptide (PP), we show that NPY first appears with insulin and glucagon immunoreactivity at E9.5, and is co-expressed with glucagon in a majority of adult alpha cells. As we have previously reported, PP itself is first detectable immunocytochemically at postnatal day 1 with PP-specific antibodies. However, antibodies raised against bovine PP are shown by dot blotting to recognize NPY with comparable avidity, indicating that a recent report of islet progenitor cells containing PP at E9.5 (Herrera, P. L., Huarte, J., Sanvito, F., Meda, P., Orci, L. and Vassalli, J. D. (1991) Development 113, 1257-1265), actually represents cross-reactivity to NPY. The data support a model in which early precursor cells to the endocrine pancreas co-activate and co-express a set of islet cell hormone and neural genes, whose expression is both selectively increased and extinguished as development proceeds, concomitant with a restriction to the patterns of expression characteristic of mature islet cell types.

Authors+Show Affiliations

Department of Anatomy and Cell Biology, SUNY Health Science Center, Brooklyn 11203.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

7903631

Citation

Teitelman, G, et al. "Precursor Cells of Mouse Endocrine Pancreas Coexpress Insulin, Glucagon and the Neuronal Proteins Tyrosine Hydroxylase and Neuropeptide Y, but Not Pancreatic Polypeptide." Development (Cambridge, England), vol. 118, no. 4, 1993, pp. 1031-9.
Teitelman G, Alpert S, Polak JM, et al. Precursor cells of mouse endocrine pancreas coexpress insulin, glucagon and the neuronal proteins tyrosine hydroxylase and neuropeptide Y, but not pancreatic polypeptide. Development. 1993;118(4):1031-9.
Teitelman, G., Alpert, S., Polak, J. M., Martinez, A., & Hanahan, D. (1993). Precursor cells of mouse endocrine pancreas coexpress insulin, glucagon and the neuronal proteins tyrosine hydroxylase and neuropeptide Y, but not pancreatic polypeptide. Development (Cambridge, England), 118(4), 1031-9.
Teitelman G, et al. Precursor Cells of Mouse Endocrine Pancreas Coexpress Insulin, Glucagon and the Neuronal Proteins Tyrosine Hydroxylase and Neuropeptide Y, but Not Pancreatic Polypeptide. Development. 1993;118(4):1031-9. PubMed PMID: 7903631.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Precursor cells of mouse endocrine pancreas coexpress insulin, glucagon and the neuronal proteins tyrosine hydroxylase and neuropeptide Y, but not pancreatic polypeptide. AU - Teitelman,G, AU - Alpert,S, AU - Polak,J M, AU - Martinez,A, AU - Hanahan,D, PY - 1993/8/1/pubmed PY - 1993/8/1/medline PY - 1993/8/1/entrez SP - 1031 EP - 9 JF - Development (Cambridge, England) JO - Development VL - 118 IS - 4 N2 - The early progenitor cells to the pancreatic islets in the mouse have been characterized so as to re-examine their possible lineage relationships to the four islet cell types found in mature islets. Insulin and glucagon were both first expressed at embryonic day 9.5, and many cells coexpressed these two markers, as shown by light and electron microscopic analysis using double-label immunohistochemistry. Incubation of embryonic pancreas with 1% glutaraldehyde, a fixative commonly used by electron microscopists, abolished this reactivity, thereby explaining reported difficulties in detecting these precursor cells. Using antisera specific for neuropeptide Y (NPY) a peptide with considerable homology to pancreatic polypeptide (PP), we show that NPY first appears with insulin and glucagon immunoreactivity at E9.5, and is co-expressed with glucagon in a majority of adult alpha cells. As we have previously reported, PP itself is first detectable immunocytochemically at postnatal day 1 with PP-specific antibodies. However, antibodies raised against bovine PP are shown by dot blotting to recognize NPY with comparable avidity, indicating that a recent report of islet progenitor cells containing PP at E9.5 (Herrera, P. L., Huarte, J., Sanvito, F., Meda, P., Orci, L. and Vassalli, J. D. (1991) Development 113, 1257-1265), actually represents cross-reactivity to NPY. The data support a model in which early precursor cells to the endocrine pancreas co-activate and co-express a set of islet cell hormone and neural genes, whose expression is both selectively increased and extinguished as development proceeds, concomitant with a restriction to the patterns of expression characteristic of mature islet cell types. SN - 0950-1991 UR - https://www.unboundmedicine.com/medline/citation/7903631/Precursor_cells_of_mouse_endocrine_pancreas_coexpress_insulin_glucagon_and_the_neuronal_proteins_tyrosine_hydroxylase_and_neuropeptide_Y_but_not_pancreatic_polypeptide_ L2 - http://dev.biologists.org/cgi/pmidlookup?view=long&pmid=7903631 DB - PRIME DP - Unbound Medicine ER -